Expression and properties of bacteriophage T4 gene product 11

L. P. Kurochkina, Petr Leiman, S. Yu Venyaminov, V. V. Mesyanzhinov

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A plasmid vector for expression of bacteriophage T4 gene product 11 (gp11) in E. coli cells has been constructed. Gp11 is a baseplate protein that connects short tail fibers providing irreversible adsorption of the virus on a cell. A method based on chromatography on hydroxyapatite has been developed for purification of recombinant gp11. The protein is active in an in vitro complementation assay and transforms defective phage particles lacking gp11 into infective ones. Gel filtration data suggest that the biologically active protein is a trimer. According to CD spectroscopy and sequence analysis data, the polypeptide chain of gp11 contains not less than 20% α-helical segments, about 30% β-structure, and belongs to the class of α/β structural proteins.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalBiochemistry (Moscow)
Volume66
Issue number2
DOIs
StatePublished - Feb 2001
Externally publishedYes

Fingerprint

Bacteriophage T4
Bacteriophages
Genes
Proteins
Durapatite
Chromatography
Viruses
Escherichia coli
Adsorption
Purification
Gel Chromatography
Sequence Analysis
Assays
Spectrum Analysis
Plasmids
Gels
Spectroscopy
Peptides
Fibers

Keywords

  • Bacteriophage T4
  • Baseplate
  • Gene product 11
  • Protein folding

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Expression and properties of bacteriophage T4 gene product 11. / Kurochkina, L. P.; Leiman, Petr; Venyaminov, S. Yu; Mesyanzhinov, V. V.

In: Biochemistry (Moscow), Vol. 66, No. 2, 02.2001, p. 141-146.

Research output: Contribution to journalArticle

Kurochkina, LP, Leiman, P, Venyaminov, SY & Mesyanzhinov, VV 2001, 'Expression and properties of bacteriophage T4 gene product 11', Biochemistry (Moscow), vol. 66, no. 2, pp. 141-146. https://doi.org/10.1023/A:1002831212462
Kurochkina, L. P. ; Leiman, Petr ; Venyaminov, S. Yu ; Mesyanzhinov, V. V. / Expression and properties of bacteriophage T4 gene product 11. In: Biochemistry (Moscow). 2001 ; Vol. 66, No. 2. pp. 141-146.
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