Expression of inflammatory cytokines (interleukin-1β and interleukin-6) in amniochorionic membranes

Ramkumar Menon, Kenneth F. Swan, Timothy W. Lyden, Neal S. Rote, Stephen J. Fortunato

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

OBJECTIVE: This study was designed to investigate the expression of inflammatory cytokines (interleukin-1β and interleukin-6) by fetal membranes in response to infection in vivo and to endotoxin in organ culture. Study design: Amniochorionic membranes were collected from infected and uninfected women and analyzed for cytokine messenger ribonucleic acid and protein. Normal membranes were cultured and exposed to endotoxin. Messenger ribonucleic acid expression was analyzed by reverse transcriptase-polymerase chain reaction. Cellular localization of messenger ribonucleic acid and protein was determined by in situ hybridization and immunocytochemical evaluation, respectively. RESULSTS: Messenger ribonucleic acid for interleukin-1β appeared to be increased in infected or endotoxin-stimulated amniochorionic membranes, whereas interleukin-6 messenger ribonucleic acid was only observed in infected membranes or after endotoxin stimulation. Interleukin-1β messenger ribonucleic acid was localized exclusively to chorionic cells, whereas protein was obsreved in both chorion and amnion. Interleukin-6 messenger ribonucleic acid and protein were produced in both amniotic and chorionic cells. CONCLUSION: Amniochorionic membranes are a site of inflammatory cytokine production. These findings may have significance in preterm labor or premature rupture of membranes.

Original languageEnglish (US)
Pages (from-to)493-500
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume172
Issue number2 PART 1
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Interleukin-1
Interleukin-6
RNA
Cytokines
Membranes
Endotoxins
Proteins
Chorion
Extraembryonic Membranes
Amnion
Premature Obstetric Labor
Organ Culture Techniques
Reverse Transcriptase Polymerase Chain Reaction
In Situ Hybridization
Rupture
Infection

Keywords

  • amniochorionic membranes
  • cytokine
  • Interleukin-1β
  • interleukin-6
  • preterm labor

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Medicine(all)

Cite this

Expression of inflammatory cytokines (interleukin-1β and interleukin-6) in amniochorionic membranes. / Menon, Ramkumar; Swan, Kenneth F.; Lyden, Timothy W.; Rote, Neal S.; Fortunato, Stephen J.

In: American Journal of Obstetrics and Gynecology, Vol. 172, No. 2 PART 1, 1995, p. 493-500.

Research output: Contribution to journalArticle

Menon, Ramkumar ; Swan, Kenneth F. ; Lyden, Timothy W. ; Rote, Neal S. ; Fortunato, Stephen J. / Expression of inflammatory cytokines (interleukin-1β and interleukin-6) in amniochorionic membranes. In: American Journal of Obstetrics and Gynecology. 1995 ; Vol. 172, No. 2 PART 1. pp. 493-500.
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