Expression of members of the 28-kilodalton major outer membrane protein family of Ehrlichia chaffeensis during persistent infection

Jianzhi Zhang, Hong Guo, Gary M. Winslow, Xue Jie Yu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The 28-kDa immunodominant outer membrane proteins (P28 OMPs) of Ehrlichia chaffeensis are encoded by a multigene family. As an indirect measure of the in vivo expression of the members of thep28 multigene family of E. chaffeensis, sera from two beagle dogs experimentally infected with E. chaffeensis were evaluated for the presence of specific antibodies to P28 OMPs by enzyme-linked immunosorbent assay. Antigenic peptides unique to each of the P28s were identified within the first hypervariable region of each P28 OMP. Serological responses to peptides derived from all P28 OMPs were detected from day 30 postinoculation to day 468 and from day 46 until day 159 in the two beagles. Although antibody titers to the peptides fluctuated, the peak response to all of the peptides appeared simultaneously in each dog. The antibody responses to another outer membrane protein of E. chaffeensis (GP120) showed similar temporal and quantitative changes. These data suggest that the P28 OMPs are expressed concurrently during persistent Ehrlichia infection.

Original languageEnglish (US)
Pages (from-to)4336-4343
Number of pages8
JournalInfection and Immunity
Volume72
Issue number8
DOIs
StatePublished - Aug 2004

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Ehrlichia chaffeensis
Membrane Proteins
Peptides
Multigene Family
Infection
Ehrlichia
Dogs
Antibodies
Antibody Formation
Enzyme-Linked Immunosorbent Assay
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Expression of members of the 28-kilodalton major outer membrane protein family of Ehrlichia chaffeensis during persistent infection. / Zhang, Jianzhi; Guo, Hong; Winslow, Gary M.; Yu, Xue Jie.

In: Infection and Immunity, Vol. 72, No. 8, 08.2004, p. 4336-4343.

Research output: Contribution to journalArticle

Zhang, Jianzhi ; Guo, Hong ; Winslow, Gary M. ; Yu, Xue Jie. / Expression of members of the 28-kilodalton major outer membrane protein family of Ehrlichia chaffeensis during persistent infection. In: Infection and Immunity. 2004 ; Vol. 72, No. 8. pp. 4336-4343.
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