Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats

Erika Koltai, Zsofia Szabo, Mustafa Atalay, Istvan Boldogh, Hisashi Naito, Sataro Goto, Csaba Nyakas, Zsolt Radak

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Silent information regulators are potent NAD+-dependent protein deacetylases, which have been shown to regulate gene silencing, muscle differentiation and DNA damage repair. Here, changes in the level and activity of sirtuin 1 (SIRT1) in response to exercise in groups of young and old rats were studied. There was an age-related increase in SIRT1 level, while exercise training significantly increased the relative activity of SIRT1. A strong inverse correlation was found between the nuclear activity of SIRT1 and the level of acetylated proteins. Exercise training induced SIRT1 activity due to the positive effect of exercise on the activity of nicotinamide phosphoribosyltransferase (NAMPT) and thereby the production of sirtuin-fueling NAD+. Exercise training normalized the age-associated shift in redox balance, since exercised animals had significantly lower levels of carbonylated proteins, expression of hypoxia-inducible factor-1alpha and vascular endothelial growth factor. The age-associated increase in the level of SIRT6 was attenuated by exercise training. On the other hand, aging did not significantly increase the level of DNA damage, which was in line with the activity of 8-oxoguanine DNA glycosylase, while exercise training increased the level of this enzyme. Regular exercise decelerates the deleterious effects of the aging process via SIRT1-dependent pathways through the stimulation of NAD+ biosynthesis by NAMPT.

Original languageEnglish (US)
Pages (from-to)21-28
Number of pages8
JournalMechanisms of Ageing and Development
Volume131
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

Sirtuin 1
Nicotinamide Phosphoribosyltransferase
NAD
Skeletal Muscle
Exercise
DNA Damage
DNA Glycosylases
Proteins
Gene Silencing
DNA Repair
Vascular Endothelial Growth Factor A
Oxidation-Reduction
Muscles
Enzymes

Keywords

  • Aging
  • DNA repair
  • Exercise
  • Sirtuins
  • Skeletal muscle

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

Cite this

Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats. / Koltai, Erika; Szabo, Zsofia; Atalay, Mustafa; Boldogh, Istvan; Naito, Hisashi; Goto, Sataro; Nyakas, Csaba; Radak, Zsolt.

In: Mechanisms of Ageing and Development, Vol. 131, No. 1, 01.2010, p. 21-28.

Research output: Contribution to journalArticle

Koltai, Erika ; Szabo, Zsofia ; Atalay, Mustafa ; Boldogh, Istvan ; Naito, Hisashi ; Goto, Sataro ; Nyakas, Csaba ; Radak, Zsolt. / Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats. In: Mechanisms of Ageing and Development. 2010 ; Vol. 131, No. 1. pp. 21-28.
@article{f4046246b3d3464c9e7a964e534fd896,
title = "Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats",
abstract = "Silent information regulators are potent NAD+-dependent protein deacetylases, which have been shown to regulate gene silencing, muscle differentiation and DNA damage repair. Here, changes in the level and activity of sirtuin 1 (SIRT1) in response to exercise in groups of young and old rats were studied. There was an age-related increase in SIRT1 level, while exercise training significantly increased the relative activity of SIRT1. A strong inverse correlation was found between the nuclear activity of SIRT1 and the level of acetylated proteins. Exercise training induced SIRT1 activity due to the positive effect of exercise on the activity of nicotinamide phosphoribosyltransferase (NAMPT) and thereby the production of sirtuin-fueling NAD+. Exercise training normalized the age-associated shift in redox balance, since exercised animals had significantly lower levels of carbonylated proteins, expression of hypoxia-inducible factor-1alpha and vascular endothelial growth factor. The age-associated increase in the level of SIRT6 was attenuated by exercise training. On the other hand, aging did not significantly increase the level of DNA damage, which was in line with the activity of 8-oxoguanine DNA glycosylase, while exercise training increased the level of this enzyme. Regular exercise decelerates the deleterious effects of the aging process via SIRT1-dependent pathways through the stimulation of NAD+ biosynthesis by NAMPT.",
keywords = "Aging, DNA repair, Exercise, Sirtuins, Skeletal muscle",
author = "Erika Koltai and Zsofia Szabo and Mustafa Atalay and Istvan Boldogh and Hisashi Naito and Sataro Goto and Csaba Nyakas and Zsolt Radak",
year = "2010",
month = "1",
doi = "10.1016/j.mad.2009.11.002",
language = "English (US)",
volume = "131",
pages = "21--28",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats

AU - Koltai, Erika

AU - Szabo, Zsofia

AU - Atalay, Mustafa

AU - Boldogh, Istvan

AU - Naito, Hisashi

AU - Goto, Sataro

AU - Nyakas, Csaba

AU - Radak, Zsolt

PY - 2010/1

Y1 - 2010/1

N2 - Silent information regulators are potent NAD+-dependent protein deacetylases, which have been shown to regulate gene silencing, muscle differentiation and DNA damage repair. Here, changes in the level and activity of sirtuin 1 (SIRT1) in response to exercise in groups of young and old rats were studied. There was an age-related increase in SIRT1 level, while exercise training significantly increased the relative activity of SIRT1. A strong inverse correlation was found between the nuclear activity of SIRT1 and the level of acetylated proteins. Exercise training induced SIRT1 activity due to the positive effect of exercise on the activity of nicotinamide phosphoribosyltransferase (NAMPT) and thereby the production of sirtuin-fueling NAD+. Exercise training normalized the age-associated shift in redox balance, since exercised animals had significantly lower levels of carbonylated proteins, expression of hypoxia-inducible factor-1alpha and vascular endothelial growth factor. The age-associated increase in the level of SIRT6 was attenuated by exercise training. On the other hand, aging did not significantly increase the level of DNA damage, which was in line with the activity of 8-oxoguanine DNA glycosylase, while exercise training increased the level of this enzyme. Regular exercise decelerates the deleterious effects of the aging process via SIRT1-dependent pathways through the stimulation of NAD+ biosynthesis by NAMPT.

AB - Silent information regulators are potent NAD+-dependent protein deacetylases, which have been shown to regulate gene silencing, muscle differentiation and DNA damage repair. Here, changes in the level and activity of sirtuin 1 (SIRT1) in response to exercise in groups of young and old rats were studied. There was an age-related increase in SIRT1 level, while exercise training significantly increased the relative activity of SIRT1. A strong inverse correlation was found between the nuclear activity of SIRT1 and the level of acetylated proteins. Exercise training induced SIRT1 activity due to the positive effect of exercise on the activity of nicotinamide phosphoribosyltransferase (NAMPT) and thereby the production of sirtuin-fueling NAD+. Exercise training normalized the age-associated shift in redox balance, since exercised animals had significantly lower levels of carbonylated proteins, expression of hypoxia-inducible factor-1alpha and vascular endothelial growth factor. The age-associated increase in the level of SIRT6 was attenuated by exercise training. On the other hand, aging did not significantly increase the level of DNA damage, which was in line with the activity of 8-oxoguanine DNA glycosylase, while exercise training increased the level of this enzyme. Regular exercise decelerates the deleterious effects of the aging process via SIRT1-dependent pathways through the stimulation of NAD+ biosynthesis by NAMPT.

KW - Aging

KW - DNA repair

KW - Exercise

KW - Sirtuins

KW - Skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=74149085437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74149085437&partnerID=8YFLogxK

U2 - 10.1016/j.mad.2009.11.002

DO - 10.1016/j.mad.2009.11.002

M3 - Article

C2 - 19913571

AN - SCOPUS:74149085437

VL - 131

SP - 21

EP - 28

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 1

ER -