Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM

Gabriele Perriello, Simone Pampanelli, Paola Del Sindaco, Carlo Lalli, Marco Ciofetta, Elena Volpi, Fausto Santeusanio, Paolo Brunetti, Geremia B. Bolli

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

To assess the mechanisms of fasting hyperglycemia in NIDDM patients with mild elevation of fasting plasma glucose (FPG) compared with NIDDM patients with overt hyperglycemia, we studied 29 patients with NIDDM, who were divided in two groups according to their fasting plasma glucose (<7.8 and ≤7.8 mmol/l for groups A and B, respectively), and 16 control subjects who were matched with NIDDM patients for age, sex, and body mass index. All subjects were infused with [3-3H]glucose between 10:00 P.M. and 10:00 A.M. during overnight fasting to determine glucose fluxes. In 27 subjects (17 diabetic and 10 control), [U-14C]alanine was simultaneously infused between 4:00 A.M. and 10:00 A.M. to measure gluconeogenesis (GNG) from alanine. Arterialized-venous plasma samples were collected every 30 min for measurement of glucose fluxes, GNG, and glucoregulatory hormones. In group A, plasma glucose, rate of systemic glucose production (SGP), and GNG were greater than in control subjects (7.2 ± 0.2 vs. 4.9 ± 0.1 mmol/l, 109 ± 02 vs. 9.5 ± 03 μmol · kg-1 · min-1 and 0.58 ± 0.04 vs. 0.37 ± 0.02 μmol · kg-1 · min-1, respectively, for group A and control subjects; mean value 8:00 A.M.-10:00 A.M., all P < 0.05). Both increased SGP and GNG correlated with plasma glucose in all subjects (r = 0.77 and r = 0.75, respectively, P < 0.005). Plasma counterregulatory hormones did not differ in NIDDM patients compared to control subjects. The present studies demonstrate that SGP and GNG are increased in NIDDM patients without overt fasting hyperglycemia. Thus these metabolic abnormalities primarily contribute to early development of overnight and fasting hyperglycemia in NIDDM.

Original languageEnglish (US)
Pages (from-to)1010-1016
Number of pages7
JournalDiabetes
Volume46
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

Gluconeogenesis
Type 2 Diabetes Mellitus
Glucose
Fasting
Hyperglycemia
Alanine
Hormones
Body Mass Index
Control Groups

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Perriello, G., Pampanelli, S., Del Sindaco, P., Lalli, C., Ciofetta, M., Volpi, E., ... Bolli, G. B. (1997). Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM. Diabetes, 46(6), 1010-1016.

Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM. / Perriello, Gabriele; Pampanelli, Simone; Del Sindaco, Paola; Lalli, Carlo; Ciofetta, Marco; Volpi, Elena; Santeusanio, Fausto; Brunetti, Paolo; Bolli, Geremia B.

In: Diabetes, Vol. 46, No. 6, 1997, p. 1010-1016.

Research output: Contribution to journalArticle

Perriello, G, Pampanelli, S, Del Sindaco, P, Lalli, C, Ciofetta, M, Volpi, E, Santeusanio, F, Brunetti, P & Bolli, GB 1997, 'Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM', Diabetes, vol. 46, no. 6, pp. 1010-1016.
Perriello G, Pampanelli S, Del Sindaco P, Lalli C, Ciofetta M, Volpi E et al. Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM. Diabetes. 1997;46(6):1010-1016.
Perriello, Gabriele ; Pampanelli, Simone ; Del Sindaco, Paola ; Lalli, Carlo ; Ciofetta, Marco ; Volpi, Elena ; Santeusanio, Fausto ; Brunetti, Paolo ; Bolli, Geremia B. / Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM. In: Diabetes. 1997 ; Vol. 46, No. 6. pp. 1010-1016.
@article{4b7776e51a174713ad4c6bfc6dba53ed,
title = "Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM",
abstract = "To assess the mechanisms of fasting hyperglycemia in NIDDM patients with mild elevation of fasting plasma glucose (FPG) compared with NIDDM patients with overt hyperglycemia, we studied 29 patients with NIDDM, who were divided in two groups according to their fasting plasma glucose (<7.8 and ≤7.8 mmol/l for groups A and B, respectively), and 16 control subjects who were matched with NIDDM patients for age, sex, and body mass index. All subjects were infused with [3-3H]glucose between 10:00 P.M. and 10:00 A.M. during overnight fasting to determine glucose fluxes. In 27 subjects (17 diabetic and 10 control), [U-14C]alanine was simultaneously infused between 4:00 A.M. and 10:00 A.M. to measure gluconeogenesis (GNG) from alanine. Arterialized-venous plasma samples were collected every 30 min for measurement of glucose fluxes, GNG, and glucoregulatory hormones. In group A, plasma glucose, rate of systemic glucose production (SGP), and GNG were greater than in control subjects (7.2 ± 0.2 vs. 4.9 ± 0.1 mmol/l, 109 ± 02 vs. 9.5 ± 03 μmol · kg-1 · min-1 and 0.58 ± 0.04 vs. 0.37 ± 0.02 μmol · kg-1 · min-1, respectively, for group A and control subjects; mean value 8:00 A.M.-10:00 A.M., all P < 0.05). Both increased SGP and GNG correlated with plasma glucose in all subjects (r = 0.77 and r = 0.75, respectively, P < 0.005). Plasma counterregulatory hormones did not differ in NIDDM patients compared to control subjects. The present studies demonstrate that SGP and GNG are increased in NIDDM patients without overt fasting hyperglycemia. Thus these metabolic abnormalities primarily contribute to early development of overnight and fasting hyperglycemia in NIDDM.",
author = "Gabriele Perriello and Simone Pampanelli and {Del Sindaco}, Paola and Carlo Lalli and Marco Ciofetta and Elena Volpi and Fausto Santeusanio and Paolo Brunetti and Bolli, {Geremia B.}",
year = "1997",
language = "English (US)",
volume = "46",
pages = "1010--1016",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association Inc.",
number = "6",

