Expression of neurotensin messenger RNA in a human carcinoid tumor

B. Mark Evers, Jin Ishizuka, Courtney Townsend, Srinivasan Rajaraman, James C. Thompson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Neurotensin (NT), a distal gut peptide, has important regulatory and trophic effects throughout the gut; however the intracellular mechanisms that regulate the gene expression and release of human NT are not known. The purpose of this endeavor was to study a functioning human pancreatic carcinoid cell line (called BON) in vitro that expresses the NT gene, and to study the effect of the cyclic adenosine monophosphate (cAMP) signal-transduction pathway on the expression and release of human NT. RNA was prepared from BON cell line (which has been established in this laboratory); the RNA was analyzed for NT mRNA expression by Northern hybridization with a complementary DNA probe. RNA blot analysis demonstrated that the NT gene is expressed in BON and is transcribed to two mRNAs of 1.0- and 1.5-kb sizes. In the second part of this study, BON cells were treated with either forskolin (FSK), which increases intracellular levels of cAMP, or with serotonin (S-HT), which reduces cAMP in BON cells. Forskolin produced a dose-dependent increase in NT peptide release and, furthermore, FSK (10-6 mol/L) rapidly increased NT mRNA abundance 1 hour after addition; conversely, 5-HT (10-5 mol/L) decreased NT mRNA at 1 hour. Neurotensin mRNA levels returned to control values by 3 hours after either FSK or 5-HT, which suggests that the transcript half-life for NT is relatively short. These findings show that the expression and peptide release of human NT is mediated, in part, by the cAMP signal-transduction pathway. Our human carcinoid cell line will provide a useful model to study the in vitro regulation of NT gene expression and peptide release.

Original languageEnglish (US)
Pages (from-to)448-454
Number of pages7
JournalAnnals of Surgery
Volume214
Issue number4
StatePublished - Oct 1991

Fingerprint

Neurotensin
Carcinoid Tumor
Messenger RNA
Colforsin
Cyclic AMP
Serotonin
Peptides
RNA
Cell Line
Signal Transduction
DNA Probes
Gene Expression Regulation
Genes
Half-Life

ASJC Scopus subject areas

  • Surgery

Cite this

Evers, B. M., Ishizuka, J., Townsend, C., Rajaraman, S., & Thompson, J. C. (1991). Expression of neurotensin messenger RNA in a human carcinoid tumor. Annals of Surgery, 214(4), 448-454.

Expression of neurotensin messenger RNA in a human carcinoid tumor. / Evers, B. Mark; Ishizuka, Jin; Townsend, Courtney; Rajaraman, Srinivasan; Thompson, James C.

In: Annals of Surgery, Vol. 214, No. 4, 10.1991, p. 448-454.

Research output: Contribution to journalArticle

Evers, BM, Ishizuka, J, Townsend, C, Rajaraman, S & Thompson, JC 1991, 'Expression of neurotensin messenger RNA in a human carcinoid tumor', Annals of Surgery, vol. 214, no. 4, pp. 448-454.
Evers BM, Ishizuka J, Townsend C, Rajaraman S, Thompson JC. Expression of neurotensin messenger RNA in a human carcinoid tumor. Annals of Surgery. 1991 Oct;214(4):448-454.
Evers, B. Mark ; Ishizuka, Jin ; Townsend, Courtney ; Rajaraman, Srinivasan ; Thompson, James C. / Expression of neurotensin messenger RNA in a human carcinoid tumor. In: Annals of Surgery. 1991 ; Vol. 214, No. 4. pp. 448-454.
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