Examining troughs in the mass distribution of all theoretically possible tryptic peptides

Alexey V. Nefedov, Indranil Mitra, Allan R. Brasier, Rovshan G. Sadygov

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

This work describes the mass distribution of all theoretically possibly tryptic peptides made of 20 amino acids, up to the mass of 3 kDa, with resolution of 0.001 Da. We characterize regions between the peaks of the distribution, including gaps (forbidden zones) and low-populated areas (quiet zones). We show how the gaps shrink over the mass range and when they completely disappear. We demonstrate that peptide compositions in quiet zones are less diverse than those in the peaks of the distribution and that by eliminating certain types of unrealistic compositions the gaps in the distribution may be increased. The mass distribution is generated using a parallel implementation of a recursive procedure that enumerates all amino acid compositions. It allows us to enumerate all compositions of tryptic peptides below 3 kDa in 48 min using a computer cluster with 12 Intel Xeon X5650 CPUs (72 cores). The results of this work can be used to facilitate protein identification and mass defect labeling in mass spectrometry-based proteomics experiments.

Original languageEnglish (US)
Pages (from-to)4150-4157
Number of pages8
JournalJournal of Proteome Research
Volume10
Issue number9
DOIs
StatePublished - Sep 2 2011

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Keywords

  • accurate peptide masses
  • amino acid compositions of all theoretically possible peptides
  • distribution of peptide masses
  • forbidden zones
  • mass accuracy
  • quiet zones

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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