Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury

Ryan M. Huebinger, Ming Mei Liu, Scot E. Dowd, Fernando A. Rivera-Chavez, John Boynton, Curtis Carey, Kenneth Hawkins, Christian T. Minshall, Steven Wolf, Joseph P. Minei, Robert C. Barber

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: We examined the microbiota of bronchoalveolar lavage (BAL) samples with next-generation sequencing (NGS) technology to determine whether its results correlate with those of standard culture methods or affect patient outcome or both. Methods: We collected BAL samples in the surgical intensive care unit (SICU) as part of the standard of care for intubated individuals who had a Clinical Pulmonary Infection Score (CPIS) ≥6 points. A portion of the BAL fluid was sequenced for the 16S region of ribosomal deoxyribonucleic acid (rDNA) with the Roche 454 FLX Titanium sequencer. Sequences were analyzed through a data-analysis pipeline to identify the appropriate taxonomic designation (∼species) of each 16s sequence. The bacterial microbiota of each BAL sample was compared with the bacteria identified in the sample through standard culture methods. Correlations between the taxonomic diversity of the microbiota and clinical outcome were examined through linear regression and Pearson correlation. Results: Bronchoalveolar lavage samples from 12 individuals in the SICU who had a CPIS ≥6 points were examined through 454 pyrosequencing. The number of phylotypes (∼species) in the samples ranged from 15 to 129. The number of phyla in the BAL samples ranged from 3 to 14. There was little correlation between the bacteria identified by NGS and those identified with standard culture methods. The same predominant bacterial strain was identified by both culture and sequencing in only a single sample. The correlation between patient days on a ventilator and the number of species in BAL samples was significant (r=0.7435, p=0.0056; r2=0.5528). Conclusions: Increasing diversity of the bacterial microbiota in BAL samples correlates with the duration of mechanical ventilation. Bacteria identified through standard culture methods were not well correlated with the findings of NGS.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalSurgical Infections
Volume14
Issue number3
DOIs
StatePublished - Jun 1 2013
Externally publishedYes

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Microbiota
Bronchoalveolar Lavage
Technology
Wounds and Injuries
Critical Care
Bacteria
Intensive Care Units
Lung
Bronchoalveolar Lavage Fluid
Mechanical Ventilators
Standard of Care
Infection
Titanium
Artificial Respiration
Linear Models
DNA

ASJC Scopus subject areas

  • Surgery
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Huebinger, R. M., Liu, M. M., Dowd, S. E., Rivera-Chavez, F. A., Boynton, J., Carey, C., ... Barber, R. C. (2013). Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury. Surgical Infections, 14(3), 275-282. https://doi.org/10.1089/sur.2012.095

Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury. / Huebinger, Ryan M.; Liu, Ming Mei; Dowd, Scot E.; Rivera-Chavez, Fernando A.; Boynton, John; Carey, Curtis; Hawkins, Kenneth; Minshall, Christian T.; Wolf, Steven; Minei, Joseph P.; Barber, Robert C.

In: Surgical Infections, Vol. 14, No. 3, 01.06.2013, p. 275-282.

Research output: Contribution to journalArticle

Huebinger, RM, Liu, MM, Dowd, SE, Rivera-Chavez, FA, Boynton, J, Carey, C, Hawkins, K, Minshall, CT, Wolf, S, Minei, JP & Barber, RC 2013, 'Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury', Surgical Infections, vol. 14, no. 3, pp. 275-282. https://doi.org/10.1089/sur.2012.095
Huebinger, Ryan M. ; Liu, Ming Mei ; Dowd, Scot E. ; Rivera-Chavez, Fernando A. ; Boynton, John ; Carey, Curtis ; Hawkins, Kenneth ; Minshall, Christian T. ; Wolf, Steven ; Minei, Joseph P. ; Barber, Robert C. / Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury. In: Surgical Infections. 2013 ; Vol. 14, No. 3. pp. 275-282.
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AU - Boynton, John

AU - Carey, Curtis

AU - Hawkins, Kenneth

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