Expression of messenger ribonucleic acid encoding steroidogenic enzymes in postmenopausal ovaries

Manubai Nagamani; Randall J. Urban

doi:10.1016/S1071-5576(02)00190-9

Expression of messenger ribonucleic acid encoding steroidogenic enzymes in postmenopausal ovaries

Manubai Nagamani, Randall J. Urban

Internal Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Objective: To investigate expression of steroidogenic enzymes involved in androgen and estrogen synthesis in the stroma of postmenopausal ovaries. Methods: Ovarian stromal tissue samples were obtained at hysterectomy from postmenopausal women who had hysterectomy and oophorectomy. Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using ³²P-labeled cDNA probes encoding human P450 side chain cleavage (P450_SSC), P450_17α, and cytochrome P450 aromatase (P450_arom). Concentrations of mRNA encoding cytochrome P450_SSC, cytochrome P450 17α-hydroxylase (CYP17), and P450_arom were determined in the ovarian stroma. Results: We detected P450_SSC mRNA in all postmenopausal ovaries studied. P450_SSC mRNA was increased threefold in the ovarian stroma of postmenopausal women with endometrial cancer and endometrial hyperplasia. CYP17 mRNA was detected in the ovarian stroma of all women with endometrial cancer. P450_arom mRNA was not detected in the ovaries studied. Conclusion: Postmenopausal ovaries possess enzymes for initiation of steroidogenesis. Enzymes essential for androgen synthesis were expressed in the ovarian stroma of postmenopausal women with endometrial cancer or hyperplasia.

Original language	English (US)
Pages (from-to)	37-40
Number of pages	4
Journal	Journal of the Society for Gynecologic Investigation
Volume	10
Issue number	1
DOIs	https://doi.org/10.1016/S1071-5576(02)00190-9
State	Published - Jan 2003

Keywords

CYP17
Endometrial cancer
Menopausal ovary
P450 hydroxylase
P450 side chain cleavage

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/S1071-5576(02)00190-9

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title = "Expression of messenger ribonucleic acid encoding steroidogenic enzymes in postmenopausal ovaries",

abstract = "Objective: To investigate expression of steroidogenic enzymes involved in androgen and estrogen synthesis in the stroma of postmenopausal ovaries. Methods: Ovarian stromal tissue samples were obtained at hysterectomy from postmenopausal women who had hysterectomy and oophorectomy. Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled cDNA probes encoding human P450 side chain cleavage (P450SSC), P45017α, and cytochrome P450 aromatase (P450arom). Concentrations of mRNA encoding cytochrome P450SSC, cytochrome P450 17α-hydroxylase (CYP17), and P450arom were determined in the ovarian stroma. Results: We detected P450SSC mRNA in all postmenopausal ovaries studied. P450SSC mRNA was increased threefold in the ovarian stroma of postmenopausal women with endometrial cancer and endometrial hyperplasia. CYP17 mRNA was detected in the ovarian stroma of all women with endometrial cancer. P450arom mRNA was not detected in the ovaries studied. Conclusion: Postmenopausal ovaries possess enzymes for initiation of steroidogenesis. Enzymes essential for androgen synthesis were expressed in the ovarian stroma of postmenopausal women with endometrial cancer or hyperplasia.",

keywords = "CYP17, Endometrial cancer, Menopausal ovary, P450 hydroxylase, P450 side chain cleavage",

author = "Manubai Nagamani and Urban, {Randall J.}",

year = "2003",

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doi = "10.1016/S1071-5576(02)00190-9",

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AU - Urban, Randall J.

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AB - Objective: To investigate expression of steroidogenic enzymes involved in androgen and estrogen synthesis in the stroma of postmenopausal ovaries. Methods: Ovarian stromal tissue samples were obtained at hysterectomy from postmenopausal women who had hysterectomy and oophorectomy. Tissues were frozen immediately in liquid nitrogen and kept frozen until RNA was extracted. Total RNA was examined by Northern blot analysis using 32P-labeled cDNA probes encoding human P450 side chain cleavage (P450SSC), P45017α, and cytochrome P450 aromatase (P450arom). Concentrations of mRNA encoding cytochrome P450SSC, cytochrome P450 17α-hydroxylase (CYP17), and P450arom were determined in the ovarian stroma. Results: We detected P450SSC mRNA in all postmenopausal ovaries studied. P450SSC mRNA was increased threefold in the ovarian stroma of postmenopausal women with endometrial cancer and endometrial hyperplasia. CYP17 mRNA was detected in the ovarian stroma of all women with endometrial cancer. P450arom mRNA was not detected in the ovaries studied. Conclusion: Postmenopausal ovaries possess enzymes for initiation of steroidogenesis. Enzymes essential for androgen synthesis were expressed in the ovarian stroma of postmenopausal women with endometrial cancer or hyperplasia.

KW - CYP17

KW - Endometrial cancer

KW - Menopausal ovary

KW - P450 hydroxylase

KW - P450 side chain cleavage

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Expression of messenger ribonucleic acid encoding steroidogenic enzymes in postmenopausal ovaries

Abstract

Keywords

ASJC Scopus subject areas

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