Evolution of alphaviruses in the eastern equine encephalomyelitis complex

Scott Weaver, Amy Hagenbaugh, Liz Anne Bellew, Laurent Gousset, Varuni Mallampalli, John J. Holland, Thomas W. Scott

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Evolution of viruses in the eastern equine encephalomyelitis (EEE) complex was studied by analyzing RNA sequences and oligonucleotide fingerprints from isolates representing the North and South American antigenic varieties. By using homologous sequences of Venezuelan equine encephalomyelitis virus as an outgroup, phylogenetic trees revealed three main EEE virus monophyletic groups. A North American variety group included all isolates from North America and the Caribbean. One South American variety group included isolates from the Amazon basin in Brazil and Peru, while the other included strains from Argentina, Guyana, Ecuador, Panama, Trinidad, and Venezuela. No evidence of heterologous recombination was obtained when three separate regions of the EEE virus genome were analyzed independently. Estimates of the overall rate of EEE virus evolution (nucleotide substitution) were 1.6 × 10-4 substitution per nucleotide per year for the North American group and 4.3 × 10-4 for the Argentina-Panama South American group. Evolutionary rate estimates for the North American group increased over 10-fold (from about 2 × 10-5 to 4 × 10-4) concurrent with divergence of two monophyletic groups during the early 1970s. The North and South American antigenic varieties diverged roughly 1,000 years ago, while the two main South American groups diverged about 450 years ago. Analysis of multiple strains isolated from an upstate New York transmission focus during the same years suggested that, in certain locations, EEE virus may be relatively isolated for short time periods.

Original languageEnglish (US)
Pages (from-to)158-169
Number of pages12
JournalJournal of Virology
Volume68
Issue number1
StatePublished - Jan 1994
Externally publishedYes

Fingerprint

Eastern Equine Encephalomyelitis
Eastern equine encephalomyelitis
Eastern equine encephalitis virus
Alphavirus
Panama
Argentina
Nucleotides
nucleotides
Guyana
Venezuelan Equine Encephalitis Viruses
Venezuelan equine encephalitis virus
Trinidad and Tobago
Ecuador
Venezuela
Peru
Dermatoglyphics
Sequence Homology
North America
oligonucleotides
sequence homology

ASJC Scopus subject areas

  • Immunology

Cite this

Weaver, S., Hagenbaugh, A., Bellew, L. A., Gousset, L., Mallampalli, V., Holland, J. J., & Scott, T. W. (1994). Evolution of alphaviruses in the eastern equine encephalomyelitis complex. Journal of Virology, 68(1), 158-169.

Evolution of alphaviruses in the eastern equine encephalomyelitis complex. / Weaver, Scott; Hagenbaugh, Amy; Bellew, Liz Anne; Gousset, Laurent; Mallampalli, Varuni; Holland, John J.; Scott, Thomas W.

In: Journal of Virology, Vol. 68, No. 1, 01.1994, p. 158-169.

Research output: Contribution to journalArticle

Weaver, S, Hagenbaugh, A, Bellew, LA, Gousset, L, Mallampalli, V, Holland, JJ & Scott, TW 1994, 'Evolution of alphaviruses in the eastern equine encephalomyelitis complex', Journal of Virology, vol. 68, no. 1, pp. 158-169.
Weaver S, Hagenbaugh A, Bellew LA, Gousset L, Mallampalli V, Holland JJ et al. Evolution of alphaviruses in the eastern equine encephalomyelitis complex. Journal of Virology. 1994 Jan;68(1):158-169.
Weaver, Scott ; Hagenbaugh, Amy ; Bellew, Liz Anne ; Gousset, Laurent ; Mallampalli, Varuni ; Holland, John J. ; Scott, Thomas W. / Evolution of alphaviruses in the eastern equine encephalomyelitis complex. In: Journal of Virology. 1994 ; Vol. 68, No. 1. pp. 158-169.
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