Exosomes mediate Zika virus transmission through SMPD3 neutral Sphingomyelinase in cortical neurons

Wenshuo Zhou, Michael Woodson, Michael Sherman, Girish Neelakanta, Hameeda Sultana

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The harmful effects of ZIKA virus (ZIKV) infection are reflected by severe neurological manifestations such as microcephaly in neonates and other complications associated with Guillain-Barré syndrome in adults. The transmission dynamics of ZIKV in or between neurons, or within the developing brains of the foetuses are not fully understood. Using primary cultures of murine cortical neurons, we show that ZIKV uses exosomes as mediators of viral transmission between neurons. Cryo-electron microscopy showed heterogeneous population of neuronal exosomes with a size range of 30-200 nm. Increased production of exosomes from neuronal cells was noted upon ZIKV infection. Neuronal exosomes contained both ZIKV viral RNA and protein(s) that were highly infectious to naïve cells. RNaseA and neutralizing antibodies treatment studies suggest the presence of viral RNA/proteins inside exosomes. Exosomes derived from time- and dose-dependent incubations showed increasing viral loads suggesting higher packaging and delivery of ZIKV RNA and proteins. Furthermore, we noted that ZIKV induced both activity and gene expression of neutral Sphingomyelinase (nSMase)-2/SMPD3, an important molecule that regulates production and release of exosomes. Silencing of SMPD3 in neurons resulted in reduced viral burden and transmission through exosomes. Treatment with SMPD3 specific inhibitor GW4869, significantly reduced ZIKV loads in both cortical neurons and in exosomes derived from these neuronal cells. Taken together, our results suggest that ZIKV modulates SMPD3 activity in cortical neurons for its infection and transmission through exosomes perhaps leading to severe neuronal death that may result in neurological manifestations such as microcephaly in the developing embryonic brains.

Original languageEnglish (US)
Pages (from-to)307-326
Number of pages20
JournalEmerging microbes & infections
Volume8
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Exosomes
Sphingomyelin Phosphodiesterase
Neurons
Viruses
Microcephaly
Viral RNA
Viral Proteins
Virus Diseases
Neurologic Manifestations
Viral Load
Zika Virus
Cryoelectron Microscopy
Infectious Disease Transmission
RNA Viruses
Brain
Product Packaging
Neutralizing Antibodies
Fetus

Keywords

  • cortical neurons
  • cryo-EM
  • Exosomes
  • GW4869
  • neutral Sphingomyelinase
  • transmission
  • ZIKA virus

ASJC Scopus subject areas

  • Epidemiology
  • Parasitology
  • Microbiology
  • Immunology
  • Drug Discovery
  • Infectious Diseases
  • Virology

Cite this

Exosomes mediate Zika virus transmission through SMPD3 neutral Sphingomyelinase in cortical neurons. / Zhou, Wenshuo; Woodson, Michael; Sherman, Michael; Neelakanta, Girish; Sultana, Hameeda.

In: Emerging microbes & infections, Vol. 8, No. 1, 01.01.2019, p. 307-326.

Research output: Contribution to journalArticle

Zhou, Wenshuo ; Woodson, Michael ; Sherman, Michael ; Neelakanta, Girish ; Sultana, Hameeda. / Exosomes mediate Zika virus transmission through SMPD3 neutral Sphingomyelinase in cortical neurons. In: Emerging microbes & infections. 2019 ; Vol. 8, No. 1. pp. 307-326.
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