Evidence of a role for permeability factors in the pathogenesis of salmonellosis.

Johnny Peterson, P. D. Sandefur

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Two clinical isolates of Salmonella typhimurium were shown to produce two skin permeability factors. One factor was heat stable and rapid in onset while the other was heat labile and elicited maximal induration by 18 to 24 hr. The rapid, erythematous permeability factor (PF) response could not be prevented by antisera to cholera toxin or Salmonella antisomatic serum, but it could be simulated by high concentrations of lipopolysaccharide from S. typhimurium. The appearance of the delayed PF reaction was indistinguishable from that of purified cholera toxin. Histological comparisons of rabbit skin injected with Salmonella-delayed PF and cholera toxin revealed that both toxins resulted in gross edema and infiltration of polymorphonuclear leukocytes after 18 hr. The Salmonella-delayed PF was shown to be resistant to a variety of enzymes, sensitive to extremes in pH, and had an isoelectric point of pH 4.8. Unlike Salmonella lipopolysaccharide skin activity, the Salmonella-delayed PF was destroyed at 100 C and was neutralized by monospecific cholera antitoxin. The Salmonella-delayed PF, which shares antigenic determinants with cholera toxin, appears to be elaborated by living S. typhimurium cells in the rabbit ligated intestine, since rabbits immunized with procholeragenoid were protected against fluid loss from live cell challenge. Finally, production of the rapid PF is a stable genetic trait, while delayed PF production is apparently an unstable characteristic among the salmonellae.

Original languageEnglish (US)
Pages (from-to)197-209
Number of pages13
JournalAmerican Journal of Clinical Nutrition
Volume32
Issue number1
StatePublished - Jan 1979
Externally publishedYes

Fingerprint

Salmonella Infections
salmonellosis
Permeability
permeability
pathogenesis
Salmonella
Cholera Toxin
cholera toxin
Salmonella typhimurium
Salmonella Typhimurium
skin (animal)
rabbits
Rabbits
Skin
lipopolysaccharides
Lipopolysaccharides
Hot Temperature
antitoxins
Antitoxins
heat

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Evidence of a role for permeability factors in the pathogenesis of salmonellosis. / Peterson, Johnny; Sandefur, P. D.

In: American Journal of Clinical Nutrition, Vol. 32, No. 1, 01.1979, p. 197-209.

Research output: Contribution to journalArticle

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