Evidence of increased flux to n-6 docosapentaenoic acid in phospholipids of pancreas from cftr-/- knockout mice

Mario Ollero, Michael Laposata, Munir M. Zaman, Paola G. Blanco, Charlotte Andersson, John Zeind, Yana Urman, Geraldine Kent, Juan G. Alvarez, Steven D. Freedman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

An association has been reported between alterations in fatty acid metabolism and cystic fibrosis (CF). We hypothesized that these alterations are specific for a particular lipid component(s) and are the result of a specific metabolic defect. The different lipid classes were examined for fatty acid changes by using pancreatic homogenates and primary cultures of pancreatic acini from cftr-/- (CF) and wild-type mice. Lipid classes and phospholipids were separated by aminopropyl column chromatography and high-performance liquid chromatography, and fatty acid methyl esters were analyzed. The results indicate that in CF mice (1) linoleate was decreased in phospholipids but not in neutral lipids; (2) there was an increase in dihomo-γ-linolenate and in docosapentaenoate, the terminal fatty acid of the n-6 pathway, in total lipids and total phospholipids, but not in the neutral lipid class; and (3) the docosapentaenoate (n-6)/docosahexaenoate (n-3) ratio was significantly elevated in neutral phospholipids. This suggests an enhanced flux through the n-6 pathway beyond arachidonate. This study provides a more in-depth understanding of the fatty acid alterations found in CF, as reflected by the cftr-/- mouse model.

Original languageEnglish (US)
Pages (from-to)1192-1200
Number of pages9
JournalMetabolism: Clinical and Experimental
Volume55
Issue number9
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Knockout Mice
Pancreas
Phospholipids
Lipids
Fatty Acids
Cystic Fibrosis
alpha-Linolenic Acid
Docosahexaenoic Acids
Linoleic Acid
docosapentaenoic acid
Chromatography
Esters
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Evidence of increased flux to n-6 docosapentaenoic acid in phospholipids of pancreas from cftr-/- knockout mice. / Ollero, Mario; Laposata, Michael; Zaman, Munir M.; Blanco, Paola G.; Andersson, Charlotte; Zeind, John; Urman, Yana; Kent, Geraldine; Alvarez, Juan G.; Freedman, Steven D.

In: Metabolism: Clinical and Experimental, Vol. 55, No. 9, 09.2006, p. 1192-1200.

Research output: Contribution to journalArticle

Ollero, M, Laposata, M, Zaman, MM, Blanco, PG, Andersson, C, Zeind, J, Urman, Y, Kent, G, Alvarez, JG & Freedman, SD 2006, 'Evidence of increased flux to n-6 docosapentaenoic acid in phospholipids of pancreas from cftr-/- knockout mice', Metabolism: Clinical and Experimental, vol. 55, no. 9, pp. 1192-1200. https://doi.org/10.1016/j.metabol.2006.05.002
Ollero, Mario ; Laposata, Michael ; Zaman, Munir M. ; Blanco, Paola G. ; Andersson, Charlotte ; Zeind, John ; Urman, Yana ; Kent, Geraldine ; Alvarez, Juan G. ; Freedman, Steven D. / Evidence of increased flux to n-6 docosapentaenoic acid in phospholipids of pancreas from cftr-/- knockout mice. In: Metabolism: Clinical and Experimental. 2006 ; Vol. 55, No. 9. pp. 1192-1200.
@article{6f3af6cb4aaa404ca835c22a845b8b61,
title = "Evidence of increased flux to n-6 docosapentaenoic acid in phospholipids of pancreas from cftr-/- knockout mice",
abstract = "An association has been reported between alterations in fatty acid metabolism and cystic fibrosis (CF). We hypothesized that these alterations are specific for a particular lipid component(s) and are the result of a specific metabolic defect. The different lipid classes were examined for fatty acid changes by using pancreatic homogenates and primary cultures of pancreatic acini from cftr-/- (CF) and wild-type mice. Lipid classes and phospholipids were separated by aminopropyl column chromatography and high-performance liquid chromatography, and fatty acid methyl esters were analyzed. The results indicate that in CF mice (1) linoleate was decreased in phospholipids but not in neutral lipids; (2) there was an increase in dihomo-γ-linolenate and in docosapentaenoate, the terminal fatty acid of the n-6 pathway, in total lipids and total phospholipids, but not in the neutral lipid class; and (3) the docosapentaenoate (n-6)/docosahexaenoate (n-3) ratio was significantly elevated in neutral phospholipids. This suggests an enhanced flux through the n-6 pathway beyond arachidonate. This study provides a more in-depth understanding of the fatty acid alterations found in CF, as reflected by the cftr-/- mouse model.",
author = "Mario Ollero and Michael Laposata and Zaman, {Munir M.} and Blanco, {Paola G.} and Charlotte Andersson and John Zeind and Yana Urman and Geraldine Kent and Alvarez, {Juan G.} and Freedman, {Steven D.}",
year = "2006",
month = "9",
doi = "10.1016/j.metabol.2006.05.002",
language = "English (US)",
volume = "55",
pages = "1192--1200",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "9",

