Experimental Infection of Rhesus Macaques with West Nile Virus

Level and Duration of Viremia and Kinetics of the Antibody Response after Infection

Marion S. Ratterree, Robin A. Gutierrez, Amelia P A Travassos Da Rosa, Bruce J. Dille, David W C Beasley, Rudolf P. Bohm, Suresh M. Desai, Peter J. Didier, Larry G. Bikenmeyer, George J. Dawson, Thomas P. Leary, Gerald Schochetman, Katherine Phillippi-Falkenstein, Juan Arroyo, Alan D T Barrett, Robert B. Tesh

Research output: Contribution to journalArticle

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Abstract

Reports of transfusion-associated cases of West Nile virus (WNV) infection indicate the need for sensitive screening methods to identify WNV-infected blood products. We experimentally infected 5 rhesus macaques with WNV, to determine the level and duration of viremia, the kinetics of the humoral immune response, and the sensitivity of various assay systems for detecting WNV in blood. All macaques developed subclinical infections with low levels of viremia; nested reverse-transcription polymerase chain reaction was the most sensitive method for detecting virus or viral RNA in blood. Specific WNV antibodies appeared during the second week of infection; the results of an IgM enzyme-linked immunosorbent assay became positive on the ninth or tenth day after infection, followed in 1-2 days by hemagglutination-inhibiting and neutralizing antibodies. Our results suggest that both nucleic acid and serological testing may be needed to determine exposure to WNV and to identify potentially infected blood donors.

Original languageEnglish (US)
Pages (from-to)669-676
Number of pages8
JournalJournal of Infectious Diseases
Volume189
Issue number4
DOIs
StatePublished - Feb 15 2004

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West Nile virus
Viremia
Macaca mulatta
Antibody Formation
Infection
Asymptomatic Infections
Viral RNA
Hemagglutination
Macaca
Virus Diseases
Humoral Immunity
Neutralizing Antibodies
Blood Donors
Nucleic Acids
Reverse Transcription
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay
Viruses
Polymerase Chain Reaction
Antibodies

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Experimental Infection of Rhesus Macaques with West Nile Virus : Level and Duration of Viremia and Kinetics of the Antibody Response after Infection. / Ratterree, Marion S.; Gutierrez, Robin A.; Travassos Da Rosa, Amelia P A; Dille, Bruce J.; Beasley, David W C; Bohm, Rudolf P.; Desai, Suresh M.; Didier, Peter J.; Bikenmeyer, Larry G.; Dawson, George J.; Leary, Thomas P.; Schochetman, Gerald; Phillippi-Falkenstein, Katherine; Arroyo, Juan; Barrett, Alan D T; Tesh, Robert B.

In: Journal of Infectious Diseases, Vol. 189, No. 4, 15.02.2004, p. 669-676.

Research output: Contribution to journalArticle

Ratterree, MS, Gutierrez, RA, Travassos Da Rosa, APA, Dille, BJ, Beasley, DWC, Bohm, RP, Desai, SM, Didier, PJ, Bikenmeyer, LG, Dawson, GJ, Leary, TP, Schochetman, G, Phillippi-Falkenstein, K, Arroyo, J, Barrett, ADT & Tesh, RB 2004, 'Experimental Infection of Rhesus Macaques with West Nile Virus: Level and Duration of Viremia and Kinetics of the Antibody Response after Infection', Journal of Infectious Diseases, vol. 189, no. 4, pp. 669-676. https://doi.org/10.1086/381461
Ratterree, Marion S. ; Gutierrez, Robin A. ; Travassos Da Rosa, Amelia P A ; Dille, Bruce J. ; Beasley, David W C ; Bohm, Rudolf P. ; Desai, Suresh M. ; Didier, Peter J. ; Bikenmeyer, Larry G. ; Dawson, George J. ; Leary, Thomas P. ; Schochetman, Gerald ; Phillippi-Falkenstein, Katherine ; Arroyo, Juan ; Barrett, Alan D T ; Tesh, Robert B. / Experimental Infection of Rhesus Macaques with West Nile Virus : Level and Duration of Viremia and Kinetics of the Antibody Response after Infection. In: Journal of Infectious Diseases. 2004 ; Vol. 189, No. 4. pp. 669-676.
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