Evidence of sexual reproduction in the protozoan parasite Leishmania (kinetoplastida

Trypanosomatidae)

R. D. Kreutzer, J. J. Yemma, M. Grogl, R. B. Tesh, T. I. Martin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Parasitic protozoa of the genus Leishmania (Kinetoplastida: Trypanosomatidae) are generally thought to multiply by binary fission; however, data from quantitative microspectrophotometry indicate that nuclear fusion or sexual reproduction takes place in the intracellular amastigote form. Among several different Leishmania species, the mean ± SD nuclear DNA content of all promastigotes (extracellular form) and of some amastigotes (intracellular form) in macrophages was 0.098 ± 0.032 relative units; in contrast, other amastigotes within the same macrophage had a mean ± SD nuclear DNA content of 0.219 ± 0.050. The latter population of amastigotes are apparently produced when the nuclei of a pair of 0.098 amastigotes fuse. These 0.219 amastigotes later go through what is probably the typical meiotic cycle to reform the 0.098 condition we observed among promastigotes. The demonstration of sexual reproduction in Leishmania has important implications for the future direction of research on this medically important parasite.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume51
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Kinetoplastida
Trypanosomatina
Leishmania
Reproduction
Parasites
Nuclear Fusion
Macrophages
Microspectrophotometry
DNA
Research
Population

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Evidence of sexual reproduction in the protozoan parasite Leishmania (kinetoplastida : Trypanosomatidae). / Kreutzer, R. D.; Yemma, J. J.; Grogl, M.; Tesh, R. B.; Martin, T. I.

In: American Journal of Tropical Medicine and Hygiene, Vol. 51, No. 3, 1994, p. 301-307.

Research output: Contribution to journalArticle

Kreutzer, R. D. ; Yemma, J. J. ; Grogl, M. ; Tesh, R. B. ; Martin, T. I. / Evidence of sexual reproduction in the protozoan parasite Leishmania (kinetoplastida : Trypanosomatidae). In: American Journal of Tropical Medicine and Hygiene. 1994 ; Vol. 51, No. 3. pp. 301-307.
@article{f2f6905b10804b64a65949c3503f7b5f,
title = "Evidence of sexual reproduction in the protozoan parasite Leishmania (kinetoplastida: Trypanosomatidae)",
abstract = "Parasitic protozoa of the genus Leishmania (Kinetoplastida: Trypanosomatidae) are generally thought to multiply by binary fission; however, data from quantitative microspectrophotometry indicate that nuclear fusion or sexual reproduction takes place in the intracellular amastigote form. Among several different Leishmania species, the mean ± SD nuclear DNA content of all promastigotes (extracellular form) and of some amastigotes (intracellular form) in macrophages was 0.098 ± 0.032 relative units; in contrast, other amastigotes within the same macrophage had a mean ± SD nuclear DNA content of 0.219 ± 0.050. The latter population of amastigotes are apparently produced when the nuclei of a pair of 0.098 amastigotes fuse. These 0.219 amastigotes later go through what is probably the typical meiotic cycle to reform the 0.098 condition we observed among promastigotes. The demonstration of sexual reproduction in Leishmania has important implications for the future direction of research on this medically important parasite.",
author = "Kreutzer, {R. D.} and Yemma, {J. J.} and M. Grogl and Tesh, {R. B.} and Martin, {T. I.}",
year = "1994",
language = "English (US)",
volume = "51",
pages = "301--307",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

TY - JOUR

T1 - Evidence of sexual reproduction in the protozoan parasite Leishmania (kinetoplastida

T2 - Trypanosomatidae)

AU - Kreutzer, R. D.

AU - Yemma, J. J.

AU - Grogl, M.

AU - Tesh, R. B.

AU - Martin, T. I.

PY - 1994

Y1 - 1994

N2 - Parasitic protozoa of the genus Leishmania (Kinetoplastida: Trypanosomatidae) are generally thought to multiply by binary fission; however, data from quantitative microspectrophotometry indicate that nuclear fusion or sexual reproduction takes place in the intracellular amastigote form. Among several different Leishmania species, the mean ± SD nuclear DNA content of all promastigotes (extracellular form) and of some amastigotes (intracellular form) in macrophages was 0.098 ± 0.032 relative units; in contrast, other amastigotes within the same macrophage had a mean ± SD nuclear DNA content of 0.219 ± 0.050. The latter population of amastigotes are apparently produced when the nuclei of a pair of 0.098 amastigotes fuse. These 0.219 amastigotes later go through what is probably the typical meiotic cycle to reform the 0.098 condition we observed among promastigotes. The demonstration of sexual reproduction in Leishmania has important implications for the future direction of research on this medically important parasite.

AB - Parasitic protozoa of the genus Leishmania (Kinetoplastida: Trypanosomatidae) are generally thought to multiply by binary fission; however, data from quantitative microspectrophotometry indicate that nuclear fusion or sexual reproduction takes place in the intracellular amastigote form. Among several different Leishmania species, the mean ± SD nuclear DNA content of all promastigotes (extracellular form) and of some amastigotes (intracellular form) in macrophages was 0.098 ± 0.032 relative units; in contrast, other amastigotes within the same macrophage had a mean ± SD nuclear DNA content of 0.219 ± 0.050. The latter population of amastigotes are apparently produced when the nuclei of a pair of 0.098 amastigotes fuse. These 0.219 amastigotes later go through what is probably the typical meiotic cycle to reform the 0.098 condition we observed among promastigotes. The demonstration of sexual reproduction in Leishmania has important implications for the future direction of research on this medically important parasite.

UR - http://www.scopus.com/inward/record.url?scp=0028061173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028061173&partnerID=8YFLogxK

M3 - Article

VL - 51

SP - 301

EP - 307

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 3

ER -