Exact mass measurement: How petroleum crude oil analysis can help biochemists

Alan G. Marshall, Greg T. Blakney, Mark R. Emmett, Christopher L. Hendrickson, Ryan P. Rodgers

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Petroleum crude oil is nature's most complex organic mixture, exhibiting >10,000 different elemental compositions (not counting isomers) within a dynamic range of 10,000:1 in a single mass spectrum. Petroleum provides an excellent template for improving mass resolution and mass accuracy for other applications. For example, the same mass isobars (i.e., two compounds of different chemical formula but the same nominal (nearest integer) mass appear in both petroleum and proteins (e.g., SH4 vs. C3, differing by 0.036 Da). Recently, we have achieved broadband "phasing" of Fourier transform ion cyclotron resonance mass spectra, based on resolution of multiple isobars at each nominal mass in a petroleum spectrum. Moreover, small systematic mass measurement errors with periodicity less than 1 Da become observable in petroleum FT-ICR mass spectra, for potential five-fold reduction in baseline noise. Several biological applications will be described. Supported by NSF DMR-0654118, NIH R01 GM78359 and the State of Florida.

Original languageEnglish (US)
Title of host publicationAmerican Chemical Society - 238th National Meeting and Exposition, ACS 2009, Abstracts of Scientific Papers
StatePublished - 2009
Externally publishedYes
Event238th National Meeting and Exposition of the American Chemical Society, ACS 2009 - Washington, DC, United States
Duration: Aug 16 2009Aug 20 2009

Publication series

NameACS National Meeting Book of Abstracts
ISSN (Print)0065-7727

Other

Other238th National Meeting and Exposition of the American Chemical Society, ACS 2009
Country/TerritoryUnited States
CityWashington, DC
Period8/16/098/20/09

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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