Experimental Hepatitis E: Pathogenesis in Cynomolgus Macaques (Macaca fascicularis)

Charles F. Longer, Stephen L. Denny, Jeffrey D. Caudill, Thomas A. Miele, Ludmila V.S. Asher, K. S.A. Myint, Chiao Chain Huang, Walter F. Engler, James W. LeDuc, Leonard N. Binn, John R. Ticehurst, Charles F. Longer, Stephen L. Denny, Jeffrey D. Caudill, Thomas A. Miele, Ludmila V.S. Asher, K. S.A. Myint, Chiao Chain Huang, Walter F. Engler, James W. LeDucLeonard N. Binn, John R. Ticehurst, Charles F. Longer, Stephen L. Denny, Jeffrey D. Caudill, Thomas A. Miele, Ludmila V.S. Asher, K. S.A. Myint, Chiao Chain Huang, Walter F. Engler, James W. LeDuc, Leonard N. Binn, John R. Ticehurst, Charles F. Longer, Stephen L. Denny, Jeffrey D. Caudill, Thomas A. Miele, Ludmila V.S. Asher, K. S.A. Myint, Chiao Chain Huang, Walter F. Engler, James W. LeDuc, Leonard N. Binn, John R. Ticehurst, Stephen L. Denny, James W. LeDuc

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

The pathogenesis of experimental hepatitis E has not been thoroughly investigated. The purpose of this study was to more accurately document the events in this disease. Cynomolgus macaques were inoculated intravenously with bile or feces containing hepatitis E virus (HEV). Serum, bile, and liver specimens were evaluated with light microscopy, immune electron microscopy, immunofluorescence microscopy, EIA, and polymerase chain reaction. In the third week, there were histopathologic changes and HEV antigen (HEVAg) in liver, HEV in bile, and alanine aminotransferase (ALT) elevations. Widespread pathologic changes were detected during the fourth week and antibody to HEV (anti-HEV) and peak ALT values in the fifth or sixth week. By the sixth week, HEVAg had disappeared but pathologic changes persisted. This study supports the concept that experimental hepatitis E has an initial phase in which hepatic HEV replication is accompanied by the onset of hepatitis and a later phase in which the appearance ofanti-HEV is accompanied by progression of the hepatitis.

Original languageEnglish (US)
Pages (from-to)602-609
Number of pages8
JournalJournal of Infectious Diseases
Volume168
Issue number3
DOIs
StatePublished - Sep 1993
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Longer, C. F., Denny, S. L., Caudill, J. D., Miele, T. A., Asher, L. V. S., Myint, K. S. A., Huang, C. C., Engler, W. F., LeDuc, J. W., Binn, L. N., Ticehurst, J. R., Longer, C. F., Denny, S. L., Caudill, J. D., Miele, T. A., Asher, L. V. S., Myint, K. S. A., Huang, C. C., Engler, W. F., ... LeDuc, J. W. (1993). Experimental Hepatitis E: Pathogenesis in Cynomolgus Macaques (Macaca fascicularis). Journal of Infectious Diseases, 168(3), 602-609. https://doi.org/10.1093/infdis/168.3.602