Exercise Altered the Skeletal Muscle MicroRNAs and Gene Expression Profiles in Burn Rats with Hindlimb Unloading

Juquan Song, Melody R. Saeman, Lisa A. Baer, Anthony R. Cai, Charles E. Wade, Steven Wolf

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This study investigated microRNA and target gene profiles under different conditions of burn, bed rest, and exercise training. Male Sprague-Dawley rats (n = 48) were assigned to sham ambulatory, sham hindlimb unloading, burn ambulatory, or burn plus hindlimb unloading groups. Rats received a 40% TBSA scald burn or sham treatments and were ambulatory or hindlimb unloaded. Rats were further assigned to exercise or no exercise. Plantaris tissues were harvested on day 14 and pooled to analyze for microRNA and gene expression profiles. Compared with the sham ambulatory-no exercise group, 73, 79, and 80 microRNAs were altered 2-fold in the burn ambulatory, sham hindlimb unloading, and burn hindlimb unloading groups, all with no exercise, respectively. More than 70% of microRNAs were upregulated in response to burn and hindlimb unloading, whereas 60% microRNA of the profile decreased in hindlimb unloaded burn rats with exercise training. MiR-182 was the most affected in rat muscle. Gene ontology biological process and pathway analysis showed that the oxidative stress pathway was most stimulated in the hindlimb unloaded burn rats; while in response to exercise training, all genes in related pathways such as hypermetabolic, inflammation, and blood coagulation were alleviated. MicroRNAs and transcript gene profiles were altered in burn and hindlimb unloading groups, with additive effects on hindlimb unloaded burn rats. The altered genes' signal pathways were associated with muscle mass loss and function impairment. Muscle improvement with exercise training was observed in gene levels with microRNA alterations as well.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalJournal of Burn Care and Research
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Fingerprint

Hindlimb Suspension
MicroRNAs
Transcriptome
Skeletal Muscle
Hindlimb
Exercise
Genes
Muscles
Biological Phenomena
Bed Rest
Gene Ontology
Blood Coagulation
Sprague Dawley Rats
Signal Transduction
Oxidative Stress
Placebos
Inflammation

ASJC Scopus subject areas

  • Surgery
  • Emergency Medicine
  • Rehabilitation

Cite this

Exercise Altered the Skeletal Muscle MicroRNAs and Gene Expression Profiles in Burn Rats with Hindlimb Unloading. / Song, Juquan; Saeman, Melody R.; Baer, Lisa A.; Cai, Anthony R.; Wade, Charles E.; Wolf, Steven.

In: Journal of Burn Care and Research, Vol. 38, No. 1, 01.01.2017, p. 11-19.

Research output: Contribution to journalArticle

Song, Juquan ; Saeman, Melody R. ; Baer, Lisa A. ; Cai, Anthony R. ; Wade, Charles E. ; Wolf, Steven. / Exercise Altered the Skeletal Muscle MicroRNAs and Gene Expression Profiles in Burn Rats with Hindlimb Unloading. In: Journal of Burn Care and Research. 2017 ; Vol. 38, No. 1. pp. 11-19.
@article{1beedb3a37b64452bdfe78222c39f496,
title = "Exercise Altered the Skeletal Muscle MicroRNAs and Gene Expression Profiles in Burn Rats with Hindlimb Unloading",
abstract = "This study investigated microRNA and target gene profiles under different conditions of burn, bed rest, and exercise training. Male Sprague-Dawley rats (n = 48) were assigned to sham ambulatory, sham hindlimb unloading, burn ambulatory, or burn plus hindlimb unloading groups. Rats received a 40{\%} TBSA scald burn or sham treatments and were ambulatory or hindlimb unloaded. Rats were further assigned to exercise or no exercise. Plantaris tissues were harvested on day 14 and pooled to analyze for microRNA and gene expression profiles. Compared with the sham ambulatory-no exercise group, 73, 79, and 80 microRNAs were altered 2-fold in the burn ambulatory, sham hindlimb unloading, and burn hindlimb unloading groups, all with no exercise, respectively. More than 70{\%} of microRNAs were upregulated in response to burn and hindlimb unloading, whereas 60{\%} microRNA of the profile decreased in hindlimb unloaded burn rats with exercise training. MiR-182 was the most affected in rat muscle. Gene ontology biological process and pathway analysis showed that the oxidative stress pathway was most stimulated in the hindlimb unloaded burn rats; while in response to exercise training, all genes in related pathways such as hypermetabolic, inflammation, and blood coagulation were alleviated. MicroRNAs and transcript gene profiles were altered in burn and hindlimb unloading groups, with additive effects on hindlimb unloaded burn rats. The altered genes' signal pathways were associated with muscle mass loss and function impairment. Muscle improvement with exercise training was observed in gene levels with microRNA alterations as well.",
author = "Juquan Song and Saeman, {Melody R.} and Baer, {Lisa A.} and Cai, {Anthony R.} and Wade, {Charles E.} and Steven Wolf",
year = "2017",
month = "1",
day = "1",
doi = "10.1097/BCR.0000000000000444",
language = "English (US)",
volume = "38",
pages = "11--19",
journal = "Journal of Burn Care and Research",
issn = "1559-047X",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Exercise Altered the Skeletal Muscle MicroRNAs and Gene Expression Profiles in Burn Rats with Hindlimb Unloading

