Expression and localisation of FoxO3 and FoxO4 in human placenta and fetal membranes

M. Lappas, R. Lim, C. Riley, Ramkumar Menon, M. Permezel

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Forkhead box O (FoxO) proteins regulate inflammation, extracellular matrix (ECM) remodelling and apoptosis. We have previously identified FoxO1 proteins in human gestational tissues, and demonstrated a link between FoxO1 and rupture of fetal membranes. There is, however, no data available on the expression and localisation of FoxO3 and FoxO4 in human intrauterine tissues. Thus the aim of this study was to characterise the localisation and expression of FoxO3 and FoxO4 in (i) human placenta and fetal membranes before term spontaneous labour onset, and (ii) supracervical site (SCS) and distal site (DS) fetal membranes from non-labouring women. Immunohistochemistry, Western blotting and quantitative RT-PCR (qRT-PCR) was used to localise and quantitate FoxO3 and FoxO4 protein and mRNA expressions. Cytoplasmic and nuclear FoxO3 was localised in the syncytiotrophoblast layer, chorionic trophoblasts, amnion epithelium and decidua. Cytoplasmic FoxO4 was localised in the syncytiotrophoblasts and chorionic trophoblasts. No or very little FoxO4 protein and mRNA was present in amnion epithelium. The intensity and extent of staining of FoxO3 and FoxO4 was greater in fetal membranes obtained from the SCS compared to DS. Presence of FoxO3 and FoxO4 are expected to contribute to apoptosis and/or cell cycle regulation associated with fetal membrane rupture.

Original languageEnglish (US)
Pages (from-to)1043-1050
Number of pages8
JournalPlacenta
Volume31
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Extraembryonic Membranes
Placenta
Trophoblasts
Amnion
Rupture
Epithelium
Apoptosis
Forkhead Transcription Factors
Labor Onset
Decidua
Messenger RNA
Proteins
Extracellular Matrix
Cell Cycle
Western Blotting
Immunohistochemistry
Staining and Labeling
Inflammation
Polymerase Chain Reaction

Keywords

  • Fetal membrane rupture
  • Fetal membranes
  • FoxO3
  • FoxO4
  • Placenta

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Expression and localisation of FoxO3 and FoxO4 in human placenta and fetal membranes. / Lappas, M.; Lim, R.; Riley, C.; Menon, Ramkumar; Permezel, M.

In: Placenta, Vol. 31, No. 12, 12.2010, p. 1043-1050.

Research output: Contribution to journalArticle

Lappas, M. ; Lim, R. ; Riley, C. ; Menon, Ramkumar ; Permezel, M. / Expression and localisation of FoxO3 and FoxO4 in human placenta and fetal membranes. In: Placenta. 2010 ; Vol. 31, No. 12. pp. 1043-1050.
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