Expression of cholecystokinin forms by medullary thyroid cancer

G. J. Poston, E. J. Draviam, P. K. Seitz, S. Rajaraman, R. W. Alexander, C. W. Cooper, Courtney Townsend, J. C. Thompson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have studied the production and release of cholecystokinin (CCK) forms by rat medullary thyroid cancer (MTC) in vivo. MTC cells were inoculated s.c. into 8 Wag-Rij rats. One month later, after i.v. injection of calcium plasma levels of CCK, calcitonin and tissue contents of gastrin and calcitonin were determined by radioimmunoassay (RIA), immunocytochemistry, and high-pressure liquid chromatography (HPLC). The tumors were passed 4 times to 8 rats in 1-month intervals fasting levels of CCK in control rats were unaffected by calcium stimulation, and in tumor-bearing rats, plasma CCK was elevated in 3 out of 4 passages, falling to normal levels at the end of passage 4. Hypercalcemia had no effect on plasma levels of CCK in tumor-bearing rats, but did stimulate the release of calcitonin in both control and some tumor-bearing rats in later passages. CCK-8 sulfate was found in all 4 tumor passages but not CCK-33/39. We conclude that rat MTC synthesizes and releases CCK-8, but unlike calcitonin, release of CCK appears unresponsive to calcium stimulation.

Original languageEnglish (US)
Pages (from-to)689-692
Number of pages4
JournalInternational Journal of Cancer
Volume48
Issue number5
StatePublished - 1991

Fingerprint

Cholecystokinin
Calcitonin
Neoplasms
Calcium
Medullary Thyroid cancer
Gastrins
Hypercalcemia
Sulfates
Radioimmunoassay
Fasting
Immunohistochemistry
High Pressure Liquid Chromatography
Injections

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Poston, G. J., Draviam, E. J., Seitz, P. K., Rajaraman, S., Alexander, R. W., Cooper, C. W., ... Thompson, J. C. (1991). Expression of cholecystokinin forms by medullary thyroid cancer. International Journal of Cancer, 48(5), 689-692.

Expression of cholecystokinin forms by medullary thyroid cancer. / Poston, G. J.; Draviam, E. J.; Seitz, P. K.; Rajaraman, S.; Alexander, R. W.; Cooper, C. W.; Townsend, Courtney; Thompson, J. C.

In: International Journal of Cancer, Vol. 48, No. 5, 1991, p. 689-692.

Research output: Contribution to journalArticle

Poston, GJ, Draviam, EJ, Seitz, PK, Rajaraman, S, Alexander, RW, Cooper, CW, Townsend, C & Thompson, JC 1991, 'Expression of cholecystokinin forms by medullary thyroid cancer', International Journal of Cancer, vol. 48, no. 5, pp. 689-692.
Poston GJ, Draviam EJ, Seitz PK, Rajaraman S, Alexander RW, Cooper CW et al. Expression of cholecystokinin forms by medullary thyroid cancer. International Journal of Cancer. 1991;48(5):689-692.
Poston, G. J. ; Draviam, E. J. ; Seitz, P. K. ; Rajaraman, S. ; Alexander, R. W. ; Cooper, C. W. ; Townsend, Courtney ; Thompson, J. C. / Expression of cholecystokinin forms by medullary thyroid cancer. In: International Journal of Cancer. 1991 ; Vol. 48, No. 5. pp. 689-692.
@article{57ae457f14db4a80b69fecda71212c7b,
title = "Expression of cholecystokinin forms by medullary thyroid cancer",
abstract = "We have studied the production and release of cholecystokinin (CCK) forms by rat medullary thyroid cancer (MTC) in vivo. MTC cells were inoculated s.c. into 8 Wag-Rij rats. One month later, after i.v. injection of calcium plasma levels of CCK, calcitonin and tissue contents of gastrin and calcitonin were determined by radioimmunoassay (RIA), immunocytochemistry, and high-pressure liquid chromatography (HPLC). The tumors were passed 4 times to 8 rats in 1-month intervals fasting levels of CCK in control rats were unaffected by calcium stimulation, and in tumor-bearing rats, plasma CCK was elevated in 3 out of 4 passages, falling to normal levels at the end of passage 4. Hypercalcemia had no effect on plasma levels of CCK in tumor-bearing rats, but did stimulate the release of calcitonin in both control and some tumor-bearing rats in later passages. CCK-8 sulfate was found in all 4 tumor passages but not CCK-33/39. We conclude that rat MTC synthesizes and releases CCK-8, but unlike calcitonin, release of CCK appears unresponsive to calcium stimulation.",
author = "Poston, {G. J.} and Draviam, {E. J.} and Seitz, {P. K.} and S. Rajaraman and Alexander, {R. W.} and Cooper, {C. W.} and Courtney Townsend and Thompson, {J. C.}",
year = "1991",
language = "English (US)",
volume = "48",
pages = "689--692",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Expression of cholecystokinin forms by medullary thyroid cancer

AU - Poston, G. J.

AU - Draviam, E. J.

AU - Seitz, P. K.

AU - Rajaraman, S.

AU - Alexander, R. W.

AU - Cooper, C. W.

AU - Townsend, Courtney

AU - Thompson, J. C.

PY - 1991

Y1 - 1991

N2 - We have studied the production and release of cholecystokinin (CCK) forms by rat medullary thyroid cancer (MTC) in vivo. MTC cells were inoculated s.c. into 8 Wag-Rij rats. One month later, after i.v. injection of calcium plasma levels of CCK, calcitonin and tissue contents of gastrin and calcitonin were determined by radioimmunoassay (RIA), immunocytochemistry, and high-pressure liquid chromatography (HPLC). The tumors were passed 4 times to 8 rats in 1-month intervals fasting levels of CCK in control rats were unaffected by calcium stimulation, and in tumor-bearing rats, plasma CCK was elevated in 3 out of 4 passages, falling to normal levels at the end of passage 4. Hypercalcemia had no effect on plasma levels of CCK in tumor-bearing rats, but did stimulate the release of calcitonin in both control and some tumor-bearing rats in later passages. CCK-8 sulfate was found in all 4 tumor passages but not CCK-33/39. We conclude that rat MTC synthesizes and releases CCK-8, but unlike calcitonin, release of CCK appears unresponsive to calcium stimulation.

AB - We have studied the production and release of cholecystokinin (CCK) forms by rat medullary thyroid cancer (MTC) in vivo. MTC cells were inoculated s.c. into 8 Wag-Rij rats. One month later, after i.v. injection of calcium plasma levels of CCK, calcitonin and tissue contents of gastrin and calcitonin were determined by radioimmunoassay (RIA), immunocytochemistry, and high-pressure liquid chromatography (HPLC). The tumors were passed 4 times to 8 rats in 1-month intervals fasting levels of CCK in control rats were unaffected by calcium stimulation, and in tumor-bearing rats, plasma CCK was elevated in 3 out of 4 passages, falling to normal levels at the end of passage 4. Hypercalcemia had no effect on plasma levels of CCK in tumor-bearing rats, but did stimulate the release of calcitonin in both control and some tumor-bearing rats in later passages. CCK-8 sulfate was found in all 4 tumor passages but not CCK-33/39. We conclude that rat MTC synthesizes and releases CCK-8, but unlike calcitonin, release of CCK appears unresponsive to calcium stimulation.

UR - http://www.scopus.com/inward/record.url?scp=0025775721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025775721&partnerID=8YFLogxK

M3 - Article

C2 - 2071230

AN - SCOPUS:0025775721

VL - 48

SP - 689

EP - 692

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 5

ER -