Evidence of Pasteurella haemolytica linked immune complex disease in natural and experimental models

Jere McBride, Edward J. Wozniak, Avery W. Brewer, Diane K. Naydan, Bennie I. Osburn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The pathogenesis of bovine pneumonic pasteurellosis is not completely understood, and studies have not established that Pasteurella haemolytica A1 (Ph1) virulence is exclusively responsible for the development of acute pulmonary lesions. The purpose of this investigation was to determine if immune complex disease is involved in the pathogenesis of bovine pneumonic pasteurellosis. A retrospective immunohistologic study of lung tissue from natural cases of bovine pneumonic pasteurellosis (44) as performed, and immune complexes were observed in alveloar spaces and walls in 88% of these cases. To study this pathologic mechanism experimentally, groups of mice were immunized with purified Ph1 outer membranes (OMs) or sham immunized on days 0 and 14. Mice were challenged intratracheally on day 24 with either live Ph1 or Ph1 OMs, and pulmonary lesions were assessed 24 h after challenge. Placebo immunized mice developed focal infiltrates of neutrophils and macrophages centered around large caliber bronchi. Mice immunized with Ph1 OMs and challenged with live Ph1 or OMs developed severe bronchointerstitial pneumonia with diffuse neutrophilic infiltration, focal necrosis, hemorrhage and edema, that is histologically similar to bovine pneumonic pasteurellosis. Immunohistology revealed flocculent aggregates of IgG and complement positive material within alveolar spaces and walls from mice challenged with live Ph1, and fine granular deposits of IgG and complement positive material were observed lining the alveolar walls from mice challenged with Ph1 OMs. Immunized mice exhibited high serum IgG antibody titers to Ph1 outer membrane proteins (OMPs). Results of this study suggest that immune complex disease plays a role in the pathogenesis of bovine pneumonic pasteurellosis.

Original languageEnglish (US)
Pages (from-to)183-193
Number of pages11
JournalMicrobial Pathogenesis
Volume26
Issue number4
DOIs
StatePublished - Apr 1999

Fingerprint

Immune Complex Diseases
Mannheimia haemolytica
Pneumonic Pasteurellosis
Theoretical Models
Membranes
Immunoglobulin G
Lung
Bronchi
Antigen-Antibody Complex
Virulence
Edema
Pneumonia
Membrane Proteins
Neutrophils
Necrosis
Retrospective Studies
Macrophages
Placebos
Hemorrhage

Keywords

  • Antibody
  • Hypersensitivity
  • Immune complex
  • Pasteurella haemolytica
  • Pasteurellosis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Evidence of Pasteurella haemolytica linked immune complex disease in natural and experimental models. / McBride, Jere; Wozniak, Edward J.; Brewer, Avery W.; Naydan, Diane K.; Osburn, Bennie I.

In: Microbial Pathogenesis, Vol. 26, No. 4, 04.1999, p. 183-193.

Research output: Contribution to journalArticle

McBride, Jere ; Wozniak, Edward J. ; Brewer, Avery W. ; Naydan, Diane K. ; Osburn, Bennie I. / Evidence of Pasteurella haemolytica linked immune complex disease in natural and experimental models. In: Microbial Pathogenesis. 1999 ; Vol. 26, No. 4. pp. 183-193.
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