Expression and genetic variation of the Aplysia egg-laying hormone gene family in the atrial gland

A. Kurosky, E. L. Gorham, W. R A Van Heumen, A. T. Garcia, J. S. Smith, R. C. Mifflin

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    We have screened an Aplysia atrial gland cDNA library using an egg-laying hormone (ELH) precursor probe and have isolated and characterized five different clones, four of which are full-length and approximately 0.8 kb in size. The characterization of these cDNA clones firmly established the genetic variation of the ELH-related precursors expressed in the atrial gland and provided a rational basis for their revised nomenclature proposed herein. The five precursor ELH-related cDNA sequences obtained predicted the following genetically distinct polypeptide precursors designated as: A, [Asp143]A, [Glu94,Gln139]A, [Pro25]B, and [Phe96,Asp107]BT. The [Phe96,Asp107]BT cDNA sequence predicted a truncated form of a B-type precursor. Northern blot analysis of atrial gland RNA identified two transcripts of about equal intensity of 0.9 kb and 1.1 kb. Polymerase chain reaction of genomic DNA, together with DNA sequence analysis, resolved previously reported discrepancies between genomic and cDNA sequences of the ELH-related precursors. Taken together the results obtained identified the expression of five ELH-related precursor genes in the atrial gland of Aplysia from at lease two genetic loci per haploid genome.

    Original languageEnglish (US)
    Pages (from-to)261-271
    Number of pages11
    JournalInvertebrate Neuroscience
    Volume2
    Issue number4
    DOIs
    StatePublished - 1997

    Fingerprint

    Ovum
    Hormones
    Complementary DNA
    Aplysia
    Genes
    Clone Cells
    Genetic Loci
    Haploidy
    Gene Library
    DNA Sequence Analysis
    Terminology
    Northern Blotting
    Mollusca egg-laying hormone
    Genome
    RNA
    Polymerase Chain Reaction
    Peptides
    DNA

    Keywords

    • Aplysia californica
    • Atrial gland
    • Egg-laying hormone
    • Genetic variation

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Cellular and Molecular Neuroscience
    • Developmental Neuroscience

    Cite this

    Kurosky, A., Gorham, E. L., Van Heumen, W. R. A., Garcia, A. T., Smith, J. S., & Mifflin, R. C. (1997). Expression and genetic variation of the Aplysia egg-laying hormone gene family in the atrial gland. Invertebrate Neuroscience, 2(4), 261-271. https://doi.org/10.1007/BF02211939

    Expression and genetic variation of the Aplysia egg-laying hormone gene family in the atrial gland. / Kurosky, A.; Gorham, E. L.; Van Heumen, W. R A; Garcia, A. T.; Smith, J. S.; Mifflin, R. C.

    In: Invertebrate Neuroscience, Vol. 2, No. 4, 1997, p. 261-271.

    Research output: Contribution to journalArticle

    Kurosky, A, Gorham, EL, Van Heumen, WRA, Garcia, AT, Smith, JS & Mifflin, RC 1997, 'Expression and genetic variation of the Aplysia egg-laying hormone gene family in the atrial gland', Invertebrate Neuroscience, vol. 2, no. 4, pp. 261-271. https://doi.org/10.1007/BF02211939
    Kurosky, A. ; Gorham, E. L. ; Van Heumen, W. R A ; Garcia, A. T. ; Smith, J. S. ; Mifflin, R. C. / Expression and genetic variation of the Aplysia egg-laying hormone gene family in the atrial gland. In: Invertebrate Neuroscience. 1997 ; Vol. 2, No. 4. pp. 261-271.
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