Experimental Ehrlichia chaffeensis infection in beagles

Xiao Feng Zhang, Jianzhi Zhang, S. Wesley Long, Randall P. Ruble, Xue Jie Yu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A canine model for human monocytic ehrlichiosis was used to assess persistent infection and antigenic variation of Ehrlichia chaffeensis. Two beagle dogs were infected subcutaneously with E chaffeensis Arkansas strain. The dogs were observed for 6 months after inoculation for clinical signs, blood chemistry changes, antibodies to E. chaffeensis and presence of E. chaffeensis in the blood. Both dogs developed thrombocytopenia, but exhibited normal body temperatures during the entire course of infection. In one dog, E. chaffeensis was cultivated for up to 74 days post-inoculation and E. chaffeensis DNA was detected in the dog's blood for up to 81 days. In the other dog, E. chaffeensis was cultured for up to 102 days and E. chaffeensis DNA was detected in the blood for up to 117 days. PCR amplification and DNA sequence analysis indicated that there was no genetic variation in the 120 kDa outer-membrane glycoprotein gene of E. chaffeensis during infection of the dogs. The dogs developed antibodies to the immunodominant proteins of E. chaffeensis, including the 175, 140, 120, 80, 50 and 28 kDa proteins, starting in the fifth week post-inoculation. The dogs maintained high antibody titres throughout the 6-month study period. These results indicate that dogs become carriers of E. chaffeensis for 2-4 months after infection without exhibiting signs of clinical disease, suggesting that dogs may serve as a natural host for E. chaffeensis.

Original languageEnglish (US)
Pages (from-to)1021-1026
Number of pages6
JournalJournal of Medical Microbiology
Volume52
Issue number11
DOIs
StatePublished - Nov 2003

Fingerprint

Ehrlichia chaffeensis
Dogs
Infection
Antibodies
Ehrlichiosis
Dog Diseases
Antigenic Variation
DNA
Membrane Glycoproteins
Body Temperature
DNA Sequence Analysis
Thrombocytopenia

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Zhang, X. F., Zhang, J., Long, S. W., Ruble, R. P., & Yu, X. J. (2003). Experimental Ehrlichia chaffeensis infection in beagles. Journal of Medical Microbiology, 52(11), 1021-1026. https://doi.org/10.1099/jmm.0.05234-0

Experimental Ehrlichia chaffeensis infection in beagles. / Zhang, Xiao Feng; Zhang, Jianzhi; Long, S. Wesley; Ruble, Randall P.; Yu, Xue Jie.

In: Journal of Medical Microbiology, Vol. 52, No. 11, 11.2003, p. 1021-1026.

Research output: Contribution to journalArticle

Zhang, XF, Zhang, J, Long, SW, Ruble, RP & Yu, XJ 2003, 'Experimental Ehrlichia chaffeensis infection in beagles', Journal of Medical Microbiology, vol. 52, no. 11, pp. 1021-1026. https://doi.org/10.1099/jmm.0.05234-0
Zhang, Xiao Feng ; Zhang, Jianzhi ; Long, S. Wesley ; Ruble, Randall P. ; Yu, Xue Jie. / Experimental Ehrlichia chaffeensis infection in beagles. In: Journal of Medical Microbiology. 2003 ; Vol. 52, No. 11. pp. 1021-1026.
@article{a875805799ea48f5ac7e77135ae18727,
title = "Experimental Ehrlichia chaffeensis infection in beagles",
abstract = "A canine model for human monocytic ehrlichiosis was used to assess persistent infection and antigenic variation of Ehrlichia chaffeensis. Two beagle dogs were infected subcutaneously with E chaffeensis Arkansas strain. The dogs were observed for 6 months after inoculation for clinical signs, blood chemistry changes, antibodies to E. chaffeensis and presence of E. chaffeensis in the blood. Both dogs developed thrombocytopenia, but exhibited normal body temperatures during the entire course of infection. In one dog, E. chaffeensis was cultivated for up to 74 days post-inoculation and E. chaffeensis DNA was detected in the dog's blood for up to 81 days. In the other dog, E. chaffeensis was cultured for up to 102 days and E. chaffeensis DNA was detected in the blood for up to 117 days. PCR amplification and DNA sequence analysis indicated that there was no genetic variation in the 120 kDa outer-membrane glycoprotein gene of E. chaffeensis during infection of the dogs. The dogs developed antibodies to the immunodominant proteins of E. chaffeensis, including the 175, 140, 120, 80, 50 and 28 kDa proteins, starting in the fifth week post-inoculation. The dogs maintained high antibody titres throughout the 6-month study period. These results indicate that dogs become carriers of E. chaffeensis for 2-4 months after infection without exhibiting signs of clinical disease, suggesting that dogs may serve as a natural host for E. chaffeensis.",
author = "Zhang, {Xiao Feng} and Jianzhi Zhang and Long, {S. Wesley} and Ruble, {Randall P.} and Yu, {Xue Jie}",
year = "2003",
month = "11",
doi = "10.1099/jmm.0.05234-0",
language = "English (US)",
volume = "52",
pages = "1021--1026",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "11",

