Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants

T. H. Risby, W. Maley, R. P W Scott, G. B. Bulkley, M. Kazui, S. S. Schnert, K. B. Schwarz, J. Potter, E. Mezey, A. S. Klein, P. Colombani, Jeffrey Fair, W. T. Merritt, C. Beattie, M. C. Mitchell, G. M. Williams, B. A. Perler, R. T. Donham, J. F. Burdick

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Background. Generation of toxic oxygen metabolites at reperfusion may contribute to the injury sustained as a consequence of harvest and ischemic preservation of organ allografts. Because there is a paucity of evidence that this mechanism is operative in human beings, we measured the generation of ethane into the exhaled breath as a biomarker of free radical-mediated lipid peroxidation in human liver transplantation. Methods. A novel technique that increased the previous standard of sensitivity 100-fold was used to measure picomole quantities of ethane in exhaled breath of eight recipients undergoing human orthotopic liver transplantation. Results. Ethane production correlated closely with the specific events of liver transplantation including the initial reperfusion of the allografts. In every case a twofold to threefold increase in ethane production was superimposed on a stable baseline immediately after reestablishment of portal vein blood flow through the donor liver. Conclusions. Ethane production was interpreted as evidence of hepatic lipid peroxidation, presumably mediated by toxic metabolites of oxygen occurring at reperfusion. This noninvasive approach allowed localization of the time point at which lipid peroxidation occurred and may facilitate quantification of lipid peroxidation mediated by free radicals and other toxic oxygen metabolites during operation.

Original languageEnglish (US)
Pages (from-to)91-101
Number of pages11
JournalSurgery
Volume115
Issue number1
StatePublished - 1994
Externally publishedYes

Fingerprint

Ethane
Lipid Peroxidation
Reperfusion
Free Radicals
Poisons
Transplants
Liver Transplantation
Liver
Oxygen
Allografts
Organ Preservation
Portal Vein
Biomarkers
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Risby, T. H., Maley, W., Scott, R. P. W., Bulkley, G. B., Kazui, M., Schnert, S. S., ... Burdick, J. F. (1994). Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants. Surgery, 115(1), 91-101.

Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants. / Risby, T. H.; Maley, W.; Scott, R. P W; Bulkley, G. B.; Kazui, M.; Schnert, S. S.; Schwarz, K. B.; Potter, J.; Mezey, E.; Klein, A. S.; Colombani, P.; Fair, Jeffrey; Merritt, W. T.; Beattie, C.; Mitchell, M. C.; Williams, G. M.; Perler, B. A.; Donham, R. T.; Burdick, J. F.

In: Surgery, Vol. 115, No. 1, 1994, p. 91-101.

Research output: Contribution to journalArticle

Risby, TH, Maley, W, Scott, RPW, Bulkley, GB, Kazui, M, Schnert, SS, Schwarz, KB, Potter, J, Mezey, E, Klein, AS, Colombani, P, Fair, J, Merritt, WT, Beattie, C, Mitchell, MC, Williams, GM, Perler, BA, Donham, RT & Burdick, JF 1994, 'Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants', Surgery, vol. 115, no. 1, pp. 91-101.
Risby TH, Maley W, Scott RPW, Bulkley GB, Kazui M, Schnert SS et al. Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants. Surgery. 1994;115(1):91-101.
Risby, T. H. ; Maley, W. ; Scott, R. P W ; Bulkley, G. B. ; Kazui, M. ; Schnert, S. S. ; Schwarz, K. B. ; Potter, J. ; Mezey, E. ; Klein, A. S. ; Colombani, P. ; Fair, Jeffrey ; Merritt, W. T. ; Beattie, C. ; Mitchell, M. C. ; Williams, G. M. ; Perler, B. A. ; Donham, R. T. ; Burdick, J. F. / Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants. In: Surgery. 1994 ; Vol. 115, No. 1. pp. 91-101.
@article{355509a18f3e407bbf6dfab52f5683fc,
title = "Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants",
abstract = "Background. Generation of toxic oxygen metabolites at reperfusion may contribute to the injury sustained as a consequence of harvest and ischemic preservation of organ allografts. Because there is a paucity of evidence that this mechanism is operative in human beings, we measured the generation of ethane into the exhaled breath as a biomarker of free radical-mediated lipid peroxidation in human liver transplantation. Methods. A novel technique that increased the previous standard of sensitivity 100-fold was used to measure picomole quantities of ethane in exhaled breath of eight recipients undergoing human orthotopic liver transplantation. Results. Ethane production correlated closely with the specific events of liver transplantation including the initial reperfusion of the allografts. In every case a twofold to threefold increase in ethane production was superimposed on a stable baseline immediately after reestablishment of portal vein blood flow through the donor liver. Conclusions. Ethane production was interpreted as evidence of hepatic lipid peroxidation, presumably mediated by toxic metabolites of oxygen occurring at reperfusion. This noninvasive approach allowed localization of the time point at which lipid peroxidation occurred and may facilitate quantification of lipid peroxidation mediated by free radicals and other toxic oxygen metabolites during operation.",
author = "Risby, {T. H.} and W. Maley and Scott, {R. P W} and Bulkley, {G. B.} and M. Kazui and Schnert, {S. S.} and Schwarz, {K. B.} and J. Potter and E. Mezey and Klein, {A. S.} and P. Colombani and Jeffrey Fair and Merritt, {W. T.} and C. Beattie and Mitchell, {M. C.} and Williams, {G. M.} and Perler, {B. A.} and Donham, {R. T.} and Burdick, {J. F.}",
year = "1994",
language = "English (US)",
volume = "115",
pages = "91--101",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "1",

