Experimental approaches for investigating ion atmospheres around nucleic acids and proteins

Binhan Yu; Junji Iwahara

doi:10.1016/j.csbj.2021.04.033

Experimental approaches for investigating ion atmospheres around nucleic acids and proteins

Binhan Yu, Junji Iwahara

Biochemistry & Molecular Biology

Research output: Contribution to journal › Review article › peer-review

10 Scopus citations

Abstract

Ionic interactions are crucial to biological functions of DNA, RNA, and proteins. Experimental research on how ions behave around biological macromolecules has lagged behind corresponding theoretical and computational research. In the 21st century, quantitative experimental approaches for investigating ionic interactions of biomolecules have become available and greatly facilitated examinations of theoretical electrostatic models. These approaches utilize anomalous small-angle X-ray scattering, atomic emission spectroscopy, mass spectrometry, or nuclear magnetic resonance (NMR) spectroscopy. We provide an overview on the experimental methodologies that can quantify and characterize ions within the ion atmospheres around nucleic acids, proteins, and their complexes.

Original language	English (US)
Pages (from-to)	2279-2285
Number of pages	7
Journal	Computational and Structural Biotechnology Journal
Volume	19
DOIs	https://doi.org/10.1016/j.csbj.2021.04.033
State	Published - Jan 2021

Keywords

Dynamics
Electrostatic interactions
Ion atmosphere
Ionic diffusion
Spatial distribution

ASJC Scopus subject areas

Biotechnology
Biophysics
Structural Biology
Biochemistry
Genetics
Computer Science Applications

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10.1016/j.csbj.2021.04.033

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title = "Experimental approaches for investigating ion atmospheres around nucleic acids and proteins",

abstract = "Ionic interactions are crucial to biological functions of DNA, RNA, and proteins. Experimental research on how ions behave around biological macromolecules has lagged behind corresponding theoretical and computational research. In the 21st century, quantitative experimental approaches for investigating ionic interactions of biomolecules have become available and greatly facilitated examinations of theoretical electrostatic models. These approaches utilize anomalous small-angle X-ray scattering, atomic emission spectroscopy, mass spectrometry, or nuclear magnetic resonance (NMR) spectroscopy. We provide an overview on the experimental methodologies that can quantify and characterize ions within the ion atmospheres around nucleic acids, proteins, and their complexes.",

keywords = "Dynamics, Electrostatic interactions, Ion atmosphere, Ionic diffusion, Spatial distribution",

author = "Binhan Yu and Junji Iwahara",

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T1 - Experimental approaches for investigating ion atmospheres around nucleic acids and proteins

AU - Yu, Binhan

AU - Iwahara, Junji

N1 - Funding Information: This work was supported by Grant R35-GM130326 (to J.I.) from the National Institutes of Health. We thank Channing Pletka for editing the manuscript. Publisher Copyright: © 2021 The Author(s)

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Y1 - 2021/1

N2 - Ionic interactions are crucial to biological functions of DNA, RNA, and proteins. Experimental research on how ions behave around biological macromolecules has lagged behind corresponding theoretical and computational research. In the 21st century, quantitative experimental approaches for investigating ionic interactions of biomolecules have become available and greatly facilitated examinations of theoretical electrostatic models. These approaches utilize anomalous small-angle X-ray scattering, atomic emission spectroscopy, mass spectrometry, or nuclear magnetic resonance (NMR) spectroscopy. We provide an overview on the experimental methodologies that can quantify and characterize ions within the ion atmospheres around nucleic acids, proteins, and their complexes.

AB - Ionic interactions are crucial to biological functions of DNA, RNA, and proteins. Experimental research on how ions behave around biological macromolecules has lagged behind corresponding theoretical and computational research. In the 21st century, quantitative experimental approaches for investigating ionic interactions of biomolecules have become available and greatly facilitated examinations of theoretical electrostatic models. These approaches utilize anomalous small-angle X-ray scattering, atomic emission spectroscopy, mass spectrometry, or nuclear magnetic resonance (NMR) spectroscopy. We provide an overview on the experimental methodologies that can quantify and characterize ions within the ion atmospheres around nucleic acids, proteins, and their complexes.

KW - Dynamics

KW - Electrostatic interactions

KW - Ion atmosphere

KW - Ionic diffusion

KW - Spatial distribution

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DO - 10.1016/j.csbj.2021.04.033

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AN - SCOPUS:85105694864

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VL - 19

SP - 2279

EP - 2285

JO - Computational and Structural Biotechnology Journal

JF - Computational and Structural Biotechnology Journal

ER -

Experimental approaches for investigating ion atmospheres around nucleic acids and proteins

Abstract

Keywords

ASJC Scopus subject areas

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