Explorative study on isolation and characterization of a microviridae G4 bacteriophage, EMCL318, against multi-drug-resistant Escherichia coli 15-318

Soumya Ghosh, Emma Persad, Ting Yun Shiue, Cindy Lam, Afsana Islam, Lauren G. Mascibroda, Michael Sherman, Thomas Smith, Naowarat Cheeptham

Research output: Contribution to journalArticle

Abstract

Bacteriophages screened and isolated from sewage water samples exhibited antibacterial activities against multi-drug-resistant Escherichia coli strains. Five different water samples from Canadian habitats such as Kamloops Wastewater Treatment Center, Domtar, the Pacific Ocean, Bisaro Anima Cave, and alkali ponds, were used in this study. Four Enterobacteriaceae strains including one non-resistant and three clinical multi-drug Escherichia coli strains (E. coli 15-102, E. coli 15-124, and E. coli 15-318) were selected as target bacteria to screen for the bacteriophages from these collected water samples. Seeded agar assay technique was implemented for the screening. It was found that only sewage water sample exhibited a significant number of plaques count with the E. coli 15-318 (1.82 × 102 plaques/plate) cells in comparison to E. coli non-resistant strain K12 (8 plaques/plate). The phage did not produce plaques in the E. coli 15-124 and E. coli 15-102 strains. The bacteriophage, designated EMCL318, was isolated, purified, characterized, and identified to belong to the G4 species of the Family Microviridae, GenBank accession number MG563770. This is an explorative study conducted in order to reveal the viruses as alternative potentials to fight against emerging and existing multi-drug-resistant infectious diseases.

Original languageEnglish (US)
Article number92
JournalAntibiotics
Volume7
Issue number4
DOIs
StatePublished - Dec 1 2018

Fingerprint

Microviridae
Microvirus
Bacteriophages
Escherichia coli
Pharmaceutical Preparations
Water
Sewage
Pacific Ocean
Caves
Nucleic Acid Databases
Alkalies
Ponds
Enterobacteriaceae
Waste Water
Viruses
Wastewater treatment
Agar
Communicable Diseases
Ecosystem

Keywords

  • Alternative therapeutic measures
  • Bacteriophages
  • Multi-drug-resistant organisms
  • Phage therapy

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Explorative study on isolation and characterization of a microviridae G4 bacteriophage, EMCL318, against multi-drug-resistant Escherichia coli 15-318. / Ghosh, Soumya; Persad, Emma; Shiue, Ting Yun; Lam, Cindy; Islam, Afsana; Mascibroda, Lauren G.; Sherman, Michael; Smith, Thomas; Cheeptham, Naowarat.

In: Antibiotics, Vol. 7, No. 4, 92, 01.12.2018.

Research output: Contribution to journalArticle

Ghosh, Soumya ; Persad, Emma ; Shiue, Ting Yun ; Lam, Cindy ; Islam, Afsana ; Mascibroda, Lauren G. ; Sherman, Michael ; Smith, Thomas ; Cheeptham, Naowarat. / Explorative study on isolation and characterization of a microviridae G4 bacteriophage, EMCL318, against multi-drug-resistant Escherichia coli 15-318. In: Antibiotics. 2018 ; Vol. 7, No. 4.
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