}

TY - JOUR

T1 - Evidence of increased systemic glucose production and gluconeogenesis in an early stage of NIDDM

AU - Perriello, Gabriele

AU - Pampanelli, Simone

AU - Del Sindaco, Paola

AU - Lalli, Carlo

AU - Ciofetta, Marco

AU - Volpi, Elena

AU - Santeusanio, Fausto

AU - Brunetti, Paolo

AU - Bolli, Geremia B.

PY - 1997

Y1 - 1997

N2 - To assess the mechanisms of fasting hyperglycemia in NIDDM patients with mild elevation of fasting plasma glucose (FPG) compared with NIDDM patients with overt hyperglycemia, we studied 29 patients with NIDDM, who were divided in two groups according to their fasting plasma glucose (<7.8 and ≤7.8 mmol/l for groups A and B, respectively), and 16 control subjects who were matched with NIDDM patients for age, sex, and body mass index. All subjects were infused with [3-3H]glucose between 10:00 P.M. and 10:00 A.M. during overnight fasting to determine glucose fluxes. In 27 subjects (17 diabetic and 10 control), [U-14C]alanine was simultaneously infused between 4:00 A.M. and 10:00 A.M. to measure gluconeogenesis (GNG) from alanine. Arterialized-venous plasma samples were collected every 30 min for measurement of glucose fluxes, GNG, and glucoregulatory hormones. In group A, plasma glucose, rate of systemic glucose production (SGP), and GNG were greater than in control subjects (7.2 ± 0.2 vs. 4.9 ± 0.1 mmol/l, 109 ± 02 vs. 9.5 ± 03 μmol · kg-1 · min-1 and 0.58 ± 0.04 vs. 0.37 ± 0.02 μmol · kg-1 · min-1, respectively, for group A and control subjects; mean value 8:00 A.M.-10:00 A.M., all P < 0.05). Both increased SGP and GNG correlated with plasma glucose in all subjects (r = 0.77 and r = 0.75, respectively, P < 0.005). Plasma counterregulatory hormones did not differ in NIDDM patients compared to control subjects. The present studies demonstrate that SGP and GNG are increased in NIDDM patients without overt fasting hyperglycemia. Thus these metabolic abnormalities primarily contribute to early development of overnight and fasting hyperglycemia in NIDDM.

AB - To assess the mechanisms of fasting hyperglycemia in NIDDM patients with mild elevation of fasting plasma glucose (FPG) compared with NIDDM patients with overt hyperglycemia, we studied 29 patients with NIDDM, who were divided in two groups according to their fasting plasma glucose (<7.8 and ≤7.8 mmol/l for groups A and B, respectively), and 16 control subjects who were matched with NIDDM patients for age, sex, and body mass index. All subjects were infused with [3-3H]glucose between 10:00 P.M. and 10:00 A.M. during overnight fasting to determine glucose fluxes. In 27 subjects (17 diabetic and 10 control), [U-14C]alanine was simultaneously infused between 4:00 A.M. and 10:00 A.M. to measure gluconeogenesis (GNG) from alanine. Arterialized-venous plasma samples were collected every 30 min for measurement of glucose fluxes, GNG, and glucoregulatory hormones. In group A, plasma glucose, rate of systemic glucose production (SGP), and GNG were greater than in control subjects (7.2 ± 0.2 vs. 4.9 ± 0.1 mmol/l, 109 ± 02 vs. 9.5 ± 03 μmol · kg-1 · min-1 and 0.58 ± 0.04 vs. 0.37 ± 0.02 μmol · kg-1 · min-1, respectively, for group A and control subjects; mean value 8:00 A.M.-10:00 A.M., all P < 0.05). Both increased SGP and GNG correlated with plasma glucose in all subjects (r = 0.77 and r = 0.75, respectively, P < 0.005). Plasma counterregulatory hormones did not differ in NIDDM patients compared to control subjects. The present studies demonstrate that SGP and GNG are increased in NIDDM patients without overt fasting hyperglycemia. Thus these metabolic abnormalities primarily contribute to early development of overnight and fasting hyperglycemia in NIDDM.

UR - http://www.scopus.com/inward/record.url?scp=0030988702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030988702&partnerID=8YFLogxK

M3 - Article

C2 - 9166673

AN - SCOPUS:0030988702

VL - 46

SP - 1010

EP - 1016

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 6

ER -