Experimental aerosol inoculation and investigation of potential lateral transmission of Mycobacterium bovis in virginia opossum (Didelphis virginiana)

Karla A. Fenton, Scott D. Fitzgerald, Steve Bolin, John Kaneene, James Sikarskie, Rena Greenwald, Konstantin Lyashchenko

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An endemic focus of Mycobacterium bovis (M. bovis) infection in the state of Michigan has contributed to a regional persistence in the animal population. The objective of this study was to determine if Virginia opossums (Didelphis virginiana) contribute to disease persistence by experimentally assessing intraspecies lateral transmission. One wild caught pregnant female opossum bearing 11 joeys (young opossum) and one age-matched joey were obtained for the study. Four joeys were aerosol inoculated with M. bovis (inoculated), four joeys were noninoculated (exposed), and four joeys plus the dam were controls. Four replicate groups of one inoculated and one exposed joey were housed together for 45 days commencing 7 days after experimental inoculation. At day 84 opossums were sacrificed. All four inoculated opossums had a positive test band via rapid test, culture positive, and gross/histologic lesions consistent with caseogranulomatous pneumonia. The exposed and control groups were unremarkable on gross, histology, rapid test, and culture. In conclusion, M. bovis infection within the inoculated opossums was confirmed by gross pathology, histopathology, bacterial culture, and antibody tests. However, M. bovis was not detected in the control and exposed opossums. There was no appreciable lateral transmission of M. bovis after aerosol inoculation and 45 days of cohabitation between infected and uninfected opossums.

Original languageEnglish (US)
Article number842861
JournalVeterinary Medicine International
DOIs
StatePublished - 2012
Externally publishedYes

Fingerprint

Didelphis
Didelphis virginiana
Opossums
Mycobacterium bovis
opossums
aerosols
Aerosols
Mycobacterium Infections
testing
Bacterial Antibodies
lesions (animal)
infection
histopathology
pneumonia
histology
Pneumonia
Histology
Pathology
Control Groups

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Experimental aerosol inoculation and investigation of potential lateral transmission of Mycobacterium bovis in virginia opossum (Didelphis virginiana). / Fenton, Karla A.; Fitzgerald, Scott D.; Bolin, Steve; Kaneene, John; Sikarskie, James; Greenwald, Rena; Lyashchenko, Konstantin.

In: Veterinary Medicine International, 2012.

Research output: Contribution to journalArticle

Fenton, Karla A. ; Fitzgerald, Scott D. ; Bolin, Steve ; Kaneene, John ; Sikarskie, James ; Greenwald, Rena ; Lyashchenko, Konstantin. / Experimental aerosol inoculation and investigation of potential lateral transmission of Mycobacterium bovis in virginia opossum (Didelphis virginiana). In: Veterinary Medicine International. 2012.
@article{e1f6a33e7dcb4f269ae19a5903ebb624,
title = "Experimental aerosol inoculation and investigation of potential lateral transmission of Mycobacterium bovis in virginia opossum (Didelphis virginiana)",
abstract = "An endemic focus of Mycobacterium bovis (M. bovis) infection in the state of Michigan has contributed to a regional persistence in the animal population. The objective of this study was to determine if Virginia opossums (Didelphis virginiana) contribute to disease persistence by experimentally assessing intraspecies lateral transmission. One wild caught pregnant female opossum bearing 11 joeys (young opossum) and one age-matched joey were obtained for the study. Four joeys were aerosol inoculated with M. bovis (inoculated), four joeys were noninoculated (exposed), and four joeys plus the dam were controls. Four replicate groups of one inoculated and one exposed joey were housed together for 45 days commencing 7 days after experimental inoculation. At day 84 opossums were sacrificed. All four inoculated opossums had a positive test band via rapid test, culture positive, and gross/histologic lesions consistent with caseogranulomatous pneumonia. The exposed and control groups were unremarkable on gross, histology, rapid test, and culture. In conclusion, M. bovis infection within the inoculated opossums was confirmed by gross pathology, histopathology, bacterial culture, and antibody tests. However, M. bovis was not detected in the control and exposed opossums. There was no appreciable lateral transmission of M. bovis after aerosol inoculation and 45 days of cohabitation between infected and uninfected opossums.",
author = "Fenton, {Karla A.} and Fitzgerald, {Scott D.} and Steve Bolin and John Kaneene and James Sikarskie and Rena Greenwald and Konstantin Lyashchenko",
year = "2012",
doi = "10.1155/2012/842861",
language = "English (US)",
journal = "Veterinary Medicine International",
issn = "2090-8113",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Experimental aerosol inoculation and investigation of potential lateral transmission of Mycobacterium bovis in virginia opossum (Didelphis virginiana)

