Expression of an RNAi-resistant SLBP restores proper S-phase progression

E. J. Wagner, A. Berkow, W. F. Marzluff

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Metazoan histone mRNAs are unique in that they lack a 3′- polyadenylated tail, but instead end in a conserved stem loop that is bound by SLBP (stem-loop binding protein). SLBP is required for efficient histone mRNA synthesis and translation. Removal of SLBP by RNA interference causes an increase in the number of cells in S-phase and this effect can be reversed by expressing an exogenous SLBP resistant to the small interfering RNA. Cells with decreased SLBP levels progress slowly through S-phase when released from a double-thymidine block. Thus SLBP is required for efficient DNA replication probably because a decreased ability to assemble chromatin results in a decrease in the rate of DNA replication.

Original languageEnglish (US)
Pages (from-to)471-473
Number of pages3
JournalBiochemical Society Transactions
Volume33
Issue number3
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

Keywords

  • Cell cycle
  • Histone mRNA
  • RNA interference
  • S-phase progression
  • Smell interfering RNA
  • Stem-loop binding protein

ASJC Scopus subject areas

  • Biochemistry

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