Experimental infection of guinea pigs with Venezuelan hemorrhagic fever virus (Guanarito)

A model of human disease

William C. Hall, Thomas Geisbert, John W. Huggins, Peter B. Jahrling

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Venezuelan hemorrhagic fever (VHF), a newly described disease caused by an arenavirus (Guanarito), has resulted in multiple human deaths in Venezuela. To develop an animal model of this disease, strain 13 and Hartley strain guinea pigs were inoculated subcutaneously with Guananto strain 95551 of arenavirus in a pilot study to determine susceptibility of the species to the virus. All animals were killed when moribund 12-14 days following inoculation. Animals were necropsied and tissues were fixed and examined by both light and electron microscopy. Viral antigen was demonstrated in the tissues by immunohistochemistry at both the light and electron microscopic levels. Lesions were characterized by single cell necrosis of epithelium of the gastrointestinal tract, interstitial pneumonia, lymphoid and hematopoietic cell necrosis, and the presence of platelet thrombi in occasional blood vessels associated with hemorrhage. Viral antigen was demonstrated in lymphoid tissues and macrophages, endothelial cells of multiple organs, pulmonary epithelium, epithelium of the gastrointestinal tract, and in miscellaneous other tissues and cells. Intact virions and typical arenavirus inclusions were demonstrated by immunoelectron microscopy in these tissues. Based on these findings, the guinea pig appears to be a valid animal model of the human disease.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume55
Issue number1
StatePublished - Jul 1996
Externally publishedYes

Fingerprint

New World Arenaviruses
Arenavirus
Guinea Pigs
Fever
Animal Disease Models
Epithelium
Viral Antigens
Infection
Gastrointestinal Tract
Necrosis
Light
Venezuela
Immunoelectron Microscopy
Interstitial Lung Diseases
Lymphoid Tissue
Virion
Blood Vessels
Electron Microscopy
Thrombosis
Blood Platelets

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Experimental infection of guinea pigs with Venezuelan hemorrhagic fever virus (Guanarito) : A model of human disease. / Hall, William C.; Geisbert, Thomas; Huggins, John W.; Jahrling, Peter B.

In: American Journal of Tropical Medicine and Hygiene, Vol. 55, No. 1, 07.1996, p. 81-88.

Research output: Contribution to journalArticle

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