AU - Fenton, Karla A.

AU - Fitzgerald, Scott D.

AU - Bolin, Steve

AU - Kaneene, John

AU - Sikarskie, James

AU - Greenwald, Rena

AU - Lyashchenko, Konstantin

PY - 2012

Y1 - 2012

N2 - An endemic focus of Mycobacterium bovis (M. bovis) infection in the state of Michigan has contributed to a regional persistence in the animal population. The objective of this study was to determine if Virginia opossums (Didelphis virginiana) contribute to disease persistence by experimentally assessing intraspecies lateral transmission. One wild caught pregnant female opossum bearing 11 joeys (young opossum) and one age-matched joey were obtained for the study. Four joeys were aerosol inoculated with M. bovis (inoculated), four joeys were noninoculated (exposed), and four joeys plus the dam were controls. Four replicate groups of one inoculated and one exposed joey were housed together for 45 days commencing 7 days after experimental inoculation. At day 84 opossums were sacrificed. All four inoculated opossums had a positive test band via rapid test, culture positive, and gross/histologic lesions consistent with caseogranulomatous pneumonia. The exposed and control groups were unremarkable on gross, histology, rapid test, and culture. In conclusion, M. bovis infection within the inoculated opossums was confirmed by gross pathology, histopathology, bacterial culture, and antibody tests. However, M. bovis was not detected in the control and exposed opossums. There was no appreciable lateral transmission of M. bovis after aerosol inoculation and 45 days of cohabitation between infected and uninfected opossums.

AB - An endemic focus of Mycobacterium bovis (M. bovis) infection in the state of Michigan has contributed to a regional persistence in the animal population. The objective of this study was to determine if Virginia opossums (Didelphis virginiana) contribute to disease persistence by experimentally assessing intraspecies lateral transmission. One wild caught pregnant female opossum bearing 11 joeys (young opossum) and one age-matched joey were obtained for the study. Four joeys were aerosol inoculated with M. bovis (inoculated), four joeys were noninoculated (exposed), and four joeys plus the dam were controls. Four replicate groups of one inoculated and one exposed joey were housed together for 45 days commencing 7 days after experimental inoculation. At day 84 opossums were sacrificed. All four inoculated opossums had a positive test band via rapid test, culture positive, and gross/histologic lesions consistent with caseogranulomatous pneumonia. The exposed and control groups were unremarkable on gross, histology, rapid test, and culture. In conclusion, M. bovis infection within the inoculated opossums was confirmed by gross pathology, histopathology, bacterial culture, and antibody tests. However, M. bovis was not detected in the control and exposed opossums. There was no appreciable lateral transmission of M. bovis after aerosol inoculation and 45 days of cohabitation between infected and uninfected opossums.

UR - http://www.scopus.com/inward/record.url?scp=84863659853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863659853&partnerID=8YFLogxK

U2 - 10.1155/2012/842861

DO - 10.1155/2012/842861

M3 - Article

JO - Veterinary Medicine International

JF - Veterinary Medicine International

SN - 2090-8113

M1 - 842861

ER -