AU - Song, Juquan

AU - Saeman, Melody R.

AU - Baer, Lisa A.

AU - Cai, Anthony R.

AU - Wade, Charles E.

AU - Wolf, Steven

PY - 2017/1/1

Y1 - 2017/1/1

N2 - This study investigated microRNA and target gene profiles under different conditions of burn, bed rest, and exercise training. Male Sprague-Dawley rats (n = 48) were assigned to sham ambulatory, sham hindlimb unloading, burn ambulatory, or burn plus hindlimb unloading groups. Rats received a 40% TBSA scald burn or sham treatments and were ambulatory or hindlimb unloaded. Rats were further assigned to exercise or no exercise. Plantaris tissues were harvested on day 14 and pooled to analyze for microRNA and gene expression profiles. Compared with the sham ambulatory-no exercise group, 73, 79, and 80 microRNAs were altered 2-fold in the burn ambulatory, sham hindlimb unloading, and burn hindlimb unloading groups, all with no exercise, respectively. More than 70% of microRNAs were upregulated in response to burn and hindlimb unloading, whereas 60% microRNA of the profile decreased in hindlimb unloaded burn rats with exercise training. MiR-182 was the most affected in rat muscle. Gene ontology biological process and pathway analysis showed that the oxidative stress pathway was most stimulated in the hindlimb unloaded burn rats; while in response to exercise training, all genes in related pathways such as hypermetabolic, inflammation, and blood coagulation were alleviated. MicroRNAs and transcript gene profiles were altered in burn and hindlimb unloading groups, with additive effects on hindlimb unloaded burn rats. The altered genes' signal pathways were associated with muscle mass loss and function impairment. Muscle improvement with exercise training was observed in gene levels with microRNA alterations as well.

AB - This study investigated microRNA and target gene profiles under different conditions of burn, bed rest, and exercise training. Male Sprague-Dawley rats (n = 48) were assigned to sham ambulatory, sham hindlimb unloading, burn ambulatory, or burn plus hindlimb unloading groups. Rats received a 40% TBSA scald burn or sham treatments and were ambulatory or hindlimb unloaded. Rats were further assigned to exercise or no exercise. Plantaris tissues were harvested on day 14 and pooled to analyze for microRNA and gene expression profiles. Compared with the sham ambulatory-no exercise group, 73, 79, and 80 microRNAs were altered 2-fold in the burn ambulatory, sham hindlimb unloading, and burn hindlimb unloading groups, all with no exercise, respectively. More than 70% of microRNAs were upregulated in response to burn and hindlimb unloading, whereas 60% microRNA of the profile decreased in hindlimb unloaded burn rats with exercise training. MiR-182 was the most affected in rat muscle. Gene ontology biological process and pathway analysis showed that the oxidative stress pathway was most stimulated in the hindlimb unloaded burn rats; while in response to exercise training, all genes in related pathways such as hypermetabolic, inflammation, and blood coagulation were alleviated. MicroRNAs and transcript gene profiles were altered in burn and hindlimb unloading groups, with additive effects on hindlimb unloaded burn rats. The altered genes' signal pathways were associated with muscle mass loss and function impairment. Muscle improvement with exercise training was observed in gene levels with microRNA alterations as well.

UR - http://www.scopus.com/inward/record.url?scp=84991497929&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991497929&partnerID=8YFLogxK

U2 - 10.1097/BCR.0000000000000444

DO - 10.1097/BCR.0000000000000444

M3 - Article

C2 - 27753701

AN - SCOPUS:84991497929

VL - 38

SP - 11

EP - 19

JO - Journal of Burn Care and Research

JF - Journal of Burn Care and Research

SN - 1559-047X

IS - 1

ER -