Expression of nerve growth factor receptors by human peripheral blood mononuclear cells

B. Morgan, L. W. Thorpe, D. Marchetti, J. R. Perez‐Polo

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    Abstract

    In the rat, nerve growth factor (NGF) has been shown to affect immune reactivity by binding to cell surface receptors on a subpopulation of splenic mononuclear cells. This binding occurs in a specific and saturable fashion to what appear to be low‐affinity (type II) NGF receptors (NGFR). Immunofluorescence studies here showed that NGFR are also present on a proportion of human peripheral blood mononuclear cells (PBMC). Equilibrium binding studies demonstrated that the binding of NGF to its receptors on PBMC occurs with a single equilibrium binding constant (mean) of 2.11 × 10−9 M. The number of receptors per cell was determined to be approximately 6.94 × 103 receptors/cell. These results would suggest a role for NGF in the regulation of immune function in man, as well as in animals.

    Original languageEnglish (US)
    Pages (from-to)41-45
    Number of pages5
    JournalJournal of Neuroscience Research
    Volume23
    Issue number1
    DOIs
    StatePublished - May 1989

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    Keywords

    • cell surface receptors
    • dissociation binding constants
    • immune reactivity
    • lymphocytes

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience

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