Expression of hydroxamate and phenolate siderophores by Shigella flexneri

S. M. Payne, David Niesel, S. S. Peixotto, K. M. Lawlor

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Shigella flexneri strains were assayed for the ability to synthesize and utilize phenolate and hydroxamate siderophores. The hydroxamate aerobactin was synthesized by all isolates tested, whereas phenolates were only rarely produced. Expression of aerobactin was accompanied by production of a single iron-regulated outer membrane protein (M(r) = 74,000). This protein was not produced by a mutant defective in aerobactin utilization and may serve as the aerobactin receptor. Phenolate (enterobactin)-producing strains synthesized three additional outer membrane proteins (M(r) = 74,000, 81,000, and 83,000) in response to iron starvation. These proteins are the same apparent size as those produced by Escherichia coli K-12 strains. Ent sequences are apparently present in strains which do not synthesize this compound. Although normally silent, ent genes can be activated in Ent- strains to produce Ent+ variants. These laboratory variants are phenotypically indistinguishable from clinical Ent+ isolates.

Original languageEnglish (US)
Pages (from-to)949-955
Number of pages7
JournalJournal of Bacteriology
Volume155
Issue number3
StatePublished - 1983
Externally publishedYes

Fingerprint

Shigella flexneri
Siderophores
Membrane Proteins
Enterobactin
Iron
Starvation
Proteins
Escherichia coli
Genes
aerobactin

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Payne, S. M., Niesel, D., Peixotto, S. S., & Lawlor, K. M. (1983). Expression of hydroxamate and phenolate siderophores by Shigella flexneri. Journal of Bacteriology, 155(3), 949-955.

Expression of hydroxamate and phenolate siderophores by Shigella flexneri. / Payne, S. M.; Niesel, David; Peixotto, S. S.; Lawlor, K. M.

In: Journal of Bacteriology, Vol. 155, No. 3, 1983, p. 949-955.

Research output: Contribution to journalArticle

Payne, SM, Niesel, D, Peixotto, SS & Lawlor, KM 1983, 'Expression of hydroxamate and phenolate siderophores by Shigella flexneri', Journal of Bacteriology, vol. 155, no. 3, pp. 949-955.
Payne, S. M. ; Niesel, David ; Peixotto, S. S. ; Lawlor, K. M. / Expression of hydroxamate and phenolate siderophores by Shigella flexneri. In: Journal of Bacteriology. 1983 ; Vol. 155, No. 3. pp. 949-955.
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