Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies

Alan Barrett, A. Pryde, A. R. Medlen, T. N. Ledger, J. E. Whitby, C. A. Gibson, M. DeSilva, D. J. Groves, D. J. Langley, P. D. Minor

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Two panels of envelope glycoprotein reactive monoclonal antibodies (mAbs) were prepared against yellow fever (YF) 17D vaccine viruses. Five mAbs were prepared against the World Health Organization 17D-204 avian leukosis virus-free secondary seed virus and eight mAbs against 17DD vaccine manufactured in Brazil. The majority of these mAbs were type-specific and displayed differing reactions in neutralization tests. One, B14, would only neutralize YF vaccine virus grown in invertebrate cells. Others would differentiate 17D-204 and 17DD vaccines, from different manufacturers, in neutralization tests when the viruses were grown in vertebrate cells. The data indicate that heterogeneity exists between the epitopes that elicit neutralizing antibody on YF vaccine from different manufacturers.

Original languageEnglish (US)
Pages (from-to)333-336
Number of pages4
JournalVaccine
Volume7
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

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Keywords

  • flavivirus
  • monoclonal antibodies
  • Yellow fever

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Barrett, A., Pryde, A., Medlen, A. R., Ledger, T. N., Whitby, J. E., Gibson, C. A., DeSilva, M., Groves, D. J., Langley, D. J., & Minor, P. D. (1989). Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies. Vaccine, 7(4), 333-336. https://doi.org/10.1016/0264-410X(89)90196-5