Evidence for decreased calcitonin gene-related peptide (CGRP) receptors and compromised responsiveness to CGRP of fetoplacental vessels in preeclamptic pregnancies

Yuan Lin Dong, Kortney E. Green, Sujatha Vegiragu, Gary Hankins, Dora Martin, Madhu Chauhan, Chandrasekhar Thota, Chandrasekhar Yallampalli

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) is a potent vasodilatory peptide, and its concentration is increased in both maternal and fetal circulation during late pregnancy. The present study was designed to investigate the expression of CGRP receptor components, calcitonin receptor-like receptor (CRLR), and receptor activity modifying protein 1 (RAMP1), and the relaxation response to CGRP in fetoplacental vessels from normotensive pregnant women and women with preeclampsia. Results showed that: 1) mRNA for both CRLR and RAMP1 was expressed in fetoplacental vessels from normal pregnancies; however, these mRNA expressions were substantially reduced in the vessels from preeclamptic women; 2) CRLR and RAMP1 proteins were abundantly expressed in the endothelium and smooth muscle layer of the fetoplacental vessels, as well as the trophoblast cells in normal placentas. In contrast, both vascular tissues and trophoblasts showed decreased expressions for CRLR and RAMP1 proteins and declined CGRP binding sites in preeclamptic placentas; and 3) CGRP produced a dose-dependent relaxation of serotonin-induced contraction of umbilical and chorionic arteries from normal pregnancies, but the response to CGRP was significantly attenuated in the vessels from preeclampsia. We concluded that CGRP may contribute to the low fetoplacental vascular resistance in normal pregnancies; however, CGRP-dependent vascular relaxation appears to be compromised in preeclamptic pregnancies.

Original languageEnglish (US)
Pages (from-to)2336-2343
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number4
DOIs
StatePublished - Apr 2005

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Calcitonin Gene-Related Peptide Receptors
Calcitonin Gene-Related Peptide
Receptor Activity-Modifying Protein 1
Calcitonin Receptor-Like Protein
Pregnancy
Trophoblasts
Pre-Eclampsia
Placenta
Blood Vessels
Messenger RNA
Umbilical Arteries
Vascular Resistance
Endothelium
Smooth Muscle
Muscle
Pregnant Women
Serotonin
Proteins
Binding Sites
Mothers

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Evidence for decreased calcitonin gene-related peptide (CGRP) receptors and compromised responsiveness to CGRP of fetoplacental vessels in preeclamptic pregnancies. / Dong, Yuan Lin; Green, Kortney E.; Vegiragu, Sujatha; Hankins, Gary; Martin, Dora; Chauhan, Madhu; Thota, Chandrasekhar; Yallampalli, Chandrasekhar.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 4, 04.2005, p. 2336-2343.

Research output: Contribution to journalArticle

Dong, Yuan Lin ; Green, Kortney E. ; Vegiragu, Sujatha ; Hankins, Gary ; Martin, Dora ; Chauhan, Madhu ; Thota, Chandrasekhar ; Yallampalli, Chandrasekhar. / Evidence for decreased calcitonin gene-related peptide (CGRP) receptors and compromised responsiveness to CGRP of fetoplacental vessels in preeclamptic pregnancies. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 4. pp. 2336-2343.
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