Experimental detection of Rift Valley fever virus by reverse transcription-polymerase chain reaction assay in large samples of mosquitoes

P. G. Jupp, A. A. Grobbelaar, P. A. Leman, A. Kemp, R. F. Dunton, T. R. Burkot, Thomas Ksiazek, R. Swanepoel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) was assessed in laboratory tests to detect the presence of single Aedes aegypti (L.) or Eretmapodites quinquevittatus Theobald mosquitoes infected with Rift Valley fever virus in pools of mosquitoes, 50-600 in size, from laboratory colonies or mixed field collections. The viral RNA was detected in all pools containing infected mosquitoes and was shown to be as sensitive as infant mice but more sensitive than Vero cell cultures for virus detection. Pools diluted down to the equivalent of 1:16 000 mosquitoes were also positive by RT-PCR. RNAs from 4 other phleboviruses were negative, there were no false positives and the procedure followed, with the 2 particular primers chosen, gave consistently clear bands of the PCR products on agarose gels without nested PCR being necessary.

Original languageEnglish (US)
Pages (from-to)467-471
Number of pages5
JournalJournal of Medical Entomology
Volume37
Issue number3
StatePublished - May 2000
Externally publishedYes

Fingerprint

Rift Valley fever virus
Culicidae
Reverse Transcription
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction
assays
Phlebovirus
RNA
sampling
Vero Cells
Aedes
Viral RNA
Aedes aegypti
Sepharose
agarose
cell culture
Cell Culture Techniques
Gels
gels
Viruses

Keywords

  • Mosquitoes
  • Reverse transcription-polymerase chain reaction
  • Rift valley fever virus

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Jupp, P. G., Grobbelaar, A. A., Leman, P. A., Kemp, A., Dunton, R. F., Burkot, T. R., ... Swanepoel, R. (2000). Experimental detection of Rift Valley fever virus by reverse transcription-polymerase chain reaction assay in large samples of mosquitoes. Journal of Medical Entomology, 37(3), 467-471.

Experimental detection of Rift Valley fever virus by reverse transcription-polymerase chain reaction assay in large samples of mosquitoes. / Jupp, P. G.; Grobbelaar, A. A.; Leman, P. A.; Kemp, A.; Dunton, R. F.; Burkot, T. R.; Ksiazek, Thomas; Swanepoel, R.

In: Journal of Medical Entomology, Vol. 37, No. 3, 05.2000, p. 467-471.

Research output: Contribution to journalArticle

Jupp, PG, Grobbelaar, AA, Leman, PA, Kemp, A, Dunton, RF, Burkot, TR, Ksiazek, T & Swanepoel, R 2000, 'Experimental detection of Rift Valley fever virus by reverse transcription-polymerase chain reaction assay in large samples of mosquitoes', Journal of Medical Entomology, vol. 37, no. 3, pp. 467-471.
Jupp, P. G. ; Grobbelaar, A. A. ; Leman, P. A. ; Kemp, A. ; Dunton, R. F. ; Burkot, T. R. ; Ksiazek, Thomas ; Swanepoel, R. / Experimental detection of Rift Valley fever virus by reverse transcription-polymerase chain reaction assay in large samples of mosquitoes. In: Journal of Medical Entomology. 2000 ; Vol. 37, No. 3. pp. 467-471.
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