Evidence for nonregulatory trehalase activity in Dictyostelium discoideum

Jyothi Gupta; Steven D. Harris; David A. Cotter

doi:10.1007/BF01588179

Evidence for nonregulatory trehalase activity in Dictyostelium discoideum

Jyothi Gupta, Steven D. Harris, David A. Cotter

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

An in vitro activation treatment, stimulatory to the regulatory cytoplasmic trehalase of Saccharomyces cerevisiae, had no effect on the lysosomal trehalase of Dictyostelium discoideum. Concentrations of cAMP that produced a 19 to 22-fold increase in trehalase activity in S. cerevisiae extracts did not stimulate trehalase activity in D. discoideum extracts.. cGMP and 5′-AMP were also not effective in activating the enzyme. Dictyostelium discoideum trehalase exhibits characteristics typical of nonregulatory trehalases, in agreement with its lysosomal localization. The data are consistent with the hypothesis that changes in compartmentation regulate trehalose mobilization in D. discoideum.

Original language	English (US)
Pages (from-to)	101-104
Number of pages	4
Journal	Current Microbiology
Volume	16
Issue number	2
DOIs	https://doi.org/10.1007/BF01588179
State	Published - Mar 1987
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

Access to Document

10.1007/BF01588179

Cite this

@article{b79a54dc9bdb4cebbc630d7b52339280,

title = "Evidence for nonregulatory trehalase activity in Dictyostelium discoideum",

abstract = "An in vitro activation treatment, stimulatory to the regulatory cytoplasmic trehalase of Saccharomyces cerevisiae, had no effect on the lysosomal trehalase of Dictyostelium discoideum. Concentrations of cAMP that produced a 19 to 22-fold increase in trehalase activity in S. cerevisiae extracts did not stimulate trehalase activity in D. discoideum extracts.. cGMP and 5′-AMP were also not effective in activating the enzyme. Dictyostelium discoideum trehalase exhibits characteristics typical of nonregulatory trehalases, in agreement with its lysosomal localization. The data are consistent with the hypothesis that changes in compartmentation regulate trehalose mobilization in D. discoideum.",

author = "Jyothi Gupta and Harris, {Steven D.} and Cotter, {David A.}",

year = "1987",

month = mar,

doi = "10.1007/BF01588179",

language = "English (US)",

volume = "16",

pages = "101--104",

journal = "Current Microbiology",

issn = "0343-8651",

publisher = "Springer New York",

number = "2",

}

TY - JOUR

T1 - Evidence for nonregulatory trehalase activity in Dictyostelium discoideum

AU - Gupta, Jyothi

AU - Harris, Steven D.

AU - Cotter, David A.

PY - 1987/3

Y1 - 1987/3

N2 - An in vitro activation treatment, stimulatory to the regulatory cytoplasmic trehalase of Saccharomyces cerevisiae, had no effect on the lysosomal trehalase of Dictyostelium discoideum. Concentrations of cAMP that produced a 19 to 22-fold increase in trehalase activity in S. cerevisiae extracts did not stimulate trehalase activity in D. discoideum extracts.. cGMP and 5′-AMP were also not effective in activating the enzyme. Dictyostelium discoideum trehalase exhibits characteristics typical of nonregulatory trehalases, in agreement with its lysosomal localization. The data are consistent with the hypothesis that changes in compartmentation regulate trehalose mobilization in D. discoideum.

AB - An in vitro activation treatment, stimulatory to the regulatory cytoplasmic trehalase of Saccharomyces cerevisiae, had no effect on the lysosomal trehalase of Dictyostelium discoideum. Concentrations of cAMP that produced a 19 to 22-fold increase in trehalase activity in S. cerevisiae extracts did not stimulate trehalase activity in D. discoideum extracts.. cGMP and 5′-AMP were also not effective in activating the enzyme. Dictyostelium discoideum trehalase exhibits characteristics typical of nonregulatory trehalases, in agreement with its lysosomal localization. The data are consistent with the hypothesis that changes in compartmentation regulate trehalose mobilization in D. discoideum.

UR - http://www.scopus.com/inward/record.url?scp=0009496378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0009496378&partnerID=8YFLogxK

U2 - 10.1007/BF01588179

DO - 10.1007/BF01588179

M3 - Article

AN - SCOPUS:0009496378

SN - 0343-8651

VL - 16

SP - 101

EP - 104

JO - Current Microbiology

JF - Current Microbiology

IS - 2

ER -

Evidence for nonregulatory trehalase activity in Dictyostelium discoideum

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this