Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages

Li Li, Haimin Feng, Qin Da, Honglin Jiang, Lang Chen, Linlin Xie, Qiuling Huang, Hairong Xiong, Fan Luo, Lei Kang, Yan Zeng, Haitao Hu, Wei Hou, Yong Feng

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.

Original languageEnglish (US)
Pages (from-to)1115-1123
Number of pages9
JournalArchives of Virology
Volume161
Issue number5
DOIs
StatePublished - May 1 2016

Fingerprint

MicroRNAs
HIV-1
Macrophages
HIV
Virus Replication
Viruses

ASJC Scopus subject areas

  • Virology

Cite this

Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages. / Li, Li; Feng, Haimin; Da, Qin; Jiang, Honglin; Chen, Lang; Xie, Linlin; Huang, Qiuling; Xiong, Hairong; Luo, Fan; Kang, Lei; Zeng, Yan; Hu, Haitao; Hou, Wei; Feng, Yong.

In: Archives of Virology, Vol. 161, No. 5, 01.05.2016, p. 1115-1123.

Research output: Contribution to journalArticle

Li, L, Feng, H, Da, Q, Jiang, H, Chen, L, Xie, L, Huang, Q, Xiong, H, Luo, F, Kang, L, Zeng, Y, Hu, H, Hou, W & Feng, Y 2016, 'Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages', Archives of Virology, vol. 161, no. 5, pp. 1115-1123. https://doi.org/10.1007/s00705-016-2755-5
Li, Li ; Feng, Haimin ; Da, Qin ; Jiang, Honglin ; Chen, Lang ; Xie, Linlin ; Huang, Qiuling ; Xiong, Hairong ; Luo, Fan ; Kang, Lei ; Zeng, Yan ; Hu, Haitao ; Hou, Wei ; Feng, Yong. / Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages. In: Archives of Virology. 2016 ; Vol. 161, No. 5. pp. 1115-1123.
@article{ed2a8951f4fb4972bffc3fb13cc8c96e,
title = "Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages",
abstract = "A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.",
author = "Li Li and Haimin Feng and Qin Da and Honglin Jiang and Lang Chen and Linlin Xie and Qiuling Huang and Hairong Xiong and Fan Luo and Lei Kang and Yan Zeng and Haitao Hu and Wei Hou and Yong Feng",
year = "2016",
month = "5",
day = "1",
doi = "10.1007/s00705-016-2755-5",
language = "English (US)",
volume = "161",
pages = "1115--1123",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "5",

}

TY - JOUR

T1 - Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages

AU - Li, Li

AU - Feng, Haimin

AU - Da, Qin

AU - Jiang, Honglin

AU - Chen, Lang

AU - Xie, Linlin

AU - Huang, Qiuling

AU - Xiong, Hairong

AU - Luo, Fan

AU - Kang, Lei

AU - Zeng, Yan

AU - Hu, Haitao

AU - Hou, Wei

AU - Feng, Yong

PY - 2016/5/1

Y1 - 2016/5/1

N2 - A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.

AB - A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.

UR - http://www.scopus.com/inward/record.url?scp=84964262358&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964262358&partnerID=8YFLogxK

U2 - 10.1007/s00705-016-2755-5

DO - 10.1007/s00705-016-2755-5

M3 - Article

VL - 161

SP - 1115

EP - 1123

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 5

ER -