Expression and properties of bacteriophage T4 gene product 11

L. P. Kurochkina, P. G. Leiman, S. Yu Venyaminov, V. V. Mesyanzhinov

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A plasmid vector for expression of bacteriophage T4 gene product 11 (gp11) in E. coli cells has been constructed. Gp11 is a baseplate protein that connects short tail fibers providing irreversible adsorption of the virus on a cell. A method based on chromatography on hydroxyapatite has been developed for purification of recombinant gp11. The protein is active in an in vitro complementation assay and transforms defective phage particles lacking gp11 into infective ones. Gel filtration data suggest that the biologically active protein is a trimer. According to CD spectroscopy and sequence analysis data, the polypeptide chain of gp11 contains not less than 20% α-helical segments, about 30% β-structure, and belongs to the class of α/β structural proteins.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalBiochemistry (Moscow)
Volume66
Issue number2
DOIs
StatePublished - Feb 1 2001
Externally publishedYes

Keywords

  • Bacteriophage T4
  • Baseplate
  • Gene product 11
  • Protein folding

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Expression and properties of bacteriophage T4 gene product 11'. Together they form a unique fingerprint.

Cite this