Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages

Li Li, Haimin Feng, Qin Da, Honglin Jiang, Lang Chen, Linlin Xie, Qiuling Huang, Hairong Xiong, Fan Luo, Lei Kang, Yan Zeng, Haitao Hu, Wei Hou, Yong Feng

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.

Original languageEnglish (US)
Pages (from-to)1115-1123
Number of pages9
JournalArchives of virology
Volume161
Issue number5
DOIs
StatePublished - May 1 2016

ASJC Scopus subject areas

  • Virology

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    Li, L., Feng, H., Da, Q., Jiang, H., Chen, L., Xie, L., Huang, Q., Xiong, H., Luo, F., Kang, L., Zeng, Y., Hu, H., Hou, W., & Feng, Y. (2016). Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages. Archives of virology, 161(5), 1115-1123. https://doi.org/10.1007/s00705-016-2755-5