Exogenous amino acids stimulate human muscle anabolism without interfering with the response to mixed meal ingestion

Douglas Paddon-Jones, Melinda Sheffield-Moore, Asle Aarsland, Robert R. Wolfe, Arny A. Ferrando

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We sought to determine whether ingestion of a between-meal supplement containing 30 g of carbohydrate and 15 g of essential amino acids (CAA) altered the metabolic response to a nutritionally mixed meal in healthy, recreationally active male volunteers. A control group (CON; n = 6, 38 ± 8 yr, 86 ± 10 kg, 179 ± 3 cm) received a liquid mixed meal [protein, 23.4 ± 1.0 g (essential amino acids, 14.7 ± 0.7 g); carbohydrate, 126.6 ± 4.0 g; fat, 30.3 ± 2.8 g] every 5 h (0830, 1330, 1830). The experimental group (SUP; n = 7, 36 ± 10 yr, 87 ± 12 kg, 180 ± 3 cm) consumed the same meals but, in addition, were given CAA supplements (1100, 1600, 2100). Net phenylalanine balance (NB) and fractional synthetic rate (FSR) were calculated during a 16-h primed constant infusion of L-[ring-2H5]phenylalanine. Ingestion of a combination of CAA supplements and meals resulted in a greater mixed muscle FSR than ingestion of the meals alone (SUP, 0.099 ± 0.008; CON, 0.076 ± 0.005%/h; P < 0.05). Both groups experienced an improvement in NB after the morning (SUP, -2.2 ± 3.3; CON, -1.5 ± 3.5 nmol·min -1·100 ml leg volume-1) and evening meals (SUP, -9.7 ± 4.3; CON, -6.7 ± 4.1 nmol·min -1·100 ml leg volume-1). NB after CAA ingestion was significantly greater than after the meals, with values of 40.2 ± 8.5 nmol·min-1·100 ml leg volume-1. These data indicate that CAA supplementation produces a greater anabolic effect than ingestion of intact protein but does not interfere with the normal metabolic response to a meal.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume288
Issue number4 51-4
DOIs
StatePublished - Apr 2005

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Essential Amino Acids
Phenylalanine
Meals
Muscle
Eating
Carbohydrates
Anabolic Agents
Amino Acids
Muscles
Proteins
Fats
Leg
Liquids
Volunteers
Control Groups

Keywords

  • Diet
  • Protein metabolism
  • Skeletal muscle
  • Supplement

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Exogenous amino acids stimulate human muscle anabolism without interfering with the response to mixed meal ingestion. / Paddon-Jones, Douglas; Sheffield-Moore, Melinda; Aarsland, Asle; Wolfe, Robert R.; Ferrando, Arny A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 288, No. 4 51-4, 04.2005.

Research output: Contribution to journalArticle

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abstract = "We sought to determine whether ingestion of a between-meal supplement containing 30 g of carbohydrate and 15 g of essential amino acids (CAA) altered the metabolic response to a nutritionally mixed meal in healthy, recreationally active male volunteers. A control group (CON; n = 6, 38 ± 8 yr, 86 ± 10 kg, 179 ± 3 cm) received a liquid mixed meal [protein, 23.4 ± 1.0 g (essential amino acids, 14.7 ± 0.7 g); carbohydrate, 126.6 ± 4.0 g; fat, 30.3 ± 2.8 g] every 5 h (0830, 1330, 1830). The experimental group (SUP; n = 7, 36 ± 10 yr, 87 ± 12 kg, 180 ± 3 cm) consumed the same meals but, in addition, were given CAA supplements (1100, 1600, 2100). Net phenylalanine balance (NB) and fractional synthetic rate (FSR) were calculated during a 16-h primed constant infusion of L-[ring-2H5]phenylalanine. Ingestion of a combination of CAA supplements and meals resulted in a greater mixed muscle FSR than ingestion of the meals alone (SUP, 0.099 ± 0.008; CON, 0.076 ± 0.005{\%}/h; P < 0.05). Both groups experienced an improvement in NB after the morning (SUP, -2.2 ± 3.3; CON, -1.5 ± 3.5 nmol·min -1·100 ml leg volume-1) and evening meals (SUP, -9.7 ± 4.3; CON, -6.7 ± 4.1 nmol·min -1·100 ml leg volume-1). NB after CAA ingestion was significantly greater than after the meals, with values of 40.2 ± 8.5 nmol·min-1·100 ml leg volume-1. These data indicate that CAA supplementation produces a greater anabolic effect than ingestion of intact protein but does not interfere with the normal metabolic response to a meal.",
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