Expression of IL-15 and IL-4 in IFN-γ-independent control of experimental human Cryptosporidium parvum infection

Prema Robinson, Pablo C. Okhuysen, Cynthia L. Chappell, Dorothy E. Lewis, Imran Shahab, Sandeep Lahoti, A. Clinton White

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have previously demonstrated interferon gamma (IFN-γ) in intestinal mucosa after experimental human Cryptosporidium parvum infection, but expression was limited to sensitized volunteers. To characterize IFN-γ-independent mechanisms in control of infection, jejunal biopsies from immunocompetent volunteers experimentally challenged with C. parvum were examined by in situ hybridization for interleukin (IL-)15 and IL-4 mRNA with confirmation by immunohistochemistry. Cytokine expression was correlated with prechallenge anti-C, parvum IgG, symptoms, oocyst shedding, and prior IFN-γ expression data. IL-15 expression was noted only in those without prior sensitization, who did not express IFN-γ. By contrast, expression of IL-4 was associated with prior sensitization. IL-15 was only detected in those with symptoms (6/14 symptomatic vs 0/3 asymptomatic, P>0.05). Among 14 volunteers who did not express IFN-γ, oocyst shedding was lower in those expressing IL-15. Overall, 14/15 volunteers who did not shed oocysts expressed either IFN-γ or IL-15. There was no correlation between expression of IL-4 and symptoms or oocyst shedding. In conclusion, IL-15 expression was associated with control of oocyst shedding in those not expressing IFN-γ. These data suggest that IL-15 is involved in IFN-γ independent mechanisms of control of human cryptosporidiosis, perhaps via activation of the innate immune response.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalCytokine
Volume15
Issue number1
DOIs
StatePublished - Jul 7 2001
Externally publishedYes

Fingerprint

Cryptosporidium parvum
Interleukin-15
Interleukin-4
Interferon-gamma
Oocysts
Infection
Volunteers
Cryptosporidiosis
Biopsy
Intestinal Mucosa
Infection Control
Innate Immunity
In Situ Hybridization
Immunoglobulin G
Chemical activation
Immunohistochemistry
Cytokines
Messenger RNA

Keywords

  • Cryptosporidiosis
  • Cryptosporidium
  • IL-15
  • Intestine
  • Mucosal immunity

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Expression of IL-15 and IL-4 in IFN-γ-independent control of experimental human Cryptosporidium parvum infection. / Robinson, Prema; Okhuysen, Pablo C.; Chappell, Cynthia L.; Lewis, Dorothy E.; Shahab, Imran; Lahoti, Sandeep; White, A. Clinton.

In: Cytokine, Vol. 15, No. 1, 07.07.2001, p. 39-46.

Research output: Contribution to journalArticle

Robinson, Prema ; Okhuysen, Pablo C. ; Chappell, Cynthia L. ; Lewis, Dorothy E. ; Shahab, Imran ; Lahoti, Sandeep ; White, A. Clinton. / Expression of IL-15 and IL-4 in IFN-γ-independent control of experimental human Cryptosporidium parvum infection. In: Cytokine. 2001 ; Vol. 15, No. 1. pp. 39-46.
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