Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species

Kan Fujino, Masayuki Horie, Tomoyuki Honda, Shoko Nakamura, Yusuke Matsumoto, Ivo M B Francischetti, Keizo Tomonaga

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bornavirus, a non-segmented, negative-strand RNA viruses, is currently classified into several genetically distinct genotypes, such as Borna disease virus (BDV) and avian bornaviruses (ABVs). Recent studies revealed that bornavirus genotypes show unique sequence variability in the putative 5′ untranslated region (5′ UTR) of X/P mRNA, a bicistronic mRNA for the X protein and phosphoprotein (P). In this study, to understand the evolutionary relationship among the bornavirus genotypes, we investigated the functional interaction between the X and P proteins of four bornavirus genotypes, BDV, ABV genotype 4 and 5 and reptile bornavirus (RBV), the putative 5′ UTRs of which exhibit variation in the length. Immunofluorescence and immunoprecipitation analyses using mammalian and avian cell lines revealed that the X proteins of bornaviruses conserve the ability to facilitate the export of P from the nucleus to the cytoplasm via interaction with P. Furthermore, we showed that inter-genotypic interactions may occur between X and P among the genotypes, except for X of RBV. In addition, a BDV minireplicon assay demonstrated that the X and P proteins of ABVs, but not RBV, can affect the polymerase activity of BDV. This study demonstrates that bornaviruses may have conserved the fundamental function of a regulatory protein during their evolution, whereas RBV has evolved distinctly from the other bornavirus genotypes.

Original languageEnglish (US)
Article numbere51161
JournalPLoS One
Volume7
Issue number12
DOIs
StatePublished - Dec 7 2012
Externally publishedYes

Fingerprint

Bornaviridae
Bornavirus
phosphoproteins
Phosphoproteins
Viruses
Vertebrates
vertebrates
5' Untranslated Regions
Borna disease virus
Proteins
proteins
Genotype
Reptiles
Avian Proteins
genotype
reptiles
Messenger RNA
Assays
5' untranslated regions
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fujino, K., Horie, M., Honda, T., Nakamura, S., Matsumoto, Y., Francischetti, I. M. B., & Tomonaga, K. (2012). Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species. PLoS One, 7(12), [e51161]. https://doi.org/10.1371/journal.pone.0051161

Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species. / Fujino, Kan; Horie, Masayuki; Honda, Tomoyuki; Nakamura, Shoko; Matsumoto, Yusuke; Francischetti, Ivo M B; Tomonaga, Keizo.

In: PLoS One, Vol. 7, No. 12, e51161, 07.12.2012.

Research output: Contribution to journalArticle

Fujino, K, Horie, M, Honda, T, Nakamura, S, Matsumoto, Y, Francischetti, IMB & Tomonaga, K 2012, 'Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species', PLoS One, vol. 7, no. 12, e51161. https://doi.org/10.1371/journal.pone.0051161
Fujino, Kan ; Horie, Masayuki ; Honda, Tomoyuki ; Nakamura, Shoko ; Matsumoto, Yusuke ; Francischetti, Ivo M B ; Tomonaga, Keizo. / Evolutionarily Conserved Interaction between the Phosphoproteins and X Proteins of Bornaviruses from Different Vertebrate Species. In: PLoS One. 2012 ; Vol. 7, No. 12.
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