Expansion of CUG RNA repeats causes stress and inhibition of translation in myotonic dystrophy 1 (DM1) cells

Claudia Huichalaf, Keiko Sakai, Bingwen Jin, Karlie Jones, Guo Li Wang, Benedikt Schoser, Christiane Schneider-Gold, Partha Sarkar, Olivia M. Pereira-Smith, Nikolai Timchenko, Lubov Timchenko

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

The purpose of this study was to investigate the role of the mutant CUGn RNA in the induction of stress in type 1 myotonic dystrophy (DM1) cells and in the stress-mediated inhibition of protein translation in DM1. To achieve our goals, we performed HPLC-based purification of stress granules (SGs), immunoanalysis of SGs with stress markers TIA-1, CUGBP1, and ph-eIF2, site-specific mutagenesis, and examinations of RNA-protein and proteinprotein interactions in myoblasts from control and DM1 patients. The cause-and-effect relationships were addressed in stable cells expressing mutant CUG repeats. We found that the mutant CUGn RNA induces formation of SGs through the increase of the double-stranded RNAdependent protein kinase (PKR) and following inactivation of eIF2α, one of the substrates of PKR. We show that SGs trap mRNA coding for the DNA repair and remodeling factor MRG15 (MORF4L1), translation of which is regulated by CUGBP1. As the result of the trapping, the levels of MRG15 are reduced in DM1 cells and in CUG-expressing cells. These data show thatCUGrepeats cause stress in DM1 through the PKR-ph-eIF2α pathway inhibiting translation of certain mRNAs, such as MRG15 mRNA. The repression of protein translation by stress might contribute to the progressive muscle loss in DM1.

Original languageEnglish (US)
Pages (from-to)3706-3719
Number of pages14
JournalFASEB Journal
Volume24
Issue number10
DOIs
StatePublished - Oct 2010

Keywords

  • CUG-binding protein
  • CUGBP1
  • MRG15/MORF
  • PKR

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Huichalaf, C., Sakai, K., Jin, B., Jones, K., Wang, G. L., Schoser, B., Schneider-Gold, C., Sarkar, P., Pereira-Smith, O. M., Timchenko, N., & Timchenko, L. (2010). Expansion of CUG RNA repeats causes stress and inhibition of translation in myotonic dystrophy 1 (DM1) cells. FASEB Journal, 24(10), 3706-3719. https://doi.org/10.1096/fj.09-151159