TY - JOUR
T1 - Explorative study on isolation and characterization of a microviridae G4 bacteriophage, EMCL318, against multi-drug-resistant Escherichia coli 15-318
AU - Ghosh, Soumya
AU - Persad, Emma
AU - Shiue, Ting Yun
AU - Lam, Cindy
AU - Islam, Afsana
AU - Mascibroda, Lauren G.
AU - Sherman, Michael B.
AU - Smith, Thomas
AU - Cheeptham, Naowarat
N1 - Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2018/12
Y1 - 2018/12
N2 - Bacteriophages screened and isolated from sewage water samples exhibited antibacterial activities against multi-drug-resistant Escherichia coli strains. Five different water samples from Canadian habitats such as Kamloops Wastewater Treatment Center, Domtar, the Pacific Ocean, Bisaro Anima Cave, and alkali ponds, were used in this study. Four Enterobacteriaceae strains including one non-resistant and three clinical multi-drug Escherichia coli strains (E. coli 15-102, E. coli 15-124, and E. coli 15-318) were selected as target bacteria to screen for the bacteriophages from these collected water samples. Seeded agar assay technique was implemented for the screening. It was found that only sewage water sample exhibited a significant number of plaques count with the E. coli 15-318 (1.82 × 102 plaques/plate) cells in comparison to E. coli non-resistant strain K12 (8 plaques/plate). The phage did not produce plaques in the E. coli 15-124 and E. coli 15-102 strains. The bacteriophage, designated EMCL318, was isolated, purified, characterized, and identified to belong to the G4 species of the Family Microviridae, GenBank accession number MG563770. This is an explorative study conducted in order to reveal the viruses as alternative potentials to fight against emerging and existing multi-drug-resistant infectious diseases.
AB - Bacteriophages screened and isolated from sewage water samples exhibited antibacterial activities against multi-drug-resistant Escherichia coli strains. Five different water samples from Canadian habitats such as Kamloops Wastewater Treatment Center, Domtar, the Pacific Ocean, Bisaro Anima Cave, and alkali ponds, were used in this study. Four Enterobacteriaceae strains including one non-resistant and three clinical multi-drug Escherichia coli strains (E. coli 15-102, E. coli 15-124, and E. coli 15-318) were selected as target bacteria to screen for the bacteriophages from these collected water samples. Seeded agar assay technique was implemented for the screening. It was found that only sewage water sample exhibited a significant number of plaques count with the E. coli 15-318 (1.82 × 102 plaques/plate) cells in comparison to E. coli non-resistant strain K12 (8 plaques/plate). The phage did not produce plaques in the E. coli 15-124 and E. coli 15-102 strains. The bacteriophage, designated EMCL318, was isolated, purified, characterized, and identified to belong to the G4 species of the Family Microviridae, GenBank accession number MG563770. This is an explorative study conducted in order to reveal the viruses as alternative potentials to fight against emerging and existing multi-drug-resistant infectious diseases.
KW - Alternative therapeutic measures
KW - Bacteriophages
KW - Multi-drug-resistant organisms
KW - Phage therapy
UR - http://www.scopus.com/inward/record.url?scp=85056638039&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056638039&partnerID=8YFLogxK
U2 - 10.3390/antibiotics7040092
DO - 10.3390/antibiotics7040092
M3 - Article
C2 - 30373114
AN - SCOPUS:85056638039
SN - 2079-6382
VL - 7
JO - Antibiotics
JF - Antibiotics
IS - 4
M1 - 92
ER -