Expression of messenger ribonucleic acid encoding steroidogenic enzymes in postmenopausal ovaries

Manubai Nagamani; Randall Urban

doi:10.1016/S1071-5576(02)00190-9

Expression of messenger ribonucleic acid encoding steroidogenic enzymes in postmenopausal ovaries

Manubai Nagamani, Randall Urban

Internal Medicine

Research output: Contribution to journal › Article

14 Scopus citations

Abstract

Objective: To investigate expression of steroidogenic enzymes involved in androgen and estrogen synthesis in the stroma of postmenopausal ovaries. Methods: Ovarian stromal tissue samples were obtained at hysterectomy from postmenopausal women who had hysterectomy and oophorectomy. Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using ³²P-labeled cDNA probes encoding human P450 side chain cleavage (P450_SSC), P450_17α, and cytochrome P450 aromatase (P450_arom). Concentrations of mRNA encoding cytochrome P450_SSC, cytochrome P450 17α-hydroxylase (CYP17), and P450_arom were determined in the ovarian stroma. Results: We detected P450_SSC mRNA in all postmenopausal ovaries studied. P450_SSC mRNA was increased threefold in the ovarian stroma of postmenopausal women with endometrial cancer and endometrial hyperplasia. CYP17 mRNA was detected in the ovarian stroma of all women with endometrial cancer. P450_arom mRNA was not detected in the ovaries studied. Conclusion: Postmenopausal ovaries possess enzymes for initiation of steroidogenesis. Enzymes essential for androgen synthesis were expressed in the ovarian stroma of postmenopausal women with endometrial cancer or hyperplasia.

Original language	English (US)
Pages (from-to)	37-40
Number of pages	4
Journal	Journal of the Society for Gynecologic Investigation
Volume	10
Issue number	1
DOIs	https://doi.org/10.1016/S1071-5576(02)00190-9
State	Published - Jan 2003

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Keywords

CYP17
Endometrial cancer
Menopausal ovary
P450 side chain cleavage
P450 hydroxylase

ASJC Scopus subject areas

Obstetrics and Gynecology

Cite this

Nagamani, M., & Urban, R. (2003). Expression of messenger ribonucleic acid encoding steroidogenic enzymes in postmenopausal ovaries. Journal of the Society for Gynecologic Investigation, 10(1), 37-40. https://doi.org/10.1016/S1071-5576(02)00190-9

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abstract = "Objective: To investigate expression of steroidogenic enzymes involved in androgen and estrogen synthesis in the stroma of postmenopausal ovaries. Methods: Ovarian stromal tissue samples were obtained at hysterectomy from postmenopausal women who had hysterectomy and oophorectomy. Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled cDNA probes encoding human P450 side chain cleavage (P450SSC), P45017α, and cytochrome P450 aromatase (P450arom). Concentrations of mRNA encoding cytochrome P450SSC, cytochrome P450 17α-hydroxylase (CYP17), and P450arom were determined in the ovarian stroma. Results: We detected P450SSC mRNA in all postmenopausal ovaries studied. P450SSC mRNA was increased threefold in the ovarian stroma of postmenopausal women with endometrial cancer and endometrial hyperplasia. CYP17 mRNA was detected in the ovarian stroma of all women with endometrial cancer. P450arom mRNA was not detected in the ovaries studied. Conclusion: Postmenopausal ovaries possess enzymes for initiation of steroidogenesis. Enzymes essential for androgen synthesis were expressed in the ovarian stroma of postmenopausal women with endometrial cancer or hyperplasia.",

keywords = "CYP17, Endometrial cancer, Menopausal ovary, P450 side chain cleavage, P450 hydroxylase",

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AB - Objective: To investigate expression of steroidogenic enzymes involved in androgen and estrogen synthesis in the stroma of postmenopausal ovaries. Methods: Ovarian stromal tissue samples were obtained at hysterectomy from postmenopausal women who had hysterectomy and oophorectomy. Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled cDNA probes encoding human P450 side chain cleavage (P450SSC), P45017α, and cytochrome P450 aromatase (P450arom). Concentrations of mRNA encoding cytochrome P450SSC, cytochrome P450 17α-hydroxylase (CYP17), and P450arom were determined in the ovarian stroma. Results: We detected P450SSC mRNA in all postmenopausal ovaries studied. P450SSC mRNA was increased threefold in the ovarian stroma of postmenopausal women with endometrial cancer and endometrial hyperplasia. CYP17 mRNA was detected in the ovarian stroma of all women with endometrial cancer. P450arom mRNA was not detected in the ovaries studied. Conclusion: Postmenopausal ovaries possess enzymes for initiation of steroidogenesis. Enzymes essential for androgen synthesis were expressed in the ovarian stroma of postmenopausal women with endometrial cancer or hyperplasia.

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Access to Document

10.1016/S1071-5576(02)00190-9