Experimental hepatitis E

Pathogenesis in cynomolgus macaques (Macaca fascicularis)

Charles F. Longer, Stephen L. Denny, Jeffrey D. Caudill, Thomas A. Miele, Ludmila V S Asher, K. S A Myint, Chiao Chain Huang, Walter F. Engler, James LeDuc, Leonard N. Binn, John R. Ticehurst

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The pathogenesis of experimental hepatitis E has not been thoroughly investigated. The purpose of this study was to more accurately document the events in this disease. Cynomolgus macaques were inoculated intravenously with bile or feces containing hepatitis E virus (HEV). Serum, bile, and liver specimens were evaluated with light microscopy, immune electron microscopy, immunofluorescence microscopy, EIA, and polymerase chain reaction. In the third week, there were histopathologic changes and HEV antigen (HEVAg) in liver, HEV in bile, and alanine aminotransferase (ALT) elevations. Widespread pathologic changes were detected during the fourth week and antibody to HEV (anti-HEV) and peak ALT values in the fifth or sixth week. By the sixth week, HEVAg had disappeared but pathologic changes persisted. This study supports the concept that experimental hepatitis E has an initial phase in which hepatic HEV replication is accompanied by the onset of hepatitis and a later phase in which the appearance of anti-HEV is accompanied by progression of the hepatitis.

Original languageEnglish (US)
Pages (from-to)602-609
Number of pages8
JournalJournal of Infectious Diseases
Volume168
Issue number3
StatePublished - Sep 1993
Externally publishedYes

Fingerprint

Hepatitis E
Hepatitis E virus
Macaca fascicularis
Macaca
Bile
Alanine Transaminase
Hepatitis
Liver
Antigens
Antibodies
Virus Replication
Fluorescence Microscopy
Feces
Microscopy
Electron Microscopy
Light
Polymerase Chain Reaction
Serum

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Longer, C. F., Denny, S. L., Caudill, J. D., Miele, T. A., Asher, L. V. S., Myint, K. S. A., ... Ticehurst, J. R. (1993). Experimental hepatitis E: Pathogenesis in cynomolgus macaques (Macaca fascicularis). Journal of Infectious Diseases, 168(3), 602-609.

Experimental hepatitis E : Pathogenesis in cynomolgus macaques (Macaca fascicularis). / Longer, Charles F.; Denny, Stephen L.; Caudill, Jeffrey D.; Miele, Thomas A.; Asher, Ludmila V S; Myint, K. S A; Huang, Chiao Chain; Engler, Walter F.; LeDuc, James; Binn, Leonard N.; Ticehurst, John R.

In: Journal of Infectious Diseases, Vol. 168, No. 3, 09.1993, p. 602-609.

Research output: Contribution to journalArticle

Longer, CF, Denny, SL, Caudill, JD, Miele, TA, Asher, LVS, Myint, KSA, Huang, CC, Engler, WF, LeDuc, J, Binn, LN & Ticehurst, JR 1993, 'Experimental hepatitis E: Pathogenesis in cynomolgus macaques (Macaca fascicularis)', Journal of Infectious Diseases, vol. 168, no. 3, pp. 602-609.
Longer CF, Denny SL, Caudill JD, Miele TA, Asher LVS, Myint KSA et al. Experimental hepatitis E: Pathogenesis in cynomolgus macaques (Macaca fascicularis). Journal of Infectious Diseases. 1993 Sep;168(3):602-609.
Longer, Charles F. ; Denny, Stephen L. ; Caudill, Jeffrey D. ; Miele, Thomas A. ; Asher, Ludmila V S ; Myint, K. S A ; Huang, Chiao Chain ; Engler, Walter F. ; LeDuc, James ; Binn, Leonard N. ; Ticehurst, John R. / Experimental hepatitis E : Pathogenesis in cynomolgus macaques (Macaca fascicularis). In: Journal of Infectious Diseases. 1993 ; Vol. 168, No. 3. pp. 602-609.
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