}

TY - JOUR

T1 - Experimental Ehrlichia chaffeensis infection in beagles

AU - Zhang, Xiao Feng

AU - Zhang, Jianzhi

AU - Long, S. Wesley

AU - Ruble, Randall P.

AU - Yu, Xue Jie

PY - 2003/11

Y1 - 2003/11

N2 - A canine model for human monocytic ehrlichiosis was used to assess persistent infection and antigenic variation of Ehrlichia chaffeensis. Two beagle dogs were infected subcutaneously with E chaffeensis Arkansas strain. The dogs were observed for 6 months after inoculation for clinical signs, blood chemistry changes, antibodies to E. chaffeensis and presence of E. chaffeensis in the blood. Both dogs developed thrombocytopenia, but exhibited normal body temperatures during the entire course of infection. In one dog, E. chaffeensis was cultivated for up to 74 days post-inoculation and E. chaffeensis DNA was detected in the dog's blood for up to 81 days. In the other dog, E. chaffeensis was cultured for up to 102 days and E. chaffeensis DNA was detected in the blood for up to 117 days. PCR amplification and DNA sequence analysis indicated that there was no genetic variation in the 120 kDa outer-membrane glycoprotein gene of E. chaffeensis during infection of the dogs. The dogs developed antibodies to the immunodominant proteins of E. chaffeensis, including the 175, 140, 120, 80, 50 and 28 kDa proteins, starting in the fifth week post-inoculation. The dogs maintained high antibody titres throughout the 6-month study period. These results indicate that dogs become carriers of E. chaffeensis for 2-4 months after infection without exhibiting signs of clinical disease, suggesting that dogs may serve as a natural host for E. chaffeensis.

AB - A canine model for human monocytic ehrlichiosis was used to assess persistent infection and antigenic variation of Ehrlichia chaffeensis. Two beagle dogs were infected subcutaneously with E chaffeensis Arkansas strain. The dogs were observed for 6 months after inoculation for clinical signs, blood chemistry changes, antibodies to E. chaffeensis and presence of E. chaffeensis in the blood. Both dogs developed thrombocytopenia, but exhibited normal body temperatures during the entire course of infection. In one dog, E. chaffeensis was cultivated for up to 74 days post-inoculation and E. chaffeensis DNA was detected in the dog's blood for up to 81 days. In the other dog, E. chaffeensis was cultured for up to 102 days and E. chaffeensis DNA was detected in the blood for up to 117 days. PCR amplification and DNA sequence analysis indicated that there was no genetic variation in the 120 kDa outer-membrane glycoprotein gene of E. chaffeensis during infection of the dogs. The dogs developed antibodies to the immunodominant proteins of E. chaffeensis, including the 175, 140, 120, 80, 50 and 28 kDa proteins, starting in the fifth week post-inoculation. The dogs maintained high antibody titres throughout the 6-month study period. These results indicate that dogs become carriers of E. chaffeensis for 2-4 months after infection without exhibiting signs of clinical disease, suggesting that dogs may serve as a natural host for E. chaffeensis.

UR - http://www.scopus.com/inward/record.url?scp=1542541073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542541073&partnerID=8YFLogxK

U2 - 10.1099/jmm.0.05234-0

DO - 10.1099/jmm.0.05234-0

M3 - Article

C2 - 14532348

AN - SCOPUS:1542541073

VL - 52

SP - 1021

EP - 1026

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 11

ER -