Evolution on the Biophysical Fitness Landscape of an RNA Virus

Assaf Rotem, Adrian W.R. Serohijos, Connie B. Chang, Joshua T. Wolfe, Audrey E. Fischer, Thomas S. Mehoke, Huidan Zhang, Ye Tao, W. Lloyd Ung, Jeong Mo Choi, João V. Rodrigues, Abimbola O. Kolawole, Stephan A. Koehler, Susan Wu, Peter M. Thielen, Naiwen Cui, Plamen A. Demirev, Nicholas S. Giacobbi, Timothy R. Julian, Kellogg SchwabJeffrey S. Lin, Thomas Smith, James M. Pipas, Christiane E. Wobus, Andrew B. Feldman, David A. Weitz, Eugene I. Shakhnovich

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Viral evolutionary pathways are determined by the fitness landscape, which maps viral genotype to fitness. However, a quantitative description of the landscape and the evolutionary forces on it remain elusive. Here, we apply a biophysical fitness model based on capsid folding stability and antibody binding affinity to predict the evolutionary pathway of norovirus escaping a neutralizing antibody. The model is validated by experimental evolution in bulk culture and in a drop-based microfluidics that propagates millions of independent small viral subpopulations. We demonstrate that along the axis of binding affinity, selection for escape variants and drift due to random mutations have the same direction, an atypical case in evolution. However, along folding stability, selection and drift are opposing forces whose balance is tuned by viral population size. Our results demonstrate that predictable epistatic tradeoffs between molecular traits of viral proteins shape viral evolution.

Original languageEnglish (US)
Pages (from-to)2390-2400
Number of pages11
JournalMolecular Biology and Evolution
Volume35
Issue number10
DOIs
StatePublished - Oct 1 2018

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ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Rotem, A., Serohijos, A. W. R., Chang, C. B., Wolfe, J. T., Fischer, A. E., Mehoke, T. S., Zhang, H., Tao, Y., Lloyd Ung, W., Choi, J. M., Rodrigues, J. V., Kolawole, A. O., Koehler, S. A., Wu, S., Thielen, P. M., Cui, N., Demirev, P. A., Giacobbi, N. S., Julian, T. R., ... Shakhnovich, E. I. (2018). Evolution on the Biophysical Fitness Landscape of an RNA Virus. Molecular Biology and Evolution, 35(10), 2390-2400. https://doi.org/10.1093/molbev/msy131