Exercise improves import of 8-oxoguanine DNA glycosylase into the mitochondrial matrix of skeletal muscle and enhances the relative activity

Zsolt Radak, Mustafa Atalay, Judit Jakus, Istvan Boldogh, Kelvin Davies, Sataro Goto

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Exercise has been shown to modify the level/activity of the DNA damage repair enzyme 8-oxoguanine-DNA glycosylase (OGG1) in skeletal muscle. We have studied the impact of regular physical training (8 weeks of swimming) and detraining (8 weeks of rest after an 8-week training session) on the activity of OGG1 in the nucleus and mitochondria as well as its targeting to the mitochondrial matrix in skeletal muscle. Neither exercise training nor detraining altered the overall levels of reactive species; however, mitochondrial levels of carbonylated proteins were decreased in the trained group as assessed by electron spin resonance and biochemical approaches. Importantly, nuclear OGG1 activity was increased by daily exercise training, whereas detraining reversed the up-regulating effect of training. Interestingly, training decreased the outer-membrane-associated mitochondrial OGG1 levels, whereas detraining reversed this effect. These results suggest that exercise training improves OGG1 import into the mitochondrial matrix, thereby increasing OGG1-mediated repair of oxidized guanine bases. Taken together, our data suggest that physical inactivity could impair the mitochondrial targeting of OGG1; however, exercise training increases OGG1 levels/activity in the nucleus and specific activity of OGG1 in mitochondrial compartments, thereby augmenting the repair of oxidized nuclear and mitochondrial DNA bases.

Original languageEnglish (US)
Pages (from-to)238-243
Number of pages6
JournalFree Radical Biology and Medicine
Volume46
Issue number2
DOIs
StatePublished - Jan 15 2009

Fingerprint

DNA Glycosylases
Muscle
Skeletal Muscle
Repair
Exercise
Mitochondria
Guanine
DNA Repair Enzymes
Mitochondrial DNA
Paramagnetic resonance
Electron Spin Resonance Spectroscopy
Mitochondrial Membranes
DNA Damage
Membranes
DNA
Enzymes
8-hydroxyguanine
Proteins

Keywords

  • Base excision repair
  • Detraining
  • Exercise
  • Free radicals
  • Nuclear mitochondrial OGG1

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Exercise improves import of 8-oxoguanine DNA glycosylase into the mitochondrial matrix of skeletal muscle and enhances the relative activity. / Radak, Zsolt; Atalay, Mustafa; Jakus, Judit; Boldogh, Istvan; Davies, Kelvin; Goto, Sataro.

In: Free Radical Biology and Medicine, Vol. 46, No. 2, 15.01.2009, p. 238-243.

Research output: Contribution to journalArticle

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