Expression of a novel gene, MafB, in Dupuytren's disease

Lucy C. Lee, Andrew Y. Zhang, Alphonsus K. Chong, Hung Pham, Michael T. Longaker, James Chang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Purpose: Dupuytren's disease (DD) is characterized by fibroblastic proliferation of the palmar fascia, often leading to flexion contracture in the hand. Although there is a strong genetic component the genome-wide expression of novel genes is not known. The purpose of this study was to use DNA microarray technology to identify upregulated genes in DD. Methods: Human tissue samples were harvested from 3 patient sources: DD cord tissue (n = 20), normal-appearing adjacent control fascia (n = 15), and palmar fascia from patients having carpal tunnel release (n = 15). DNA microarray analysis was performed on amplified sample RNA. Novel genes were compared with known gene functions. A candidate gene of interest was studied further by using immunohistochemistry on DD tissue samples and controls. Results: Several novel genes not described previously in the study of DD were upregulated significantly, including MafB, collagen type V, α-2 (COL5A2), collagen type VIII, α-1 (COL8A1), contactin I (CNTN1), and leucine-rich repeat containing 17 (LRRC17). These upregulated genes were compared with their known gene-expression profiles in other tissues and their purported functions. MafB was found to be of particular interest because of its prominent role in tissue development and cellular differentiation. MafB immunohistochemistry showed positive staining in 50% of the DD specimens but complete absence of MafB in all control tissues (adjacent control fascia, carpal tunnel fascia). Co-localization experiments with MafB and α-smooth muscle actin showed staining properties in similar regions but these 2 proteins were not confined solely to the same cells. Conclusions: Microarray analysis of DD tissue has identified significant upregulated gene expression of MafB. MafB protein also is found in Dupuytren's cords but not in control fascia. Co-localization data suggest that the association of MafB with DD is not related exclusively to myofibroblast proliferation. Because of its role in fibroblastic transformation in other models MafB and its relationship to the pathogenesis of DD deserves further study.

Original languageEnglish (US)
Pages (from-to)211-218
Number of pages8
JournalJournal of Hand Surgery
Volume31
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Dupuytren Contracture
Fascia
Genes
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Wrist
MafB Transcription Factor
Contactins
Genome Components
Immunohistochemistry
Staining and Labeling
Gene Expression
Myofibroblasts
Collagen Type II
Contracture
Collagen Type I
Transcriptome
Leucine
Smooth Muscle
Actins

Keywords

  • Dupuytren's
  • MafB
  • Myofibroblast

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Lee, L. C., Zhang, A. Y., Chong, A. K., Pham, H., Longaker, M. T., & Chang, J. (2006). Expression of a novel gene, MafB, in Dupuytren's disease. Journal of Hand Surgery, 31(2), 211-218. https://doi.org/10.1016/j.jhsa.2005.09.007

Expression of a novel gene, MafB, in Dupuytren's disease. / Lee, Lucy C.; Zhang, Andrew Y.; Chong, Alphonsus K.; Pham, Hung; Longaker, Michael T.; Chang, James.

In: Journal of Hand Surgery, Vol. 31, No. 2, 02.2006, p. 211-218.

Research output: Contribution to journalArticle

Lee, LC, Zhang, AY, Chong, AK, Pham, H, Longaker, MT & Chang, J 2006, 'Expression of a novel gene, MafB, in Dupuytren's disease', Journal of Hand Surgery, vol. 31, no. 2, pp. 211-218. https://doi.org/10.1016/j.jhsa.2005.09.007
Lee LC, Zhang AY, Chong AK, Pham H, Longaker MT, Chang J. Expression of a novel gene, MafB, in Dupuytren's disease. Journal of Hand Surgery. 2006 Feb;31(2):211-218. https://doi.org/10.1016/j.jhsa.2005.09.007
Lee, Lucy C. ; Zhang, Andrew Y. ; Chong, Alphonsus K. ; Pham, Hung ; Longaker, Michael T. ; Chang, James. / Expression of a novel gene, MafB, in Dupuytren's disease. In: Journal of Hand Surgery. 2006 ; Vol. 31, No. 2. pp. 211-218.
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abstract = "Purpose: Dupuytren's disease (DD) is characterized by fibroblastic proliferation of the palmar fascia, often leading to flexion contracture in the hand. Although there is a strong genetic component the genome-wide expression of novel genes is not known. The purpose of this study was to use DNA microarray technology to identify upregulated genes in DD. Methods: Human tissue samples were harvested from 3 patient sources: DD cord tissue (n = 20), normal-appearing adjacent control fascia (n = 15), and palmar fascia from patients having carpal tunnel release (n = 15). DNA microarray analysis was performed on amplified sample RNA. Novel genes were compared with known gene functions. A candidate gene of interest was studied further by using immunohistochemistry on DD tissue samples and controls. Results: Several novel genes not described previously in the study of DD were upregulated significantly, including MafB, collagen type V, α-2 (COL5A2), collagen type VIII, α-1 (COL8A1), contactin I (CNTN1), and leucine-rich repeat containing 17 (LRRC17). These upregulated genes were compared with their known gene-expression profiles in other tissues and their purported functions. MafB was found to be of particular interest because of its prominent role in tissue development and cellular differentiation. MafB immunohistochemistry showed positive staining in 50{\%} of the DD specimens but complete absence of MafB in all control tissues (adjacent control fascia, carpal tunnel fascia). Co-localization experiments with MafB and α-smooth muscle actin showed staining properties in similar regions but these 2 proteins were not confined solely to the same cells. Conclusions: Microarray analysis of DD tissue has identified significant upregulated gene expression of MafB. MafB protein also is found in Dupuytren's cords but not in control fascia. Co-localization data suggest that the association of MafB with DD is not related exclusively to myofibroblast proliferation. Because of its role in fibroblastic transformation in other models MafB and its relationship to the pathogenesis of DD deserves further study.",
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