Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro

Sheila E. Crowe, Luis Alvarez, Marlene Dytoc, Richard H. Hunt, Milan Muller, Philip Sherman, Janak Patel, Yide Jin, Peter B. Ernst

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Background/Aims: Helicobacter pylori is associated with neutrophil infiltrates, although the mechanism of their recruitment is only partially defined. The aim of the study was to determine if Kato III, a human gastric epithelial cell line, expressed cytokines and the intercellular adhesion molecule 1 (ICAM-1), which could contribute to the initiation of inflammation during infection with H. pylori. Methods: Kato III cells were stimulated with H. pylori and were examined for evidence of infection, cytokine production, and the expression of ICAM-1. Results: The expression of interleukin 8 messenger RNA and immunoreactive protein by Kato III cells was significantly increased over constitutive levels within 3 hours of infection with H. pylori. Infected Kato III supernatants activated neutrophils as evidenced by increased CD11b/CD18 and decreased l-selectin that could be blocked by anti-interleukin 8. In contrast, Campylobacter jejuni, lipopolysaccharide, killed H. pylori, and supernatants from cultures of H. pylori did not increase interleukin 8. Interleukins 2 and 6; interferons alfa, beta, and gamma; and tumor necrosis factor were not produced by resting or H. pylori-stimulated Kato III cells. In addition to producing interleukin 8, Kato III constitutively expressed surface ICAM-1, which acts as an intercellular adhesion molecule for neutrophils. Conclusions: Our results indicate that H. pylori stimulates the gastric epithelium to initiate inflammation and neutrophil recruitment and activation.

Original languageEnglish (US)
Pages (from-to)65-74
Number of pages10
JournalGastroenterology
Volume108
Issue number1
DOIs
StatePublished - 1995
Externally publishedYes

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Helicobacter Infections
Interleukin-8
Helicobacter pylori
Stomach
Epithelium
Intercellular Adhesion Molecule-1
Neutrophils
Infection
Cytokines
Inflammation
Selectins
Neutrophil Activation
Campylobacter jejuni
Neutrophil Infiltration
Interferon-beta
Cell Adhesion Molecules
In Vitro Techniques
Interferon-alpha
Interferon-gamma
Interleukin-2

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Crowe, S. E., Alvarez, L., Dytoc, M., Hunt, R. H., Muller, M., Sherman, P., ... Ernst, P. B. (1995). Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro. Gastroenterology, 108(1), 65-74. https://doi.org/10.1016/0016-5085(95)90009-8

Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro. / Crowe, Sheila E.; Alvarez, Luis; Dytoc, Marlene; Hunt, Richard H.; Muller, Milan; Sherman, Philip; Patel, Janak; Jin, Yide; Ernst, Peter B.

In: Gastroenterology, Vol. 108, No. 1, 1995, p. 65-74.

Research output: Contribution to journalArticle

Crowe, SE, Alvarez, L, Dytoc, M, Hunt, RH, Muller, M, Sherman, P, Patel, J, Jin, Y & Ernst, PB 1995, 'Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro', Gastroenterology, vol. 108, no. 1, pp. 65-74. https://doi.org/10.1016/0016-5085(95)90009-8
Crowe, Sheila E. ; Alvarez, Luis ; Dytoc, Marlene ; Hunt, Richard H. ; Muller, Milan ; Sherman, Philip ; Patel, Janak ; Jin, Yide ; Ernst, Peter B. / Expression of interleukin 8 and CD54 by human gastric epithelium after Helicobacter pylori infection in vitro. In: Gastroenterology. 1995 ; Vol. 108, No. 1. pp. 65-74.
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AU - Sherman, Philip

AU - Patel, Janak

AU - Jin, Yide

AU - Ernst, Peter B.

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