Events leading to the opening and closing of the exocytotic fusion pore have markedly different temperature dependencies

Kinetic analysis of single fusion events in patch-clamped mouse mast cells

Andres Oberhauser, J. R. Monck, J. M. Fernandez

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The earliest event in exocytosis is the formation of a fusion pore, an aqueous channel that connects the lumen of a secretory granule with the extracellular space. We can observe the formation of individual fusion pores and their subsequent dilation or closure by measuring the changes in the admittance of patch-clamped mast cells during GTPγS-stimulated exocytotic fusion. To investigate the molecular structure of the fusion pore, we have studied the temperature dependency of the rate constants for fusion pore formation and closure. An Arrhenius plot of the rate of fusion pore formation shows a simple linear relationship with an apparent activation energy of 23 kcal/mol. In contrast, the Arrhenius plot of the rate of closure of the fusion pore is discontinuous, with the break at ~13°C. Above the break point, the rate of closure has a weak temperature dependence (7 kcal/mol), whereas below 13°C the rate of closure is temperature independent. This type of temperature dependency is characteristic of events that depend on diffusion in a lipid phase that undergoes a fluid-solid phase transition. We propose that the formation of the fusion pore is regulated by the conformational change of a molecular structure with a high activation energy, whereas the closure of the fusion pore is regulated by lipids that become phase separated at 13°C.

Original languageEnglish (US)
Pages (from-to)800-809
Number of pages10
JournalBiophysical Journal
Volume61
Issue number3
StatePublished - 1992
Externally publishedYes

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Mast Cells
Temperature
Molecular Structure
Lipids
Phase Transition
Exocytosis
Secretory Vesicles
Extracellular Space
Dilatation

ASJC Scopus subject areas

  • Biophysics

Cite this

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title = "Events leading to the opening and closing of the exocytotic fusion pore have markedly different temperature dependencies: Kinetic analysis of single fusion events in patch-clamped mouse mast cells",
abstract = "The earliest event in exocytosis is the formation of a fusion pore, an aqueous channel that connects the lumen of a secretory granule with the extracellular space. We can observe the formation of individual fusion pores and their subsequent dilation or closure by measuring the changes in the admittance of patch-clamped mast cells during GTPγS-stimulated exocytotic fusion. To investigate the molecular structure of the fusion pore, we have studied the temperature dependency of the rate constants for fusion pore formation and closure. An Arrhenius plot of the rate of fusion pore formation shows a simple linear relationship with an apparent activation energy of 23 kcal/mol. In contrast, the Arrhenius plot of the rate of closure of the fusion pore is discontinuous, with the break at ~13°C. Above the break point, the rate of closure has a weak temperature dependence (7 kcal/mol), whereas below 13°C the rate of closure is temperature independent. This type of temperature dependency is characteristic of events that depend on diffusion in a lipid phase that undergoes a fluid-solid phase transition. We propose that the formation of the fusion pore is regulated by the conformational change of a molecular structure with a high activation energy, whereas the closure of the fusion pore is regulated by lipids that become phase separated at 13°C.",
author = "Andres Oberhauser and Monck, {J. R.} and Fernandez, {J. M.}",
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T1 - Events leading to the opening and closing of the exocytotic fusion pore have markedly different temperature dependencies

T2 - Kinetic analysis of single fusion events in patch-clamped mouse mast cells

AU - Oberhauser, Andres

AU - Monck, J. R.

AU - Fernandez, J. M.

PY - 1992

Y1 - 1992

N2 - The earliest event in exocytosis is the formation of a fusion pore, an aqueous channel that connects the lumen of a secretory granule with the extracellular space. We can observe the formation of individual fusion pores and their subsequent dilation or closure by measuring the changes in the admittance of patch-clamped mast cells during GTPγS-stimulated exocytotic fusion. To investigate the molecular structure of the fusion pore, we have studied the temperature dependency of the rate constants for fusion pore formation and closure. An Arrhenius plot of the rate of fusion pore formation shows a simple linear relationship with an apparent activation energy of 23 kcal/mol. In contrast, the Arrhenius plot of the rate of closure of the fusion pore is discontinuous, with the break at ~13°C. Above the break point, the rate of closure has a weak temperature dependence (7 kcal/mol), whereas below 13°C the rate of closure is temperature independent. This type of temperature dependency is characteristic of events that depend on diffusion in a lipid phase that undergoes a fluid-solid phase transition. We propose that the formation of the fusion pore is regulated by the conformational change of a molecular structure with a high activation energy, whereas the closure of the fusion pore is regulated by lipids that become phase separated at 13°C.

AB - The earliest event in exocytosis is the formation of a fusion pore, an aqueous channel that connects the lumen of a secretory granule with the extracellular space. We can observe the formation of individual fusion pores and their subsequent dilation or closure by measuring the changes in the admittance of patch-clamped mast cells during GTPγS-stimulated exocytotic fusion. To investigate the molecular structure of the fusion pore, we have studied the temperature dependency of the rate constants for fusion pore formation and closure. An Arrhenius plot of the rate of fusion pore formation shows a simple linear relationship with an apparent activation energy of 23 kcal/mol. In contrast, the Arrhenius plot of the rate of closure of the fusion pore is discontinuous, with the break at ~13°C. Above the break point, the rate of closure has a weak temperature dependence (7 kcal/mol), whereas below 13°C the rate of closure is temperature independent. This type of temperature dependency is characteristic of events that depend on diffusion in a lipid phase that undergoes a fluid-solid phase transition. We propose that the formation of the fusion pore is regulated by the conformational change of a molecular structure with a high activation energy, whereas the closure of the fusion pore is regulated by lipids that become phase separated at 13°C.

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