Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989-1990 U.S. epizootic

P. B. Jahrling, Thomas Geisbert, N. K. Jaax, M. A. Hanes, Thomas Ksiazek, C. J. Peters

Research output: Contribution to journalArticle

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Abstract

This study describes the pathogenesis of the Ebola-Reston (EBO-R) subtype of Ebola virus for experimentally infected cynomolgus monkeys. The disease course of EBO-R in macaques was very similar to human disease and to experimental diseases in macaques following EBO-Zaire and EBO-Sudan infections. Cynomolgus monkeys infected with EBO-R in this experiment developed anorexia, occasional nasal discharge, and splenomegaly, petechial facial hemorrhages and severe subcutaneous hemorrhages in venipuncture sites, similar to human Ebola fever. Five of the six EBO-R infected monkeys died, 8 to 14 days after inoculation. One survived and developed high titered neutralizing antibodies specific for EBO-R. The five acutely ill monkeys shed infectious virus in various bodily secretions. Further, abundant virus was visualized in alveolar interstitial cells and free in the alveoli suggesting the potential for generating infectious aerosols. Thus, taking precautions against aerosol exposures to filovirus infected primates, including humans, seems prudent. This experiment demonstrated that EBO-R was lethal for macaques and was capable of initiating and sustaining the monkey epizootic. Further investigation of this animal model should facilitate development of effective immunization, treatment, and control strategies for Ebola hemorrhagic fever.

Original languageEnglish (US)
Pages (from-to)115-134
Number of pages20
JournalArchives of Virology, Supplement
Volume1996
Issue number11
StatePublished - 1996
Externally publishedYes

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Macaca
Haplorhini
Macaca fascicularis
Infection
Aerosols
Bodily Secretions
Ebola Hemorrhagic Fever
Ebolavirus
Hemorrhage
Viruses
Alveolar Epithelial Cells
Democratic Republic of the Congo
Sudan
Phlebotomy
Splenomegaly
Anorexia
Neutralizing Antibodies
Nose
Primates
Immunization

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989-1990 U.S. epizootic. / Jahrling, P. B.; Geisbert, Thomas; Jaax, N. K.; Hanes, M. A.; Ksiazek, Thomas; Peters, C. J.

In: Archives of Virology, Supplement, Vol. 1996, No. 11, 1996, p. 115-134.

Research output: Contribution to journalArticle

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