}

TY - JOUR

T1 - Evidence of increased flux to n-6 docosapentaenoic acid in phospholipids of pancreas from cftr-/- knockout mice

AU - Ollero, Mario

AU - Laposata, Michael

AU - Zaman, Munir M.

AU - Blanco, Paola G.

AU - Andersson, Charlotte

AU - Zeind, John

AU - Urman, Yana

AU - Kent, Geraldine

AU - Alvarez, Juan G.

AU - Freedman, Steven D.

PY - 2006/9

Y1 - 2006/9

N2 - An association has been reported between alterations in fatty acid metabolism and cystic fibrosis (CF). We hypothesized that these alterations are specific for a particular lipid component(s) and are the result of a specific metabolic defect. The different lipid classes were examined for fatty acid changes by using pancreatic homogenates and primary cultures of pancreatic acini from cftr-/- (CF) and wild-type mice. Lipid classes and phospholipids were separated by aminopropyl column chromatography and high-performance liquid chromatography, and fatty acid methyl esters were analyzed. The results indicate that in CF mice (1) linoleate was decreased in phospholipids but not in neutral lipids; (2) there was an increase in dihomo-γ-linolenate and in docosapentaenoate, the terminal fatty acid of the n-6 pathway, in total lipids and total phospholipids, but not in the neutral lipid class; and (3) the docosapentaenoate (n-6)/docosahexaenoate (n-3) ratio was significantly elevated in neutral phospholipids. This suggests an enhanced flux through the n-6 pathway beyond arachidonate. This study provides a more in-depth understanding of the fatty acid alterations found in CF, as reflected by the cftr-/- mouse model.

AB - An association has been reported between alterations in fatty acid metabolism and cystic fibrosis (CF). We hypothesized that these alterations are specific for a particular lipid component(s) and are the result of a specific metabolic defect. The different lipid classes were examined for fatty acid changes by using pancreatic homogenates and primary cultures of pancreatic acini from cftr-/- (CF) and wild-type mice. Lipid classes and phospholipids were separated by aminopropyl column chromatography and high-performance liquid chromatography, and fatty acid methyl esters were analyzed. The results indicate that in CF mice (1) linoleate was decreased in phospholipids but not in neutral lipids; (2) there was an increase in dihomo-γ-linolenate and in docosapentaenoate, the terminal fatty acid of the n-6 pathway, in total lipids and total phospholipids, but not in the neutral lipid class; and (3) the docosapentaenoate (n-6)/docosahexaenoate (n-3) ratio was significantly elevated in neutral phospholipids. This suggests an enhanced flux through the n-6 pathway beyond arachidonate. This study provides a more in-depth understanding of the fatty acid alterations found in CF, as reflected by the cftr-/- mouse model.

UR - http://www.scopus.com/inward/record.url?scp=33747074048&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747074048&partnerID=8YFLogxK

U2 - 10.1016/j.metabol.2006.05.002

DO - 10.1016/j.metabol.2006.05.002

M3 - Article

VL - 55

SP - 1192

EP - 1200

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 9

ER -