Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus). II. Pathology

S. Y. Xiao, H. Zhang, H. Guzman, R. B. Tesh

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Subadult and adult hamsters were inoculated intraperitoneally with 106 TCID50 of yellow fever (YF) virus (Jimenez strain). Four animals from each group were subjected daily to histologic examination for 9 days. The liver showed spotty necrosis on day 3 after infection, which was followed by steatosis and focally confluent necrosis. In surviving hamsters, hepatocyte regeneration began on day 8, which was accompanied by decreasing steatosis. The spleen initially exhibited lymphoid hyperplasia, which was followed by lymphoid depletion and increased phagocytosis by splenic macrophages. Focal pancreatic acinar necrosis and spotty adrenal cortical necrosis were seen transiently between days 5 and 7. Viral antigen was detected immunohistochemically in the liver and the spleen. TUNEL analysis showed a dynamic change of hepatocyte necrapoptosis, with activity corresponding to the severity of disease. The histopathologic changes were more severe in younger (subadult) animals. The YF-hamster model appears to be an accurate and inexpensive experimental system for studying the pathophysiology and treatment of YF.

Original languageEnglish (US)
Pages (from-to)1437-1444
Number of pages8
JournalJournal of Infectious Diseases
Volume183
Issue number10
DOIs
StatePublished - May 15 2001

Fingerprint

Yellow fever virus
Mesocricetus
Virus Diseases
Necrosis
Cricetinae
Pathology
Yellow Fever
Hepatocytes
Spleen
Viral Antigens
Liver
In Situ Nick-End Labeling
Phagocytosis
Hyperplasia
Regeneration
Macrophages
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus). II. Pathology. / Xiao, S. Y.; Zhang, H.; Guzman, H.; Tesh, R. B.

In: Journal of Infectious Diseases, Vol. 183, No. 10, 15.05.2001, p. 1437-1444.

Research output: Contribution to journalArticle

Xiao, S. Y. ; Zhang, H. ; Guzman, H. ; Tesh, R. B. / Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus). II. Pathology. In: Journal of Infectious Diseases. 2001 ; Vol. 183, No. 10. pp. 1437-1444.
@article{487a4245da4449869f90de5897e73446,
title = "Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus). II. Pathology",
abstract = "Subadult and adult hamsters were inoculated intraperitoneally with 106 TCID50 of yellow fever (YF) virus (Jimenez strain). Four animals from each group were subjected daily to histologic examination for 9 days. The liver showed spotty necrosis on day 3 after infection, which was followed by steatosis and focally confluent necrosis. In surviving hamsters, hepatocyte regeneration began on day 8, which was accompanied by decreasing steatosis. The spleen initially exhibited lymphoid hyperplasia, which was followed by lymphoid depletion and increased phagocytosis by splenic macrophages. Focal pancreatic acinar necrosis and spotty adrenal cortical necrosis were seen transiently between days 5 and 7. Viral antigen was detected immunohistochemically in the liver and the spleen. TUNEL analysis showed a dynamic change of hepatocyte necrapoptosis, with activity corresponding to the severity of disease. The histopathologic changes were more severe in younger (subadult) animals. The YF-hamster model appears to be an accurate and inexpensive experimental system for studying the pathophysiology and treatment of YF.",
author = "Xiao, {S. Y.} and H. Zhang and H. Guzman and Tesh, {R. B.}",
year = "2001",
month = "5",
day = "15",
doi = "10.1086/320200",
language = "English (US)",
volume = "183",
pages = "1437--1444",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus). II. Pathology

AU - Xiao, S. Y.

AU - Zhang, H.

AU - Guzman, H.

AU - Tesh, R. B.

PY - 2001/5/15

Y1 - 2001/5/15

N2 - Subadult and adult hamsters were inoculated intraperitoneally with 106 TCID50 of yellow fever (YF) virus (Jimenez strain). Four animals from each group were subjected daily to histologic examination for 9 days. The liver showed spotty necrosis on day 3 after infection, which was followed by steatosis and focally confluent necrosis. In surviving hamsters, hepatocyte regeneration began on day 8, which was accompanied by decreasing steatosis. The spleen initially exhibited lymphoid hyperplasia, which was followed by lymphoid depletion and increased phagocytosis by splenic macrophages. Focal pancreatic acinar necrosis and spotty adrenal cortical necrosis were seen transiently between days 5 and 7. Viral antigen was detected immunohistochemically in the liver and the spleen. TUNEL analysis showed a dynamic change of hepatocyte necrapoptosis, with activity corresponding to the severity of disease. The histopathologic changes were more severe in younger (subadult) animals. The YF-hamster model appears to be an accurate and inexpensive experimental system for studying the pathophysiology and treatment of YF.

AB - Subadult and adult hamsters were inoculated intraperitoneally with 106 TCID50 of yellow fever (YF) virus (Jimenez strain). Four animals from each group were subjected daily to histologic examination for 9 days. The liver showed spotty necrosis on day 3 after infection, which was followed by steatosis and focally confluent necrosis. In surviving hamsters, hepatocyte regeneration began on day 8, which was accompanied by decreasing steatosis. The spleen initially exhibited lymphoid hyperplasia, which was followed by lymphoid depletion and increased phagocytosis by splenic macrophages. Focal pancreatic acinar necrosis and spotty adrenal cortical necrosis were seen transiently between days 5 and 7. Viral antigen was detected immunohistochemically in the liver and the spleen. TUNEL analysis showed a dynamic change of hepatocyte necrapoptosis, with activity corresponding to the severity of disease. The histopathologic changes were more severe in younger (subadult) animals. The YF-hamster model appears to be an accurate and inexpensive experimental system for studying the pathophysiology and treatment of YF.

UR - http://www.scopus.com/inward/record.url?scp=0035873046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035873046&partnerID=8YFLogxK

U2 - 10.1086/320200

DO - 10.1086/320200

M3 - Article

C2 - 11319680

AN - SCOPUS:0035873046

VL - 183

SP - 1437

EP - 1444

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 10

ER -