Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

Alan Barrett, A. Pryde, A. R. Medlen, T. N. Ledger, J. E. Whitby, C. A. Gibson, M. DeSilva, D. J. Groves, D. J. Langley, P. D. Minor

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalVaccine
Volume7
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Yellow Fever Vaccine
Yellow fever virus
glycoproteins
monoclonal antibodies
Glycoproteins
Monoclonal Antibodies
vaccines
Neutralization Tests
viruses
Viruses
neutralization tests
Vaccines
Avian Leukosis Virus
Avian leukosis virus
Invertebrates
Neutralizing Antibodies
Brazil
Vertebrates
World Health Organization
Epitopes

Keywords

  • flavivirus
  • monoclonal antibodies
  • Yellow fever

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Barrett, A., Pryde, A., Medlen, A. R., Ledger, T. N., Whitby, J. E., Gibson, C. A., ... Minor, P. D. (1989). Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies. Vaccine, 7(4), 333-336. https://doi.org/10.1016/0264-410X(89)90196-5

Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies. / Barrett, Alan; Pryde, A.; Medlen, A. R.; Ledger, T. N.; Whitby, J. E.; Gibson, C. A.; DeSilva, M.; Groves, D. J.; Langley, D. J.; Minor, P. D.

In: Vaccine, Vol. 7, No. 4, 1989, p. 333-336.

Research output: Contribution to journalArticle

Barrett, A, Pryde, A, Medlen, AR, Ledger, TN, Whitby, JE, Gibson, CA, DeSilva, M, Groves, DJ, Langley, DJ & Minor, PD 1989, 'Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies', Vaccine, vol. 7, no. 4, pp. 333-336. https://doi.org/10.1016/0264-410X(89)90196-5
Barrett, Alan ; Pryde, A. ; Medlen, A. R. ; Ledger, T. N. ; Whitby, J. E. ; Gibson, C. A. ; DeSilva, M. ; Groves, D. J. ; Langley, D. J. ; Minor, P. D. / Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies. In: Vaccine. 1989 ; Vol. 7, No. 4. pp. 333-336.
@article{66aa30095ef54ff99a329eb40303282b,
title = "Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies",
abstract = "Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.",
keywords = "flavivirus, monoclonal antibodies, Yellow fever",
author = "Alan Barrett and A. Pryde and Medlen, {A. R.} and Ledger, {T. N.} and Whitby, {J. E.} and Gibson, {C. A.} and M. DeSilva and Groves, {D. J.} and Langley, {D. J.} and Minor, {P. D.}",
year = "1989",
doi = "10.1016/0264-410X(89)90196-5",
language = "English (US)",
volume = "7",
pages = "333--336",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

AU - Barrett, Alan

AU - Pryde, A.

AU - Medlen, A. R.

AU - Ledger, T. N.

AU - Whitby, J. E.

AU - Gibson, C. A.

AU - DeSilva, M.

AU - Groves, D. J.

AU - Langley, D. J.

AU - Minor, P. D.

PY - 1989

Y1 - 1989

N2 - Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.

AB - Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.

KW - flavivirus

KW - monoclonal antibodies

KW - Yellow fever

UR - http://www.scopus.com/inward/record.url?scp=0024416690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024416690&partnerID=8YFLogxK

U2 - 10.1016/0264-410X(89)90196-5

DO - 10.1016/0264-410X(89)90196-5

M3 - Article

VL - 7

SP - 333

EP - 336

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 4

ER -