Expression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient

Pithi Chanvorachote, Sudjit Luanpitpong, Preedakorn Chunhacha, Worrawat Promden, Virote Sriuranpong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Advances in understanding lung cancer biology and tumor markers aid clinicians in managing the disease. Cancer-associated antigen (CA)125 has garnered increasing attention in lung cancer research and may benefit the treatment and follow-up of this type of cancer. In Thai lung cancer patients, knowledge regarding ethnic differences in cancer cell biology is largely absent. We generated lung cancer cells from the pleural effusion fluids of a Thai patient and designated these as P1 cells. P1 cells were assessed for growth rate, response to chemotherapy, and the presence of tumor markers, in particular CA125 expression. Results of immunofluorescence indicated that P1 cells exhibited strong expression levels of CA125, comparable to that of established H460 lung cancer cells. Furthermore, P1 cells were analyzed for the expression of additional markers. Results revealed that H460 cells exhibited strong immunofluorescent signals from cytokeratin-19 fragments (CYFRA 21-1) and squamous cell carcinoma antigen (SCCA) while P1 presented only CYFRA 21-1 signals. We also found evidence of relative cisplatin resistance in P1 compared to the susceptibility level of established lung cancer cells. Thus, the results and methodology described in this study may aid the development of lung cancer diagnostic and therapeutic approaches and, in particular, advance understanding of ethnic differences.

Original languageEnglish (US)
Pages (from-to)252-256
Number of pages5
JournalOncology Letters
Volume4
Issue number2
DOIs
StatePublished - Aug 2012
Externally publishedYes

Fingerprint

Pleural Effusion
Cisplatin
Lung Neoplasms
Tumor Biomarkers
Keratin-19
Neoplasms
Fluorescent Antibody Technique
Cell Biology
Antigens
Drug Therapy
Therapeutics
Growth
Research

Keywords

  • Cancer-associated antigen 125
  • Cisplatin
  • Lung cancer
  • Thai

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Expression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient. / Chanvorachote, Pithi; Luanpitpong, Sudjit; Chunhacha, Preedakorn; Promden, Worrawat; Sriuranpong, Virote.

In: Oncology Letters, Vol. 4, No. 2, 08.2012, p. 252-256.

Research output: Contribution to journalArticle

Chanvorachote, P, Luanpitpong, S, Chunhacha, P, Promden, W & Sriuranpong, V 2012, 'Expression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient', Oncology Letters, vol. 4, no. 2, pp. 252-256. https://doi.org/10.3892/ol.2012.711
Chanvorachote, Pithi ; Luanpitpong, Sudjit ; Chunhacha, Preedakorn ; Promden, Worrawat ; Sriuranpong, Virote. / Expression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient. In: Oncology Letters. 2012 ; Vol. 4, No. 2. pp. 252-256.
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