}

TY - JOUR

T1 - Evidence for free radical-mediated lipid peroxidation at reperfusion of human orthotopic liver transplants

AU - Risby, T. H.

AU - Maley, W.

AU - Scott, R. P W

AU - Bulkley, G. B.

AU - Kazui, M.

AU - Schnert, S. S.

AU - Schwarz, K. B.

AU - Potter, J.

AU - Mezey, E.

AU - Klein, A. S.

AU - Colombani, P.

AU - Fair, Jeffrey

AU - Merritt, W. T.

AU - Beattie, C.

AU - Mitchell, M. C.

AU - Williams, G. M.

AU - Perler, B. A.

AU - Donham, R. T.

AU - Burdick, J. F.

PY - 1994

Y1 - 1994

N2 - Background. Generation of toxic oxygen metabolites at reperfusion may contribute to the injury sustained as a consequence of harvest and ischemic preservation of organ allografts. Because there is a paucity of evidence that this mechanism is operative in human beings, we measured the generation of ethane into the exhaled breath as a biomarker of free radical-mediated lipid peroxidation in human liver transplantation. Methods. A novel technique that increased the previous standard of sensitivity 100-fold was used to measure picomole quantities of ethane in exhaled breath of eight recipients undergoing human orthotopic liver transplantation. Results. Ethane production correlated closely with the specific events of liver transplantation including the initial reperfusion of the allografts. In every case a twofold to threefold increase in ethane production was superimposed on a stable baseline immediately after reestablishment of portal vein blood flow through the donor liver. Conclusions. Ethane production was interpreted as evidence of hepatic lipid peroxidation, presumably mediated by toxic metabolites of oxygen occurring at reperfusion. This noninvasive approach allowed localization of the time point at which lipid peroxidation occurred and may facilitate quantification of lipid peroxidation mediated by free radicals and other toxic oxygen metabolites during operation.

AB - Background. Generation of toxic oxygen metabolites at reperfusion may contribute to the injury sustained as a consequence of harvest and ischemic preservation of organ allografts. Because there is a paucity of evidence that this mechanism is operative in human beings, we measured the generation of ethane into the exhaled breath as a biomarker of free radical-mediated lipid peroxidation in human liver transplantation. Methods. A novel technique that increased the previous standard of sensitivity 100-fold was used to measure picomole quantities of ethane in exhaled breath of eight recipients undergoing human orthotopic liver transplantation. Results. Ethane production correlated closely with the specific events of liver transplantation including the initial reperfusion of the allografts. In every case a twofold to threefold increase in ethane production was superimposed on a stable baseline immediately after reestablishment of portal vein blood flow through the donor liver. Conclusions. Ethane production was interpreted as evidence of hepatic lipid peroxidation, presumably mediated by toxic metabolites of oxygen occurring at reperfusion. This noninvasive approach allowed localization of the time point at which lipid peroxidation occurred and may facilitate quantification of lipid peroxidation mediated by free radicals and other toxic oxygen metabolites during operation.

UR - http://www.scopus.com/inward/record.url?scp=0028090710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028090710&partnerID=8YFLogxK

M3 - Article

C2 - 8284767

AN - SCOPUS:0028090710

VL - 115

SP - 91

EP - 101

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 1

ER -