Expression and properties of bacteriophage T4 gene product 11

L. P. Kurochkina, Petr Leiman, S. Yu Venyaminov, V. V. Mesyanzhinov

Research output: Contribution to journalArticle

Abstract

A plasmid vector for expression of bacteriophage T4 gene product 11 (gp11) in E. coli cells has been constructed. Gp11 is a baseplate protein that connects short tail fibers providing irreversible adsorption of the virus on a cell. A method based on chromatography on hydroxyapatite has been developed for purification of recombinant gp11. The protein is active in an in vitro complementation assay and transforms defective phage particles lacking gp11 into infective ones. Gel filtration data suggest that the biologically active protein is a trimer. According to CD spectroscopy and sequence analysis data, the polypeptide chain of gp11 contains not less than 20% alpha-helical segments, about 30% beta-structure, and belongs to the class of alpha/beta structural proteins.

Original languageEnglish (US)
Pages (from-to)180-186
Number of pages7
JournalBiokhimiya
Volume66
Issue number2
StatePublished - 2001
Externally publishedYes

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Keywords

  • Bacteriophage T4
  • Base plate
  • Gene product 11
  • Protein folding

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Kurochkina, L. P., Leiman, P., Venyaminov, S. Y., & Mesyanzhinov, V. V. (2001). Expression and properties of bacteriophage T4 gene product 11. Biokhimiya, 66(2), 180-186.