Experimental Infection of Syrian Hamsters with Aerosolized Nipah Virus

Olivier Escaffre, Terence Hill, Tetsuro Ikegami, Terry L. Juelich, Jennifer K. Smith, Lihong Zhang, David E. Perez, Colm Atkins, Arnold Park, William Lawrence, Satheesh K. Sivasubramani, Jennifer E. Peel, Johnny Peterson, Benhur Lee, Alexander Freiberg

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background. Nipah virus (NiV) is a paramyxovirus (genus Henipavirus) that can cause severe respiratory illness and encephalitis in humans. Transmission occurs through consumption of NiV-contaminated foods, and contact with NiV-infected animals or human body fluids. However, it is unclear whether aerosols derived from aforesaid sources or others also contribute to transmission, and current knowledge on NiV-induced pathogenicity after small-particle aerosol exposure is still limited. Methods. Infectivity, pathogenicity, and real-time dissemination of aerosolized NiV in Syrian hamsters was evaluated using NiV-Malaysia (NiV-M) and/or its recombinant expressing firefly luciferase (rNiV-FlucNP). Results. Both viruses had an equivalent pathogenicity in hamsters, which developed respiratory and neurological symptoms of disease, similar to using intranasal route, with no direct correlations to the dose. We showed that virus replication was predominantly initiated in the lower respiratory tract and, although delayed, also intensely in the oronasal cavity and possibly the brain, with gradual increase of signal in these regions until at least day 5-6 postinfection. Conclusion. Hamsters infected with small-particle aerosolized NiV undergo similar clinical manifestations of the disease as previously described using liquid inoculum, and exhibit histopathological lesions consistent with NiV patient reports. NiV droplets could therefore play a role in transmission by close contact.

Original languageEnglish (US)
Pages (from-to)1602-1610
Number of pages9
JournalJournal of Infectious Diseases
Volume218
Issue number10
DOIs
StatePublished - Oct 5 2018

Fingerprint

Nipah Virus
Mesocricetus
Infection
Virulence
Aerosols
Cricetinae
Henipavirus
Firefly Luciferases
Malaysia
Body Fluids
Encephalitis
Virus Replication
Human Body
Respiratory System

Keywords

  • aerosol
  • animal model
  • hamster
  • henipavirus
  • in vivo imaging.
  • Nipah virus
  • pathogenesis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Experimental Infection of Syrian Hamsters with Aerosolized Nipah Virus. / Escaffre, Olivier; Hill, Terence; Ikegami, Tetsuro; Juelich, Terry L.; Smith, Jennifer K.; Zhang, Lihong; Perez, David E.; Atkins, Colm; Park, Arnold; Lawrence, William; Sivasubramani, Satheesh K.; Peel, Jennifer E.; Peterson, Johnny; Lee, Benhur; Freiberg, Alexander.

In: Journal of Infectious Diseases, Vol. 218, No. 10, 05.10.2018, p. 1602-1610.

Research output: Contribution to journalArticle

Escaffre, O, Hill, T, Ikegami, T, Juelich, TL, Smith, JK, Zhang, L, Perez, DE, Atkins, C, Park, A, Lawrence, W, Sivasubramani, SK, Peel, JE, Peterson, J, Lee, B & Freiberg, A 2018, 'Experimental Infection of Syrian Hamsters with Aerosolized Nipah Virus', Journal of Infectious Diseases, vol. 218, no. 10, pp. 1602-1610. https://doi.org/10.1093/infdis/jiy357
Escaffre, Olivier ; Hill, Terence ; Ikegami, Tetsuro ; Juelich, Terry L. ; Smith, Jennifer K. ; Zhang, Lihong ; Perez, David E. ; Atkins, Colm ; Park, Arnold ; Lawrence, William ; Sivasubramani, Satheesh K. ; Peel, Jennifer E. ; Peterson, Johnny ; Lee, Benhur ; Freiberg, Alexander. / Experimental Infection of Syrian Hamsters with Aerosolized Nipah Virus. In: Journal of Infectious Diseases. 2018 ; Vol. 218, No. 10. pp. 1602-1610.
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AU - Smith, Jennifer K.

AU - Zhang, Lihong

AU - Perez, David E.

AU - Atkins, Colm

AU - Park, Arnold

AU - Lawrence, William

AU - Sivasubramani, Satheesh K.

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AU - Peterson, Johnny

AU - Lee, Benhur

AU - Freiberg, Alexander

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